Efficient tissue culture breeding method for ficus hirta

Abstract

The invention discloses an efficient tissue culture breeding method for ficus hirta. The efficient tissue culture breeding method comprises the steps of plant pretreatment, explant selection and sterilization, primary induction culture, subculture multiplication culture, rooting culture and acclimatization and transplantation. Specifically, robust, pest-free and excellent-character current-year young stem segments of ficus hirta are selected as an explant material source, an explant is sterilized and pruned, the explant is inoculated into a primary induction culture medium to obtain initial buds, then the initial buds are inoculated into a subculture multiplication culture medium to form clumpy buds, single buds are cut, the single buds are vertically inserted into a rooting culture medium to induce rooting, and finally, acclimatizing and transplanting are carried out to obtain tissue culture survival seedlings of the ficus hirta. The efficient tissue culture breeding method for the ficus hirta is simple in operation process, short in culture period, high in multiplication coefficient and rooting rate and good in seedling quality, provides technical support for large-scale seedling production and artificial planting and utilization of the ficus hirta, and has good economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency tissue culture and breeding method of Ficus phalaenopsis. Background technique [0002] Ficus hirta Vahl, also known as five-finger hair peach, five-finger milk, Tubeiqi, milk wood, five-clawed dragon, etc., is a perennial shrub or small deciduous tree of the genus Ficus in the family Moraceae, and the whole plant is densely covered with coarse hairs , the branches and leaves contain latex, and the root bark has aroma. It grows naturally on hillsides, wastelands, valleys or bushes. It is mainly distributed in the southeast and southwest provinces of Jiangxi, Guangxi, Fujian, Hainan, Malaysia, Myanmar, and Vietnam. , Thailand and other Southeast Asian countries. The root of Ficus vulgaris can be used as medicine. It tastes sweet, pungent, and flat in nature. It is beneficial to qi and spleen, promotes qi and relieves depression, relaxes ...

AI Technical Summary

Problems solved by technology

[0004] The plants of Ficus syringae are pulpy and have many bristles on the body surface, and it is difficult to induce callus and adventitious buds, and it is difficult to obtain tissue culture seedlings

At present, there are very limited reports on the tissue culture of Ficus syringae, which are only in the exploratory stage. Jiang Lin et al. used the stem nodes of Ficus syringae as explants for in vitro regeneration research, and Huang Sai et al. used the terminal buds as explants for bud proliferation and culture. In the research, Su Yuqin used the explants of the Ficus thick-leaf stem tip to conduct the primary culture, subculture and rooting culture experiments. Li Linxuan and Liang Chunhui used the leaves, petioles and stems as explants to induce callus, and conducted a comparative study of different hormones. , Tao Yu et al. compared the effects of 6-BA, NAA, etc. on the callus induction rate and root incidence rate with fresh Ficus rough leaves seeds as test materials. The publication numbers are CN104106468 B, CN 104106468 A, CN 111213588 A and CN The invention patent of 112088775 A also discloses the method of tissue culture and breeding of Ficus vulgaris. Most of the above technologies adopt the indirect in vitro regeneration method for breeding, which generally has low induction rate and multiplication coefficient, long seedling cultivation period, high production cost and poor quality of seedlings. Poor problem, it is difficult to apply it for large-scale breeding of seedlings

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