Acetylcysteine-stabilized gold nanocluster for acute kidney injury as well as preparation method and application thereof

A technology for acetylcysteine ​​and acute kidney injury, applied in the field of biomedical materials, can solve the problems of limited curative effect, low utilization rate of small molecule drugs, large toxic and side effects, etc., and achieve good therapeutic effect, excellent biocompatibility and Biosecurity Effects

Active Publication Date: 2021-11-19
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, small molecule drugs have low utilization, high toxicity and limited efficacy
These hinder their clinical application

Method used

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  • Acetylcysteine-stabilized gold nanocluster for acute kidney injury as well as preparation method and application thereof
  • Acetylcysteine-stabilized gold nanocluster for acute kidney injury as well as preparation method and application thereof
  • Acetylcysteine-stabilized gold nanocluster for acute kidney injury as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment A

[0034] In the embodiment of the present invention, Au NCs-NAC includes surface ligand acetylcysteine ​​and gold nanoclusters protected by the surface ligand acetylcysteine, and the gap between the gold nanocluster and the acetylcysteine ​​is Through the gold-sulfur coordination bond, specifically, the gold element on the surface of the gold nanocluster forms a coordination bond with the sulfur element in the acetylcysteine, so that the acetylcysteine ​​is bound to the gold nanocluster surface. The acetylcysteine ​​can effectively stabilize the gold nanoclusters and control the ultrasmall size of the gold nanoclusters, so that the finally obtained Au NCs-NAC has an ultrasmall size. Moreover, they all have good water solubility and biological safety, are not easy to interact with serum proteins, and are beneficial to the circulation of Au NCs-NAC in the blood. In the embodiment of the present invention, the Au NCs-NAC has an ultra-small size, which is beneficial to effectively ...

Embodiment 1

[0048] Example 1: Synthesis of Au NCs-NAC

[0049] Au NCs-NAC synthesis: Add chloroauric acid (2 mL at a concentration of 20 mg / mL) and aqueous NaOH (3 mL at a concentration of 0.5 mol / mL) to an aqueous NAC solution (20 mL at a concentration of 80 mmol / mL ) and stirred at 37°C for 2.5 hours. Then, the reacted aqueous solution was dialyzed for two days, and the water was changed every 4 hours. Finally, the resulting solution was stored in a refrigerator at 4°C for future use.

[0050] figure 1 is a roadmap for the synthesis of Au NCs-NAC, where HAuCl 4 stands for chloroauric acid and NAC stands for acetylcysteine. The surface ligand acetylcysteine ​​in the Au NCs-NAC can well stabilize the gold nanoclusters.

[0051] figure 2 is the TEM image of the synthesized Au NCs-NAC; image 3 is the XPS figure of the synthesized Au NCs-NAC; figure 2 and image 3 It indicated the successful synthesis of Au NCs-NAC with ultra-small size, and the S element in XPS indicated the suc...

Embodiment 2

[0052] Example 2: The ability of Au NCs-NAC to scavenge various active oxygen and the ability of Au NCs-NAC to scavenge hydroxyl radicals

[0053] The hydroxyl radical scavenging efficiency of different concentrations of Au NCs-NAC (25-100 μg / mL) was determined by the hydroxyl radical antioxidant capacity (HORAC) kit (Cell Biolabs, Inc., USA). Testing was performed according to the protocol provided by the manufacturer.

[0054] Such as Figure 4 As shown, Au NCs-NAC can effectively scavenge hydroxyl radicals in a concentration-dependent manner. Moreover, the clearance efficiency of Au NCs-NAC was significantly better than that of acetylcysteine ​​alone. Under the condition of 100μg / mL concentration, Au NCs-NAC can remove 77.7% of hydroxyl radicals, while acetylcysteine ​​can only remove 47.2%.

[0055] The superoxide anion scavenging efficiency of different concentrations of Au NCs-NAC (25-100 μg / mL) was determined by SOD detection kit (Sigma-Aldrich, USA). Testing was pe...

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Abstract

The invention discloses an acetylcysteine-stabilized gold nanocluster for acute kidney injury as well as a preparation method and application of the acetylcysteine-stabilized gold nanocluster. The acetylcysteine-stabilized gold nanocluster (Au NCs-NAC) comprises a gold nanocluster and acetylcysteine combined on the surface of the gold nanocluster. The Au NCs-NAC disclosed by the invention comprises acetylcysteine serving as a surface ligand and the gold nanocluster protected by the surface ligand. The Au NCs-NAC designed by the invention has an ultra-small size, can be effectively enriched in the kidney of a mouse, can relieve and treat glycerol-induced acute kidney injury by removing a large amount of active oxygen or active nitrogen in a renal tubule, has excellent anti-inflammatory ability, and has a better treatment effect than acetylcysteine. In addition, the Au NCs-NAC has excellent biocompatibility and excellent biological safety.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an acetylcysteine-stabilized gold nanocluster (referred to as Au NCs-NAC) for acute kidney injury and its preparation method and application. Background technique [0002] Acute kidney injury is an important health problem in humans. Due to its high morbidity and mortality, it is estimated that 1.7 million people die every year worldwide. Currently, adjuvant therapy and kidney transplantation are the most common treatments. Recent studies have shown that the pathogenesis of acute kidney injury is associated with excess intracellular reactive oxygen and nitrogen species. Previously, some small-molecule drugs, such as amifostine and acetylcysteine, have been shown to act as antioxidants and eliminate reactive oxygen species to alleviate acute kidney injury. However, small molecule drugs have low utilization rate, high toxicity and limited efficacy. These hinder the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K33/242A61P13/12B82Y5/00B82Y30/00
CPCA61K33/242A61K47/542A61P13/12B82Y5/00B82Y30/00Y02A50/30
Inventor 黄鹏涂天慧张东阳林静
Owner SHENZHEN UNIV
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