Electrochemical analysis method based on tetrahedral DNA nanoprobe and application

Abstract

The invention belongs to the technical field of DNA methylation analysis, and discloses an electrochemical analysis method based on a tetrahedral DNA nanoprobe and application, and the electrochemical analysis method comprises a tetrahedral probe structure sequence, different DNA methylation target sequences, a signal amplification sequence and a comparison sequence. According to the present invention, the optimal performance is represented under the conditions of the tetrahedral probe fixation time of 3 h, the target sequence hybridization time of 1 h and the HpaII incision enzyme digestion time of 2 h, the good detection ability is represented within the range of 1 amol / L to 1 pmol / L, the detection limit reaches 0.93 amol / L, and the sensitivity is high; the electrochemical biosensing technology has the unique advantages in the aspect of DNA methylation detection: specific analysis on a methylated DNA target sequence can be completed within a short time, the operation is convenient, the price is low, the sensitivity is high, the specificity is good, the stability is strong, and the electrochemical biosensing technology can be effectively used for DNA methylation detection in complex systems such as serum.

Description

technical field [0001] The invention belongs to the technical field of DNA methylation biosensing analysis, and in particular relates to an electrochemical analysis method and application based on a tetrahedral DNA nanoprobe. Background technique [0002] At present: DNA methylation plays an important role in the process of gene transcription. Once abnormal, it can lead to the occurrence and development of Alzheimer's disease, cardiovascular and cerebrovascular diseases, and malignant tumors. At present, there are many DNA methylation analysis methods, such as methylation-specific polymerase chain reaction, fluorescence quantification, methylation-specific high-resolution melting curve, pyrosequencing and other methods based on bisulfite conversion, Analysis methods based on methylation-specific recognition molecules such as methylation restriction endonucleases, methyl-binding domain proteins, anti-5-methylcytosine antibodies, and zinc finger proteins, as well as high-perfo...

AI Technical Summary

Problems solved by technology

[0003] Through the above analysis, the problems and defects of the existing technology are: the traditional DNA methylation analysis method is cumbersome to operate, poor in stability, and poor in specificity, which will lead to the degradation of the DNA to be tested, and requires specific large-scale equipment, experimental sites and professional data analysis technology personnel, clinical application is relatively limited

[0004] The difficulty and significance of solving the above problems and defects: With the development of medicine, the role of DNA methylation in the occurrence and development of diseases has become increasingly prominent, and it plays an important role in the "early detection, early diagnosis, and early treatment" of diseases. Traditional analysis methods are difficult to meet the current clinical detection needs of DNA methylation

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