Application of small molecule compound composition in preparation of medicine for preventing and treating retinal injury diseases

A small molecule compound and retinal damage technology, which can be applied in drug combinations, sensory diseases, cardiovascular system diseases, etc., can solve problems such as poor curative effect, achieve the effect of protecting structural integrity and normal function, and preventing retinal damage

Active Publication Date: 2021-11-02
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For dry AMD, there are currently no effective

Method used

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  • Application of small molecule compound composition in preparation of medicine for preventing and treating retinal injury diseases
  • Application of small molecule compound composition in preparation of medicine for preventing and treating retinal injury diseases
  • Application of small molecule compound composition in preparation of medicine for preventing and treating retinal injury diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, crystal violet staining and taking pictures in bright field

[0029] 1. Experimental materials and procedures

[0030] 1.1 Cell culture medium preparation: DMEM / F12+10% fetal bovine serum+1% P / S double antibody (penicillin+streptomycin).

[0031] 1.2 Combination preparation of small molecule compounds:

[0032] NU7441: Dilute NU7441 powder with ultrapure water, and the final concentration of the mother solution is 3mM.

[0033] Ergothioneine: Dilute the ergothioneine powder with ultrapure water, and the final concentration of the mother liquor is 30mM.

[0034] RepSox: Dilute NU7441 powder with ultrapure water, and the final concentration of the mother solution is 3mM.

[0035] Mix the three small molecule compound mother liquors in equal volumes to form a small molecule compound combination.

[0036] 1.3 Preparation of sodium iodate: Sodium iodate (SI) was directly diluted with culture medium, and the final concentration was 10 μM.

[0037] 1.4 Treat...

Embodiment 2

[0050] Embodiment 2, cell viability experiment (CCK8 staining)

[0051] (1) method

[0052] 1. Experimental materials and procedures

[0053] 1.1 Cell culture medium preparation: DMEM / F12+10% fetal bovine serum+1% P / S double antibody.

[0054] 1.2 Combination preparation of small molecule compounds:

[0055] NU7441: Dilute NU7441 powder with ultrapure water, and the final concentration of the mother solution is 3mM.

[0056] Ergothioneine: Dilute the ergothioneine powder with ultrapure water, and the final concentration of the mother liquor is 30mM.

[0057] RepSox: Dilute NU7441 powder with ultrapure water, and the final concentration of the mother solution is 3mM.

[0058] Mix the three small molecule compound mother liquors in equal volumes to form a small molecule compound combination.

[0059] 1.3 Sodium iodate preparation: Weigh sodium iodate powder, dissolve it in an appropriate amount of culture medium and directly dilute, the final use concentration is 10 μM.

...

Embodiment 3

[0068] Embodiment 3, animal experiment-RPE cell layer spreading

[0069] (1) method

[0070] 1. Animal Injection

[0071] 1.1 The injected animals are male C57 / BL6 mice of 4 to 6 weeks. The experiment is divided into: negative control group CTR (PBS), model control group (sodium iodate), and experimental group is sodium iodate + small molecule compound combination co-treatment group (NU7441 concentration is 8 mg / kg; ergothioneine concentration is 80 mg / kg; RepSox concentration is 8 mg / kg).

[0072] 1.2 Intraperitoneal injection of sodium iodate: Sodium iodate was dissolved in PBS, calculated according to the weight of the animal, and the injection dose for each animal was 25 mg / kg.

[0073] 1.3 Intraperitoneal injection of small molecule compound injection: Dissolve the small molecule in PBS to make mother solution and then dilute with equal volume. Calculated according to the weight of the animal, the final concentration of the injection dose for each animal is NU74418mg / kg...

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Abstract

The invention relates to the technical field of biology, and particularly discloses application of a small molecule compound composition in preparation of a medicine for preventing and treating retinal injury diseases. The small molecule compound composition effectively antagonizes cell death and dysfunction caused by oxidative stress on retinal pigment epithelial cells, and can effectively protect the retinal pigment epithelial cells so as to protect the structural integrity and normal function of the whole retinal layer cells; the compound can be used for preventing and potentially treating retinal injury caused by oxidative stress, and is particularly favorable for preventing and/or treating age-related maculopathy and other retinal injury diseases. Besides, experiments prove that the small molecule compound composition has no obvious pharmacological toxicity and can effectively control occurrence and development of retinal injury diseases, and a new theoretical support is provided for development of unknown biological activity and future clinical treatment effects of the small molecule compound composition.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a combination of small molecular compounds in the preparation of drugs for the prevention and treatment of retinal damage diseases. Background technique [0002] Age-related macular degeneration (AMD), a disease that causes loss of central visual field, abnormal color and low-light vision, and visual degeneration, is the leading cause of blindness in the elderly population. The macula is a special structure with a diameter of about 1.5 mm located in the center of the posterior pole of the retina. It is mainly responsible for fine vision and color vision. As the most sensitive part of vision, once the macula is damaged, it will seriously affect the central vision. Obstacles to daily activities such as face recognition, driving, and reading. AMD is divided into wet AMD and dry AMD. Wet AMD manifests as choroidal neovascularization, detachment of the retinal pigment ...

Claims

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Application Information

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IPC IPC(8): A61K31/5377A61K45/06A61P9/10A61P27/02A61P39/06A61K31/4172A61K31/444
CPCA61K31/4172A61K31/5377A61K31/444A61K45/06A61P27/02A61P9/10A61P39/06A61K2300/00
Inventor 欧阳宏王力
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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