Nano armored protective single-cell product for treating Parkinson's disease through bacteria and preparation method thereof

An armor protection and single-cell technology, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve problems such as probiotic destruction, bacterial death, and limited GABA production capacity, and achieve Reduce inflammatory damage, acceptable effect

Active Publication Date: 2021-10-29
TANGYI HLDG(SHENZHEN) LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strong acid and active enzymatic environment of gastric juice, probiotics can be severely damaged, resulting in structural damage and loss of biological activity
Therefore, after reaching the intestinal tract, most of the bacteria died or were seriously injured, and their metabolic ability was severely damaged, resulting in limited GABA productio

Method used

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  • Nano armored protective single-cell product for treating Parkinson's disease through bacteria and preparation method thereof
  • Nano armored protective single-cell product for treating Parkinson's disease through bacteria and preparation method thereof
  • Nano armored protective single-cell product for treating Parkinson's disease through bacteria and preparation method thereof

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Embodiment 1

[0033] A nano-armoured protective single-cell product, including liposomes and probiotics encapsulated by liposomes. Liposomes are widely used in drug delivery. The raw materials of liposomes, phospholipids and cholesterol, are stable in the mouth and stomach. After the small intestine, it can only be decomposed under the catalysis of lipase. Liposomes choose soybean lecithin and cholesterol, because they can maintain a stable state in the mouth and stomach, and can be decomposed in the small intestine, exposing the probiotics wrapped in it. It has been verified that liposomes can form a stable lipid film on the surface of BA, which can provide living cells with a strong armor against gastric juice and ensure the activity of cells.

[0034] A method for preparing a nano-armor-protected single-cell product, comprising the following steps:

[0035] S1. Preparation of liposomes: Weigh soybean lecithin, cholesterol and vitamin E in 4:2:1, place in chloroform solvent, stir at room...

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Abstract

The invention discloses a nano armored protective single-cell product, which comprises liposome and probiotics wrapped by the liposome, the probiotics are fermented to generate gamma-aminobutyric acid (GABA), the gamma-aminobutyric acid (GABA) alleviates the activation of a nigra inflammatory reaction caused by lipopolysaccharide (LPS) in a PD model, so that the gamma-aminobutyric acid alleviates the inflammatory damage of nigra dopaminergic neurons, and plays a role in protecting nerves, and the probiotics are wrapped by the lipidosome, so that the probiotics are protected, and the probiotics are prevented from being damaged by strong acid in gastric acid and digestive enzymes. According to the nano armored protective single-cell product for treating Parkinson's disease through bacteria and the preparation method thereof, the advantages of nano armored and bacterial treatment are fully utilized, and a single living cell is decorated by a nano-armored coating, so that the nano armored protective single-cell product can not only prevent toxic and side effects caused by overhigh local GABA concentration, but also can maintain the concentration of the GABA in a stable range in vivo, the half-life period of the GABA is greatly prolonged, and the neurological rehabilitation effect is greatly improved.

Description

technical field [0001] The invention relates to the technical field of nano-armored single-cell protection, in particular to a nano-armored single-cell product for treating Parkinson's disease through bacteria and a preparation method thereof. Background technique [0002] Parkinson's disease, as the second largest neurodegenerative disease in the world, is mainly related to a variety of neuropathological injuries, such as degeneration of dopaminergic neurons, neuroinflammation, and apoptosis. Clinically, the main symptoms of Parkinson's disease are static tremor and slow movement caused by neuronal damage. In fact, gastrointestinal symptoms in patients with Parkinson's disease often precede neurologic symptoms. The researchers found that the richness of gut microbiota in patients with Parkinson's disease was significantly different from that in normal people. An increasing number of studies have shown that the gut microbiota in the gut-brain-microbe axis plays a crucial r...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61K35/745A61K9/127A61K47/24A61K47/28A61K47/22A61P25/16
CPCA61K35/741A61K35/745A61K9/127A61K9/1277A61K47/24A61K47/28A61K47/22A61P25/16Y02A50/30A61K2035/115
Inventor 郑斌曹毓琳孙维郭庆陆程世翔
Owner TANGYI HLDG(SHENZHEN) LTD
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