Primer set, application, product and method for detecting SNP sites related to nutrient metabolism ability

Abstract

The invention relates to the technical field of biology and provides a nutrition metabolic capacity related SNP site detection primer group, application, product and method. The nutrition metabolic capacity related SNP site detection primer group can be used for detecting vitamin A metabolic capacity, vitamin B6 metabolic capacity, vitamin B12 metabolic capacity, vitamin C metabolic capacity, vitamin D metabolic capacity, vitamin E metabolic capacity, elemental calcium metabolic capacity, elemental iron metabolic capacity, elemental zinc metabolic capacity, elemental selenium metabolic capacity, elemental iodine metabolic capacity, elemental sodium metabolic capacity, alcohol metabolic capacity, folic acid metabolic capacity, lactose metabolic capacity, unsaturated fatty acid metabolic capacity, saturated fatty acid metabolic capacity, cholesterol metabolic capacity, carbohydrate metabolic capacity and caffeine metabolic capacity. By the primer group, specific detection can be realized, high accuracy is achieved, a detection period can be shortened sharply, and detection cost is reduced.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a primer set, application, product and method for detecting SNP sites related to nutrient metabolism. Background technique [0002] Nutrient elements play an irreplaceable role in the human body and are necessary to maintain good health. They mainly include vitamins, trace mineral elements and other nutrients such as lactose, cholesterol, and carbohydrates. Lack of nutrients and excessive supplementation can cause physiological disorders or cause other diseases. A large number of studies have shown that the absorption and metabolism of a variety of nutrient elements are related to gene polymorphism, and gene mutations will cause differences in the activity of synthetic metabolic enzymes, which will cause differences in the body's metabolism and absorption of nutrients. Therefore, genetic testing can assess the individual's ability to absorb and metabolize different nutrients, and...

AI Technical Summary

Problems solved by technology

However, the above methods all have some shortcomings or deficiencies. For example, although Sanger sequencing is the gold standard for sequencing and can detect unknown mutations, this method has disadvantages such as low throughput, cumbersome operation, and high sample loading; Taqman method can only detect Known mutations, and the price of probe synthesis is high; ARMS method has high detection sensitivity, but the design of primers is difficult, and the requirements for technicians are high, etc.

[0004]In summary, it is found that the tests related to nutrient metabolism are all based on a certain type or a certain nutrient element, and it is impossible to comprehensively evaluate the individual's absorption of multiple nutrient elements and metabolic capacity

Images