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Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor

A technology for fermentation liquid and licorice extract, applied in the field of microorganisms, can solve problems such as rupture of plant cell walls, and achieve the effects of reducing blood lipid concentration, increasing content and increasing abundance

Active Publication Date: 2021-10-26
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing traditional Chinese medicine processing methods often cannot give full play to the advantages of traditional Chinese medicine. The fermentation of traditional Chinese medicine can use microorganisms such as bacteria and fungi to decompose traditional Chinese medicine. The fermentation process will induce the rupture of plant cell walls, resulting in the release or synthesis of various antioxidant compounds. Improve the utilization rate of traditional Chinese medicine and enhance the efficacy

Method used

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  • Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor
  • Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor
  • Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor

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preparation example Construction

[0040] The invention provides a kind of preparation method of licorice fermented liquid, comprises the following steps:

[0041] (1) Preparation of licorice extract: licorice and water are mixed and soaked, directly decocted after soaking, filtered to obtain a primary filtrate and a primary filter residue, adding water to the primary filter residue to continue decocting, filtering to obtain a secondary filtrate, and combining the two filtrates, Concentrate, centrifuge, and sterilize to obtain licorice extract;

[0042] (2) Fermentation: the licorice extract in step (1) is mixed with the Lactobacillus plantarum liquid, fermented, and filtered to obtain the licorice fermented liquid.

[0043] In the present invention, the soaking time in step (1) is preferably 8-10 hours, more preferably 8.5-9.5 hours.

[0044] In the present invention, the volume ratio of decocting licorice and water for the first time in step (1) is preferably 1:5-15, more preferably 1:8-12; the decocting tim...

Embodiment 1

[0053] The present embodiment provides a kind of preparation method of licorice fermented liquid, comprises the following steps:

[0054] (1) Preparation of licorice extract: Weigh the licorice sample, add 10 times the volume of purified water to mix, soak for 9 hours, decoct for 2 hours after soaking, and repeat the filtration 3 times to obtain a filtrate and a filter residue, and add 8 times the volume to the filter residue Continue to decoct the volume of purified water for 1 hour, filter to obtain the second filtrate, combine the two filtrates, concentrate in a water bath at 100°C, centrifuge at 4°C and 3000rmp / min for 5 minutes, take the supernatant, and sterilize the supernatant at 121°C for 20 minutes to obtain licorice Extraction solution;

[0055] (2) Strain activation: Prepare MRS liquid medium according to the instructions, sterilize at 121°C for 20 minutes, add 1mL of MRS liquid medium into a sterile centrifuge tube, pick a single colony of Lactobacillus plantarum,...

Embodiment 2

[0058] The present embodiment provides a kind of preparation method of licorice fermented liquid, comprises the following steps:

[0059] (1) Preparation of licorice extract: Weigh the licorice sample, add 5 times the volume of purified water to mix, soak for 10 hours, decoct for 1 hour after soaking, repeat the filtration 3 times to obtain a filtrate and a filter residue, and add 5 times the volume to the filter residue Continue to decoct the volume of purified water for 0.5h, filter to obtain the second filtrate, combine the two filtrates, concentrate in a water bath at 90°C, centrifuge at 0°C and 2000rmp / min for 10min, take the supernatant, and sterilize the supernatant at 121°C for 20min to obtain Licorice extract;

[0060] (2) Strain activation: Prepare MRS liquid medium according to the instructions, sterilize at 121°C for 20 minutes, add 1mL of MRS liquid medium into a sterile centrifuge tube, pick a single colony of Lactobacillus plantarum, mix well, and incubate at 37...

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Abstract

The invention belongs to the technical field of microorganisms, and provides liquorice fermentation liquor with the effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of the liquorice fermentation liquor. The licorice fermentation liquor is obtained by fermenting licorice through lactobacillus plantarum. The lactobacillus plantarum is lactobacillus plantarum LZU-S-ZCJ, the lactobacillus plantarum is preserved in the Guangdong Microbial Culture Collection Center on December 31, 2020, and the preservation number is GDMCCNO: 61402; and the nucleotide sequence of the lactobacillus plantarum is as shown in SEQ: NO.1. The licorice fermentation liquor has good effects of resisting oxidation and relieving acute alcoholic liver injury, can remove DPPH free radicals, hydroxyl free radicals, ABTS free radicals and reduced iron ions, and has a good regulation effect on intestinal flora.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a licorice fermented liquid capable of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and its application. Background technique [0002] Alcohol-induced oxidative stress refers to an increase in the proportion of reactive oxygen species (Reactive Oxygen Species, ROS) in the body, or a decrease in the activity of antioxidants and antioxidant enzymes under acute or chronic alcohol exposure. After excessive alcohol intake, the balance between the original oxidation and reduction system in the body will be broken. When there is not enough antioxidants in the liver to consume the excess ROS, ROS will accumulate in large quantities. Due to the short half-life of ROS, Strong reactivity, it will quickly combine with ethanol or iron atoms to form hydroxyl radicals, ferrous oxide or hydroxyethyl radicals, which will lead to peroxidation of cell...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K36/484A61P39/06A61P1/00A61P1/16C12R1/25
CPCA61K36/484A61P39/06A61P1/00A61P1/16A61K2236/19A61K2236/331A61K2236/39Y02A50/30
Inventor 张春江杨耘林洋
Owner LANZHOU UNIVERSITY
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