Efficient extraction method of inonotus obliquus total triterpenes and preparation method of fine powder of inonotus obliquus total triterpenes

Abstract

The invention relates to an efficient extraction method of inonotus obliquus total triterpenes and a preparation method of fine powder of the inonotus obliquus total triterpenes. The method comprises the following steps: crushing an inonotus obliquus raw material to obtain inonotus obliquus coarse powder; grinding the inonotus obliquus coarse powder in a liquid-nitrogen low-temperature environment by using a ball mill to obtain inonotus obliquus micropowder; preparing an inonotus obliquus suspension from the inonotus obliquus micropowder; carrying out shearing ultrafine crushing treatment on the inonotus obliquus suspension to obtain an inonotus obliquus ultrafine powder suspension; centrifuging the inonotus obliquus ultrafine powder suspension to obtain a precipitate, and drying the precipitate to obtain inonotus obliquus ultrafine powder; placing the inonotus obliquus ultrafine powder in a steam explosion test device to obtain ultrafine wall-broken powder; adding ethanol into the ultrafine wall-broken powder, and carrying out vacuum combined ultrasonic microwave-assisted extraction to obtain an inonotus obliquus total-triterpene crude extract; and carrying out vacuum concentration to obtain an inonotus obliquus total-triterpene concentrated lixiviate. According to the method, the ethanol dosage and the extraction time are reduced under the same extraction rate, the purity of the obtained triterpenes is high, and the biological activity of the inonotus obliquus total triterpenes is high.

Description

1. Technical field: [0001] The invention relates to the technical field of extraction of functional components of Inonotus obliquus, in particular to a method for efficiently extracting total triterpenes of Inonotus obliquus and a method for preparing fine powder thereof. 2. Background technology: [0002] Inonotus obliquus ( Inonotus obliquus (Fr.) Pilat) is also known as chaga, oblique tube microporus, etc. It is mainly distributed in the northern hemisphere at 40-50° north latitude, such as Russia, Japan, and Finland. In my country, it is mainly distributed in the Changbai Mountain area of ​​Jilin Province, Heilongjiang The Greater and Lesser Khingan Mountains and other areas have great regional characteristics. At present, researchers in the United States, Japan, and South Korea are increasing their research efforts on Inonotus obliquus, especially in Russia, where wild Inonotus obliquus fruiting bodies are used by folks to treat various diseases. Inonotus obliquus has ...

AI Technical Summary

Problems solved by technology

Although triterpenoids have good functional properties, their application is limited because they are mainly dissolved in organic solvents such as ethanol and are heat sensitive.

However, this method has problems such as high production cost and small processing capacity.

[0007] The triterpenoids prepared in the above patents were processed by different processing technologies such as ultrasonic-assisted ethanol extraction, quorum sensing molecular induction, and dynamic high-pressure micro-jet. Certain defects, such as large solvent demand, long extraction time, many uncontrollable factors, small processing capacity, poor biological activity of the product, etc.

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