Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polyethyleneimine derivative as well as preparation method and application thereof

A technology of polyethyleneimine and its derivatives, applied in the field of biomedicine, can solve the problems of limiting PEI, low loading efficiency, unstable loading, etc., and achieve the effect of reducing cytotoxicity and high transfection efficiency

Inactive Publication Date: 2021-11-05
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at the same time, due to the low loading efficiency of PEI on protein and nucleic acid molecules, the loading instability, and the contradiction between transfection efficiency and cytotoxicity, it greatly hinders the application of PEI and limits the further promotion of PEI into clinical applications. develop

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polyethyleneimine derivative as well as preparation method and application thereof
  • Polyethyleneimine derivative as well as preparation method and application thereof
  • Polyethyleneimine derivative as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation of hyperbranched PEI material (PEI-M) with five-membered ring and six-membered ring modification, 40 times equivalent of five-membered and six-membered nitrogen-containing heterocyclic small molecules (as shown in Table 2), 60 times equivalent of EDC Weigh HCl, 80 times the equivalent of NHS into a reaction bottle with a stirring bar, add anhydrous DMSO and stir the reaction at 35°C for 30 minutes to activate the carboxyl group on the small molecule, then add 1 equivalent of it that has been dissolved in DMSO The hyperbranched PEI-10kDa reaction was continued with stirring at 35 °C for 72 h. Subsequently, the reaction system was settled with 10 times the volume of diethyl ether, the upper layer liquid was discarded, and the residual diethyl ether was sucked dry. Then dissolve the reaction product with sterile water, dialyze in Milli Q water with a dialysis bag with a molecular weight cut-off of 7000Da, the pH value gradient increases from 3-7, change the...

Embodiment 2

[0061] The preparation of the hyperbranched PEI material (PEI-M) modified by the seven-membered ring, 48 times the equivalent of carbonyldiimidazole (CDI) was weighed into a reaction bottle with a stirring bar, dissolved in anhydrous DMSO, and 40 times the equivalent of seven Small nitrogen-containing heterocyclic molecules (as shown in Table 2), specifically N-(2-hydroxyethyl)hexamethylenediamine, were injected into the reaction vial with a syringe, and then stirred and reacted at 35°C for 12 hours to carry out the hydroxyl group reaction. For the reaction with CDI, add 1 equivalent of hyperbranched PEI-10kDa dissolved in DMSO in advance, and continue to stir and react at 35°C for 24 hours. Subsequently, the reaction system was settled with 10 times the volume of diethyl ether, the upper layer liquid was discarded, and the residual diethyl ether was sucked dry. Then dissolve the reaction product with sterile water, and dialyze in MilliQ water with a dialysis bag with a molecu...

Embodiment 3

[0064] The 28 kinds of PEI-M obtained in Example 1 were used to induce DC2.4 cells to release IFN-β. Fully dissolve PEI-M with sterile water and add it to a 96-well plate with 15,000 DC2.4 cells per well. The concentration of PEI-M is 20 μg / mL or 50 μg / mL. The volume of PEI-M-containing medium was 200 μL. After 24 hours, the medium in the well plate was collected and centrifuged at 3000 rpm for 5 minutes, the medium supernatant was collected, and the concentration of IFN-β was detected with an ELISA kit. The process of ELISA detection is (all processes are carried out at room temperature): incubate the capture antibody overnight in a high-adhesion 96-well plate, wash three times with PBST (containing 0.05% PBS solution), block with PBS containing 1% BSA for 1 hour, PBST Wash 3 times, add the sample to be detected (3-4 replicates) and Bracket sample and incubate for 2 hours, wash 3 times with PBST, add detection antibody and incubate for 2 hours, wash 3 times with PBST, add st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a polyethyleneimine derivative. The polyethyleneimine derivative is obtained by bonding PEI and nitrogen heterocyclic ring micromolecules. Compared with PEI, a PEI derivative material provided by the invention can reduce the toxicity of PEI and has the function of stimulating inherent immune response. The PEI derivative material provided by the invention can be used for loading protein or mRNA antigen through simple mixing to prepare a novel vaccine, and since a vector has an immune stimulation function, the design of the vaccine can be simplified and the response rate of the vaccine can be improved; and the PEI derivative provided by the invention can also be used for transfection vectors of mRNA and DNA, and can realize better cell transfection efficiency on the premise of reducing the toxicity of PEI.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a polyethyleneimine derivative and its preparation method and application. Background technique [0002] Biopolymer materials have always played an indispensable role in the application of anti-tumor therapy, and they have great application potential and development prospects in the design of nano-vaccine vectors and gene transfection vectors for anti-tumor therapy. However, due to the low efficiency of nano vaccines, complex components, high toxicity of gene carrier materials, and low transfection efficiency, the development of polymer materials in the clinical application of nano vaccines and gene carriers has been slow. Therefore, it is of great significance to develop new polymer materials for the design of tumor vaccine vectors and gene vectors, and to accelerate the clinical transformation of nano-vaccine and gene therapy for cancer treatment. [0003] Cationic polymer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/02A61K39/00A61K47/22A61K47/34A61K48/00A61P35/00
CPCC08G73/0206A61K47/34A61K47/22A61K39/0011A61K48/0041A61P35/00
Inventor 宋万通赵佳雨徐玉笛高雨茜汤朝晖陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com