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Preparation method of protein raw material rich in vitamin K2 and functional peptide

A protein raw material and vitamin technology, applied in the field of feed protein, can solve the problems of loss of functional peptides, poor product temperature, fluctuation of enzymatic hydrolysis products, etc., and achieve the effect of reducing loss, reducing emissions and realizing comprehensive utilization.

Inactive Publication Date: 2021-11-26
唐波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this invention, soybeans are enzymatically hydrolyzed and subjected to microbial fermentation. During the fermentation process, microorganisms will use part of the peptides produced by soybean enzymolysis, resulting in the loss of functional peptides. When fermenting after enzymatic hydrolysis, microorganisms are affected by various factors. The enzymatic hydrolysis product will fluctuate greatly, and the temperature of the product is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] a: Preparation of liquid fermentation medium

[0030] 1) Seed medium preparation: 40g / L glucose, 35g / L sucrose, 50g / L peptone, 2.8g / L potassium chloride, 5g / L yeast extract, constant volume with tap water, natural pH, sterilized at 120°C for 15 minutes ;

[0031] 2) Preparation of fermentation medium: 50g / L glucose, 2g / L sucrose, 20g / L glycerin, 30g / L peptone, 10g / L yeast extract, 1g / L dipotassium hydrogen phosphate, constant volume with tap water, natural pH, Sterilize at 118°C for 20 minutes, sterilize the glucose solution separately, and then add it to the fermentation medium according to the above addition ratio;

[0032] b: liquid fermentation

[0033] 1) Bacillus subtilis natto (CICC 10023) was inoculated into a shake flask containing a seed medium, the volume of liquid in the medicine bottle was 10%, the culture temperature was 38°C, the rotation speed of the shake flask was 200r / min, and fermentation was carried out for 18 hours to obtain a seed liquid;

[00...

Embodiment 2

[0041] a: Preparation of liquid fermentation medium

[0042] 1) Preparation of seed medium: 50g / L glucose, 30 g / L sucrose, 30g / L peptone, 2.9g / L potassium chloride, 5g / L yeast extract, constant volume with tap water, natural pH, sterilized at 120°C for 15 minute;

[0043] 2) Preparation of fermentation medium: 45g / L glucose, 10 g / L sucrose, 15 g / L glycerin, 30g / L peptone, 12g / L yeast extract, 0.5g / L dipotassium hydrogen phosphate, constant volume with tap water, pH Naturally, sterilize at 120°C for 15 minutes, and add the glucose solution to the medium according to the proportion after single elimination;

[0044] b: liquid fermentation

[0045] 1) Bacillus subtilis natto (CICC 10023) was inoculated into a shake flask containing a seed medium, the volume of liquid in the medicine bottle was 20%, the culture temperature was 40°C, the rotation speed of the shake flask was 200r / min, and fermentation was carried out for 16 hours to obtain the seed liquid;

[0046] 2) Inoculate ...

Embodiment 3

[0053] a: Preparation of liquid fermentation medium

[0054] 1) Seed medium preparation: 32g / L glucose, 39 g / L sucrose, 30g / L peptone, 3g / L potassium chloride, 4.9g / L yeast extract, constant volume with tap water, natural pH, sterilized at 118°C for 30 minute;

[0055] 2) Preparation of fermentation medium: 60g / L glucose, 0 g / L sucrose, 20g / L glycerin, 40g / L peptone, 8g / L yeast extract, 0.8g / L dipotassium hydrogen phosphate, constant volume with tap water, natural pH , sterilized at 118°C for 30 minutes, and the glucose solution was added to the medium according to the proportion after single elimination;

[0056] b: liquid fermentation

[0057] 1) Bacillus subtilis natto (CICC 10023) was inoculated into a shake flask containing a seed medium, the volume of liquid in the medicine bottle was 15%, the culture temperature was 39°C, the rotation speed of the shake flask was 200r / min, and fermentation was carried out for 12 hours to obtain a seed liquid;

[0058] 2) Inoculate th...

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PUM

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Abstract

The invention discloses a preparation method of a protein raw material rich in vitamin K2 and functional peptide, which comprises the following steps: a, preparation of a liquid fermentation culture medium: 1) preparation of a seed culture medium and 2) preparation of a fermentation culture medium; b, liquid state fermentation: 1) inoculating bacillus subtilis natto into a shake flask filled with a seed culture medium to obtain a seed solution; (2) inoculating the seed solution into the fermentation culture medium, and carrying out fermentation culture; c, preparing an enzymatic hydrolysate; and d, preparing the enzymatic hydrolysate; The method has the advantages that: 1, the loss of the vitamin K2 in the purification process is reduced, and the yield is high; 2, the fermentation liquor is used as an enzymatic hydrolysis system for enzymatic hydrolysis of vital wheat gluten, so that the discharge of wastewater is reduced, the comprehensive utilization of the wastewater and waste residues is realized, and the production cost is reduced; 3, embedding of the vitamin K2 in the fermentation liquor is realized in the enzymatic hydrolysis process of the vital wheat gluten, and the activity of the vitamin K2 is ensured not to be influenced; and 4, the compounding process of functional ingredients is realized, and the obtained product is rich in the vitamin K2 and the functional peptide and is a functional compound product.

Description

technical field [0001] The invention relates to the field of protein for feed, in particular to a method for preparing protein raw materials rich in vitamin K2 and functional peptides. Background technique [0002] Vitamin K2, also known as menaquinone, is an important class of fat-soluble vitamins. Because of its extremely low content in natural foods, it is known as the "platinum vitamin". It has a remarkable effect in the prevention and treatment of osteoporosis. It is called "the fourth generation anti-osteoporosis product", and its market value is equivalent to that of vitamin D3. Vitamin D3 has been widely used in the breeding industry. For example, adding vitamin D3 to chicken feed can greatly improve the absorption of minerals in the feed, thereby improving feed utilization, increasing the growth rate of broilers, strengthening bone mineralization, and reducing "weak leg disease" and hypocalcemia. However, vitamin D3 only promotes vitamin D3 to promote the convers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23K20/147A23K20/174
Inventor 唐波
Owner 唐波