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Platinum nano-enzyme/glucose oxidase(at)hyaluronic acid composite antibacterial material as well as preparation and application thereof

A technology of glucose oxidase and hyaluronic acid, applied in oxidoreductase, biochemical equipment and methods, nanotechnology, etc., can solve the problems of low activity of nanozymes, improve antibacterial activity, reduce toxic and side effects, and promote rapid healing Effect

Active Publication Date: 2021-11-05
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of low activity of nanozymes in the antibacterial process of chronic wounds, the present invention provides a platinum nanozyme / glucose oxidase@hyaluronic acid composite antibacterial material, which aims to provide a solution with good stability for diabetic chronic wounds , a new material with excellent targeting and antibacterial activity

Method used

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  • Platinum nano-enzyme/glucose oxidase(at)hyaluronic acid composite antibacterial material as well as preparation and application thereof
  • Platinum nano-enzyme/glucose oxidase(at)hyaluronic acid composite antibacterial material as well as preparation and application thereof
  • Platinum nano-enzyme/glucose oxidase(at)hyaluronic acid composite antibacterial material as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Catalytic Activity of Platinum Nanozyme at Different pH

[0058] (1) each get 1ml 0.03mg / ml platinum nanozyme (prepared by above step (2)) solution in 9 centrifuge tubes, centrifuge to remove supernatant;

[0059] (2) Add deionized water (Blank group) and 1ml of PBS buffer with pHs of 3, 4, 5, 6, 7, 8, 9, and 10 respectively;

[0060] (3) Add 1 μl of 5 mg / ml methylene blue solution and 10 μl of 100 mM hydrogen peroxide solution respectively;

[0061] (4) Incubate at 37°C for 8 hours and take out;

[0062] (5) Measure the ultraviolet absorption changes of MB (methylene blue) at 665 nm in the above 9 groups of experimental solution systems.

[0063] Result analysis: Based on the performance of MB dyes that can be degraded by OH and used as an indicator of OH formation, from figure 1 It can be seen from the UV absorption changes of MB in the medium that the platinum nanozyme catalyzes hydrogen peroxide to produce more OH under acidic conditions, showing better catalytic...

Embodiment 2

[0065] Stability study

[0066] (1) Add the PAGH nanosystem (prepared in step (3)) to deionized water (Water), saline (Saline), phosphate buffer saline (PBS), cell culture medium (Medium ) middle;

[0067] (2) At room temperature, stand for 30 days respectively;

[0068] (3) figure 2 (A): Observe and take pictures to record the dispersion of PAGH on the 1st, 5th, 10th, 15th, and 30th days in different systems, and observe whether there is precipitation;

[0069] (4) figure 2 (B): On day 1, day 5, day 10, day 15, and day 30, take PAGH in different systems to measure its particle size, and monitor the change of particle size.

[0070] Analysis of results: from figure 2 (A) It can be seen that from the 1st day to the 30th day, in deionized water (Water), saline (Saline), phosphate buffered saline (PBS), cell culture medium (Medium) containing 10% fetal bovine serum ) in four different systems, PAGH can be stably dispersed, no precipitation is observed; from figure 2 (B...

Embodiment 3

[0072] (1) Take 0.03mg / ml PAGH and dissolve in pH=8 PBS buffer solution containing 10mM glucose;

[0073] (2) Add 100U / ml HAase solution to the above system, mix well, and react at room temperature;

[0074] (3) measure the pH value of the system with a pH meter at the 0th minute, the 30th minute, the 60th minute, the 90th minute, the 120th minute, the 150th minute, and the 180th minute after the reaction starts and record it;

[0075] (4) A pH=8 PBS buffer solution containing 10 mM glucose was used as a blank control group.

[0076] Analysis of results: from image 3 It can be seen that after PAGH is activated and degraded by HAase, the pH value of the system decreases, and the solution changes from alkaline to acidic, while the control group remains alkaline. This shows that after PAGH is activated by HAase, the released GOX can catalyze the production of gluconic acid from glucose and reduce the pH of the solution.

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Abstract

The invention belongs to the technical field of drugs for treating diabetic chronic wounds, and particularly discloses a platinum nano-enzyme / glucose oxidase(at)hyaluronic acid composite antibacterial material which comprises a core and a shell wrapping the core; the core comprises platinum nano-enzyme and glucose oxidase; and the shell is made of hyaluronic acid. The invention also provides preparation and application of the antibacterial material. Research finds that the composite material can effectively and synergistically improve the tissue environment of a diabetic wound and can efficiently and specifically kill pathogenic bacteria such as staphylococcus aureus through the innovative components and the synergism of the composite morphology of the components; moreover, the material disclosed by the invention basically does not influence metabolism of normal cells and has small toxic and side effects.

Description

technical field [0001] The invention belongs to the field of nanotechnology and targeted sterilization, and relates to an antibacterial material based on chronic wounds such as diabetic chronic wounds. Background technique [0002] Wound refers to the place where the injury ruptures. According to the different causes, it can be divided into three categories: acute trauma, surgical wound and chronic wound. The first two tend to heal spontaneously, while chronic wounds are difficult or even impossible to heal spontaneously. [0003] The most common example of a chronic wound is a diabetic wound. Diabetes is an important disease, and its incidence is increasing year by year. It is estimated that by 2030, the number of diabetic patients in the world will reach 550 million. The problem of diabetic chronic wounds is also becoming more and more prominent. Because it is difficult to heal, it has brought a heavy burden to the medical systems of countries around the world. Every yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K9/51A61K47/36A61K47/54A61P17/02A61P31/04B82Y5/00B82Y30/00B82Y40/00A61K33/243
CPCA61K47/549A61K33/243A61K9/5161A61K38/443C12Y101/03004A61P17/02A61P31/04B82Y5/00B82Y30/00B82Y40/00A61K2300/00Y02A50/30
Inventor 卿志和杨荣华邢硕晖陈丽芳付光为左琴
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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