Mycobacterium tuberculosis multi-line drug resistance gene identification method and device

Abstract

The invention relates to a mycobacterium tuberculosis multi-line drug resistance gene identification method and device. The method comprises the following steps: acquiring a whole genome sequence obtained by metagenome sequencing of a sample or a sequence of a mycobacterium tuberculosis drug-resistant target region obtained on the basis of targeted amplification sequencing, and carrying out data quality control; comparing the sequence subjected to the data quality control to a mycobacterium tuberculosis genome, sequencing and screening out a comparison result meeting the quality requirement; carrying out local assembly and variation detection on the sequences of the gene regions where the variation sites of which the comparison positions are located in the mycobacterium tuberculosis drug resistance gene database are located; annotating a variation detection result to the mycobacterium tuberculosis drug resistance gene database according to the positions and mutation types of variation sites on the genome; and outputting a detection result of each drug resistance related mutation sites in the mycobacterium tuberculosis drug resistance gene database based on an annotation result. According to the invention, multiple drugs and corresponding drug resistance sites can be covered in one detection, and the detection precision is high.

Description

technical field [0001] The invention relates to the technical field of mycobacterium tuberculosis detection, in particular to a method and a device for identifying multiline drug resistance genes of mycobacterium tuberculosis. Background technique [0002] At present, the methods for drug susceptibility detection of Mycobacterium tuberculosis on the market are mainly divided into drug susceptibility detection based on strain culture and fluorescence quantitative PCR method based on molecular biology. [0003] For drug susceptibility testing based on bacterial strain culture, Mycobacterium tuberculosis is spread on a culture plate, and after Mycobacterium tuberculosis covers the entire plate, different doses of drugs are placed on the plate. After continuing to cultivate for a period of time, observe the size of plaques around the drug, so as to judge the tolerance of the bacteria. This technology needs to be carried out in a stricter biosafety laboratory, and it will take 2...

AI Technical Summary

Problems solved by technology

This method only takes half a day to one day, but currently there are only detection kits for common mutation sites of first-line drugs on the market. In addition, this method cannot detect frameshift mutations or mutations without mutation hotspots

[0005] At present, there is no Mycobacterium tuberculosis drug resistance site detection product based on whole genome sequencing on the market, nor is there any next-generation sequencing-based Mycobacterium tuberculosis mutation detection and analysis software

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