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Microbial aerosol sampling bottle

A sampling bottle and aerosol technology, which is applied in the methods of sampling biological materials, enzymology/microbiology devices, and methods of supporting/immobilizing microorganisms, etc., can solve the problem of insufficient sampling effect, secondary aerosol escape, and reduced sampling effect and other issues, to achieve the effect of reducing sample cross-contamination, reducing escape loss, and improving sampling efficiency

Pending Publication Date: 2021-11-05
JIANGSU MOLE BIOSCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing wet wall cyclone type microbial aerosol collection device, the air path residues on the cyclone wall in the air inlet equipment are likely to cause cross contamination and affect the sampling results; and the existing wet wall cyclone type air The sampling efficiency of the aerosol collection device is low, and the sampling effect cannot be achieved when the cyclone speed is slow, and the rapid cyclone speed will cause secondary aerosol escape, which greatly reduces the sampling effect and limits the application of the wet wall cyclone method. A microbial aerosol sampling bottle is used, so that the cyclone air intake duct is all located in the sampling bottle, which avoids the pollution of the sampling liquid due to the residual liquid of the air intake equipment; and a cyclone filter is set in the sampling bottle, which increases the humidity of the cyclone. The wall area effectively reduces the escape of secondary aerosols and improves the sampling efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The mesh number of the conical filter is 100 mesh, the diameter of the top surface is 26mm, and the angle between the hypotenuse line and the center line is 15°; the inner diameter of the cylindrical part of the air inlet part and the collection part of the sampling bottle is 44mm ; The height of the cylindrical part of the air inlet and the collection part is 30mm and 42mm respectively; the area of ​​the air inlet on the air inlet is 220mm 2 .

Embodiment 2

[0043] The mesh number of the conical filter is 60 mesh, the diameter of the top surface is 21mm, and the angle between the hypotenuse line and the center line is 12°; the inner diameter of the cylindrical part of the air inlet part and the collection part of the sampling bottle is 39mm ; The height of the cylindrical part of the air inlet and the collection part is 25mm and 38mm respectively; the area of ​​the air inlet on the air inlet is 180mm 2 .

Embodiment 3

[0045] The mesh number of the conical filter is 40 mesh, the diameter of the top surface is 16mm, and the angle between the hypotenuse line and the center line is 10°; the inner diameter of the cylindrical part of the air inlet part and the collection part of the sampling bottle is 34mm The heights of the cylindrical parts of the air inlet and the collection are 20mm and 33mm respectively; the area of ​​the air inlet on the air inlet is 162mm 2 .

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Abstract

The invention discloses a microbial aerosol sampling bottle. The microbial aerosol sampling bottle comprises a sampling bottle body and a sampling bottle cap, wherein the sampling bottle body comprises an air inlet part, a collecting part and a placing part, a threaded connection part used for being connected with the sampling bottle cap is arranged at the outer side of the bottle body between the air inlet part and the collecting part, and the sampling bottle cap sleeves the outer side of the air inlet part and is in threaded connection with the threaded connection part; and an air inlet is formed in the side wall of the air inlet part, a filter screen is arranged in the sampling bottle body, the filter screen is fixed to the top of the air inlet part through a filter screen fixing ring, the filter screen fixing ring comprises a fixing part and an air outlet pipe located below the fixing part, a connecting hole communicating with the air outlet pipe is formed in the fixing part, and the bottom of the air outlet pipe is connected with the filter screen. According to the microbial aerosol sampling bottle, a cyclone air inlet duct is completely located in the sampling bottle, so that the pollution to sampled liquid caused by residual liquid of air inlet equipment is avoided; and a cyclone filter screen is arranged in the sampling bottle, so that the wetted wall area during cyclone is increased, the escape of secondary aerosol is effectively reduced, and the sampling efficiency is increased.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring devices, in particular to a microbial aerosol sampling bottle. Background technique [0002] Atmospheric aerosol refers to a stable mixed system composed of solid particles and liquid particles uniformly dispersed in the atmosphere, and the particles are collectively referred to as aerosol particles. Among them, biologically active microbial substances or particles are collectively referred to as microbial aerosol particles, including microorganisms such as viruses, bacteria, fungi, pollen, and spores. The aerodynamic diameter of atmospheric aerosol particles is mostly between 0.001 and 100 μm, which is very light enough to be suspended in the air. Particles with a particle size of 5-10 μm can enter the human trachea and bronchi, and particles with a particle size of less than 5 μm can penetrate deep into the deep respiratory system. When the concentration of aerosol reaches a h...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/24C12M1/12C12M1/00C03C17/34
CPCC12M23/08C12M23/20C12M23/38C12M23/46C12M23/48C12M33/14C12M33/22C03C17/3405C03C2217/78C03C2218/111C03C2218/32
Inventor 李杨霞何竹理崔慧辉贾俊玲曾丽
Owner JIANGSU MOLE BIOSCI
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