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Multi-spectral stem cell activity detection and analysis system based on hollow-core optical fiber

A hollow-core optical fiber and activity detection technology, applied in the field of stem cell activity detection and analysis system, can solve the problems of low efficiency, high cost, cumbersome and complicated operation, etc., and achieve the effect of low cost, simple structure and improved detection efficiency

Pending Publication Date: 2021-10-22
安徽中实华粹医疗器械有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these biochemical methods use consumable reagents, which are not only costly, but also complicated and inefficient to operate.

Method used

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  • Multi-spectral stem cell activity detection and analysis system based on hollow-core optical fiber
  • Multi-spectral stem cell activity detection and analysis system based on hollow-core optical fiber

Examples

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Comparison scheme
Effect test

Embodiment 1

[0019] In this example, two groups (group A and group B) of different active stem cell suspensions were used, and before the surface enhancement test was performed, a part of the cells were taken out for MTT test to determine their relative activity. The absorbances of group A and group B were A=0.82 and AB=0.51 respectively by MTT test, showing that the cell activity of group A is relatively high. Aspirate the cell suspensions of Group A and Group B into the hollow-core optical fiber. A 633nm laser was used to output excitation light with a power of 1mW, and its spectrum was measured by Raman scattering / infrared / visible light / terahertz multispectral. Acquired spectra were baseline corrected. Multi-spectral analysis showed that group A with high activity had stronger multi-spectral signal and more obvious peak position, while group B with low activity had weaker spectral signal and less obvious peak position than group A. Therefore, the activity of stem cells can be determin...

Embodiment 2

[0022] In this example, two groups (group A and group B) of different active stem cell suspensions were used, and before the surface enhancement test was performed, a part of the cells were taken out for MTT test to determine their relative activity. The absorbances of group A and group B were A=0.82 and AB=0.51 respectively by MTT test, showing that the cell activity of group A is relatively high. Aspirate the cell suspensions of Group A and Group B into the hollow-core optical fiber. Use a 633nm laser to output 1mW of excitation light, and use a hollow-core fiber modified with gold nanoparticles, such as figure 2 , and its Raman spectrum was measured with a Raman spectrometer. The acquired Raman spectra were averaged after baseline correction to obtain the average Raman spectrum. The signal intensity of Raman spectrum is significantly higher than that of the hollow core fiber without modified nanoparticles. And after a year-on-year comparison, the Raman spectrum of Group...

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Abstract

The invention discloses a multispectral stem cell activity detection and analysis system based on a hollow-core optical fiber. The system comprises a cell container used for placing stem cells to be detected. a hollow-core optical fiber stretches into the cell container, and an optical fiber connected with an objective lens of a Raman spectrum device is connected with the hollow-core optical fiber through an optical fiber coupling part; the Raman spectrum device is connected with a computer for analyzing the activity of the stem cells; a light irradiation device for irradiating the stem cells to be detected in the hollow-core optical fiber and exciting Raman scattering is additionally arranged; and the inner surface of the hollow-core optical fiber adsorbs enhanced substrate nanoparticles for enhancing a Raman detection signal. The system can greatly improve the detection efficiency of stem cell activity, does not need fluorescent staining, is non-toxic to cells, has the advantages of low cost, rapidness and simplicity, and has good application prospects in the fields of disease treatment, artificial reproduction, organ transplantation and beauty.

Description

technical field [0001] The invention relates to a stem cell activity detection and analysis system applicable in the fields of medical treatment, pharmacy and life science research. Background technique [0002] Stem cells are undifferentiated biological cells found in multicellular organisms that can both differentiate into specialized cells and produce more stem cells through mitosis. In mammals, two types of stem cells exist: embryonic stem cells, which separate from the inner cell mass of blast cells, and adult stem cells, which are found in different tissues. In adults, stem and progenitor cells serve as the body's repair system, replenishing adult tissues. In the developing embryo, stem cells can differentiate into all specialized cells - ectoderm, endoderm and mesoderm, but also maintain the normal metabolism of regenerating organs such as blood, skin or intestinal tissue. The activity of stem cells not only affects their ability to grow and proliferate, but also de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 刘书朋赵宝库田静姜剑国刘新国
Owner 安徽中实华粹医疗器械有限公司
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