Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor

A technology for fermentation liquid and licorice extract, applied in the field of microorganisms, can solve problems such as rupture of plant cell walls, and achieve the effects of reducing blood lipid concentration, increasing content and increasing abundance

Active Publication Date: 2021-10-26
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing traditional Chinese medicine processing methods often cannot give full play to the advantages of traditional Chinese medicine. The fermentation of traditional Chinese medicine can use microorganisms such as bacteria and fungi to decompos

Method used

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  • Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor
  • Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor
  • Liquorice fermentation liquor with effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of liquorice fermentation liquor

Examples

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Example Embodiment

[0040] The present invention provides a method of preparing a licorice fermentation broth, comprising the steps of:

[0041] (1) Preparation of licorice extract: licorice is mixed with water, soak directly, filter, filter, a filtrate and a filter residue, and then add water in a filter residue to continue to boil, filter, obtain secondary filtrate, combined with two filtrates, EtOAc EtOAc (EtOAc)

[0042] (2) Fermentation: The licorice extract of step (1) is mixed with the plant Lactobacillus liquid, fermented, and filtered to give a licorice fermentation liquid.

[0043] In the present invention, the soaking time in step (1) is preferably 8 to 10 h, more preferably 8.5 to 9.5 h.

[0044] In the present invention, the volume ratio of the first decoction of glycycetic and water in step (1) is preferably from 1: 5 to 15, more preferably from 1: 8 to 12; the decoction time is preferably from 0.5 to 2 h, more preferably 1 ~ 1.5h.

[0045] In the present invention, the volume ratio of ...

Example Embodiment

[0052] Example 1

[0053] This embodiment provides a method of preparing a licorice fermentation broth, including the following steps:

[0054] (1) Preparation of the licorice extract: Glycyldicose sample, add 10 times volume purified water mix, soak for 9 h, soak directly decoction 2H, repeat filtration 3 times, to obtain a filtration, 8 times in a filter residue The volume purified water continued to cook for 1 h, filtered, obtained a secondary filtrate, combined with the filtrate, concentrated at 100 ° C, centrifugation at 4 ° C, 3 MIN, was taken into the supernatant, the supernatant 121 ° C was sterilized for 20 min after 20 min Extract;

[0055] (2) Activation of strains: 1 ml of MRS liquid medium is added in accordance with the instructions, 121 ° C, sterilized for 20 min, sterile centrifugal tube, and the plant lactobacterium single colonies were added, mixed, 37 ° C culture 20h To obtain a generation of bacteria, a 100 μL of a generation of bacterial solution was obtained ...

Example Embodiment

[0057] Example 2

[0058] This embodiment provides a method of preparing a licorice fermentation broth, including the following steps:

[0059] (1) Preparation of licorice extract: Glycyldose sample, add 5 times volume purified water mix, soak for 10 h, soak directly decoction 1H, repeat filtration 3 times, to obtain a filtration, 5 times in one filter residue The volume purified water continued to cook for 0.5 h, filtered to give a secondary filtrate, combined with the filtrate, concentrated at 90 ° C, centrifuged at 0 ° C, centrifugation for 10 min, was taken up, and the supernatant was sterilized for 20 minutes. Licorice extract;

[0060] (2) Activation of strains: 1 ml of MRS liquid medium is added in accordance with the instructions, 121 ° C, sterilized for 20 min, sterile centrifugal tube, and the plant lactobacterium single colonies were added, mixed, 37 ° C culture 20h To obtain a generation of bacteria, a 100 μL of a generation of bacterial solution was obtained from 4.9 ...

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Abstract

The invention belongs to the technical field of microorganisms, and provides liquorice fermentation liquor with the effects of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and application of the liquorice fermentation liquor. The licorice fermentation liquor is obtained by fermenting licorice through lactobacillus plantarum. The lactobacillus plantarum is lactobacillus plantarum LZU-S-ZCJ, the lactobacillus plantarum is preserved in the Guangdong Microbial Culture Collection Center on December 31, 2020, and the preservation number is GDMCCNO: 61402; and the nucleotide sequence of the lactobacillus plantarum is as shown in SEQ: NO.1. The licorice fermentation liquor has good effects of resisting oxidation and relieving acute alcoholic liver injury, can remove DPPH free radicals, hydroxyl free radicals, ABTS free radicals and reduced iron ions, and has a good regulation effect on intestinal flora.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a licorice fermented liquid capable of resisting oxidation, relieving acute alcoholic liver injury and regulating intestinal flora and its application. Background technique [0002] Alcohol-induced oxidative stress refers to an increase in the proportion of reactive oxygen species (Reactive Oxygen Species, ROS) in the body, or a decrease in the activity of antioxidants and antioxidant enzymes under acute or chronic alcohol exposure. After excessive alcohol intake, the balance between the original oxidation and reduction system in the body will be broken. When there is not enough antioxidants in the liver to consume the excess ROS, ROS will accumulate in large quantities. Due to the short half-life of ROS, Strong reactivity, it will quickly combine with ethanol or iron atoms to form hydroxyl radicals, ferrous oxide or hydroxyethyl radicals, which will lead to peroxidation of cell...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K36/484A61P39/06A61P1/00A61P1/16C12R1/25
CPCA61K36/484A61P39/06A61P1/00A61P1/16A61K2236/19A61K2236/331A61K2236/39Y02A50/30
Inventor 张春江杨耘林洋
Owner LANZHOU UNIVERSITY
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