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Fluorescent probe based on polyoxometallate and assembly of polyoxometallate and application of fluorescent probe in spermine detection

A polyoxometalate, fluorescent probe technology, applied in the field of fluorescent probes, achieves the effects of high sensitivity, simple operation, and no need for pretreatment

Active Publication Date: 2021-11-02
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the similar structures and properties of biopolyamines pose great challenges for identification

Method used

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  • Fluorescent probe based on polyoxometallate and assembly of polyoxometallate and application of fluorescent probe in spermine detection
  • Fluorescent probe based on polyoxometallate and assembly of polyoxometallate and application of fluorescent probe in spermine detection
  • Fluorescent probe based on polyoxometallate and assembly of polyoxometallate and application of fluorescent probe in spermine detection

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Experimental program
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Effect test

Embodiment 1

[0029] Polyoxometalates (EuW 10 ) Fluorescence probe detects the method for spermine (Spm) in the solution: the EuW that concentration is 2.0mmol / L 10 Dilute the mother liquor to 40 times the volume with the configured pH=6.5 MES-NaOH buffer solution, and prepare EuW 10 A solution with a concentration of 50.0 μmol / L. Take 1 mL of the fluorescent probe solution, and add different types of polyamine mother liquors to each 1 mL of the fluorescent probe solution, so that the final concentrations are 0-250 μmol / L (0, 0.25, 0.50, 1.00, 1.50, 2.00 , 2.50, 3.00, 3.50, 5.00, 10.0, 15.0, 25.0, 35.0, 50.0, 100, 150, 200, 250 μmol / L), and use a fluorescence spectrometer to record the fluorescence emission spectra of the fluorescent probe solution in response to different concentrations of polyamines (Excitation wavelength is 500nm). Such as figure 1 (Put), figure 2 (Spd) and image 3 As shown in (Spm), with the increase of the concentration of different types of polyamines, their f...

Embodiment 2

[0031] EuW with a concentration of 2.0mmol / L 10 The mother liquor was diluted to 200 times the volume with the prepared pH=6.5 MES-NaOH buffer solution to prepare a solution with a low concentration of 10.0 μmol / L, and 1 mL was used as the fluorescent probe solution. Add spermine (Spm) mother solution with a concentration of 10.0mmol / L to each 1mL fluorescent probe solution, so that the final concentrations are 0.05, 0.10, 0.20, 0.30, 0.40, 0.50, 0.60μmol / L, and use fluorescence Spectrometer records the fluorescence emission spectrum (excitation wavelength is 500nm) of this fluorescent probe solution to the Spm response of different concentrations (as Figure 6 shown). And draw the relational graph of the fluorescence emission intensity (591nm) of this system and Spm concentration, further can obtain this EuW by linear fitting 10 The fluorescence detection linear relationship to Spm (such as Figure 7 Shown), it is found that it has a good linear dependence on the Spm conce...

Embodiment 3

[0033] Build EuW 10 / GL-22 fluorescent probe assembly: Take 1 mL of EuW prepared in Example 1 with a concentration of 50.0 μmol / L 10 Fluorescent probe solution, add different concentrations of GL-22 to each 1mL fluorescent probe solution (solid GL-22 is dissolved in ultrapure water to prepare 2.0mmol / / L stock solution), the final concentration of GL-22 is respectively 0~50.0μmol / L (0, 0.5, 2.5, 5.0, 10.0, 15.0, 20.0, 25.0, 30.0, 35.0, 40.0, 45.0, 50.0μmol / L), and use the fluorescence spectrometer to record the concentration of the fluorescent probe solution The fluorescence emission spectrum of the GL-22 response (excitation wavelength is 500nm). Such as Figure 8 As shown, with the increase of GL-22 concentration, its fluorescence emission peak intensity at 591nm is gradually enhanced. Simultaneously, by drawing the relational curve of this system fluorescence emission intensity (591nm) and GL-22 concentration (such as Figure 9 shown), we found that it has a good linear ...

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Abstract

The invention discloses a fluorescent probe based on polyoxometallate and an assembly thereof and an application of the fluorescent probe in spermine detection, and belongs to the technical field of fluorescent probes. According to the invention, a supramolecular assembly strategy is utilized, environment-sensitive polyoxometallate EuW10 is taken as a fluorophore, a peptide fragment GL-22 of HPV E6 is introduced to construct an assembly, and specific recognition and detection of spermine (Spm) are realized. The EuW10 / GL-22 assembly can be used for effectively distinguishing spermine (Spm) and spermidine (Spd), so that the specific response to the Spm is realized. The linear response range of the EuW10 / GL-22 assembly to spermine is 0.05 to 0.6 [mu] mol / L, and the detection limit is 2.2 nmol / L. The method is quick in response, high in sensitivity and strong in specificity in the aspect of detecting trace spermine biomarkers. Therefore, the detection performance of the probe can be effectively improved through a supramolecular assembly strategy, and high-sensitivity detection of the biogenic polyamine is realized.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, in particular to a fluorescent probe based on polyoxometalate and its assembly and its application in detecting spermine. Background technique [0002] Spermine (Spermine, Spm) is a typical polyamine that exists in eukaryotic cells of all organisms and plays an important role in cell growth, proliferation and even protein synthesis. Increased Spm content in urine is directly related to malignancy, which makes it a marker of tumorigenesis and an important indicator of chemotherapy. In addition, spermine is also a key factor in food toxicity, and it can lead to food deterioration at higher concentrations and cause food poisoning. In view of the fact that the Spm level is an important indicator for judging human health and clinical diagnosis, it is of great significance to develop an efficient detection strategy for spermine. However, the similar structures and properties of biopolyamin...

Claims

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Application Information

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IPC IPC(8): C09K11/78C09K11/02G01N21/64
CPCC09K11/7736C09K11/025G01N21/6428
Inventor 吴玉清张春霞李洪伟
Owner JILIN UNIV
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