Single cell detection method and system for detecting early lung cancer

Abstract

The invention relates to the field of lung cancer detection methods, in particular to a single cell detection method and system for detecting early lung cancer. The detection method adopts a tip-enhanced Raman system to detect lung cancer cells, wherein the tip-enhanced Raman system sequentially comprises a metal substrate and a metal probe from bottom to top, only a single metal atom is arranged at the top end of the tip of the metal probe to form a nano resonant cavity, the volume of the nano resonant cavity is within 1 nm<3> to localizeincident light within a certain range so as to improve the spatial resolution, the focused incident laser irradiates the metal probe, and when the tip of the metal probe is close to the metal substrate, space local plasmon is generated in a gap between the tip and the substrate, so that a Raman scattering image with high spatial resolution is generated. According to the present invention, the cancer cell aggregation condition can be accurately detected so as to reveal the difference between the cancerous tissue and the normal cell tissue structure on the molecular level, the Raman spectrum of the cancerous tissue and the Raman spectrum of the normal tissue are compared, the characteristic spectrum capable of reflecting the tissue lesion information can be found according to the difference, and the early lung cancer cell aggregation condition can be found.

Description

technical field [0001] The invention relates to the field of lung cancer detection methods, in particular to a single-cell detection method and detection system for detecting early lung cancer. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Lung cancer is difficult to be found by patients in the early stage, so it always leads to missing the best treatment period. Lung cancer detection methods in the prior art include: sputum exfoliation cytology examination, fiberoptic bronchoscopy, CT examination, magnetic resonance imaging, mediastinoscopy and thoracotomy exploration; if through sputum cytology examination, bronchoscopy If both examination and needle biopsy fail to e...

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Problems solved by technology

[0007] The inventors found that Raman spectroscopy imaging at the single-molecule level requires extremely harsh experimental environments and technical means, which hinders the development of molecular Raman imaging. The Raman spectroscopy technology in the prior art cannot realize the molecular level of lung cancer detection

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