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Protective solution containing combined antibiotics for dental pulp stem cell sampling

A technology of dental pulp stem cells and antibiotics, applied in the field of dental pulp stem cells, can solve the problems of resistance to penicillin and streptomycin, and achieve the effect of avoiding mold resistance

Pending Publication Date: 2021-11-26
上海赐道生命科学有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The purpose of the present invention is to overcome the deficiencies of the prior art and solve the technical problem of penicillin and streptomycin drug resistance in stem cell culture

Method used

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  • Protective solution containing combined antibiotics for dental pulp stem cell sampling
  • Protective solution containing combined antibiotics for dental pulp stem cell sampling

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] Example 1: Preparation of 9 Antibiotic Dental Pulp Stem Cell Maintenance Solution

[0027] 1. Purchasing antibiotic intermediate powder from Henan Kanghui Biological Company, a pharmaceutical company, dissolving and storing the mother liquor according to their respective dissolution characteristics.

[0028] 2. Quantitative combination according to grouping, endosperm basic maintenance solution. The basic maintenance solution uses a mature clinical-grade serum-free and xeno-protein-free mesenchymal stem cell medium (Chongqing Huaya Biological Company) currently used in the market. Each ml is dispensed into a 16 ml EP tube. Freeze in ordinary freezer.

example 2

[0030] Collect tooth samples Collect the teeth and send them to the dental hospital where the teeth were collected in a freezer. After tooth extraction, the dentist puts the pre-thawed antibiotic maintenance solution, and the four-degree ice box is sent to the GMP clean room where the stem cell ultra-clean station is located. Fluid from children's teeth can be collected.

example 3

[0031] Example 3: Isolation and culture of dental pulp stem cells

[0032] 1. Wash five times with the same maintenance solution in the ultra-clean bench, and replace the clean containers respectively;

[0033] 2. Extract the pulp tissue with the pulp-pulling needle, and digest the tissue in a 37-degree incubator for 4 hours with the double-enzyme method, shaking it several times during the period.

[0034] 3. Collect the digested cells, centrifuge and wash twice with maintenance solution, 1389 rpm (516g) for 10 minutes, discard the supernatant and keep the precipitated cells.

[0035] 4. Cells are cultured with mesenchymal stem cell medium for conventional culture. The next day, observe under the microscope whether there are mold hyphae or bacterial cells. The supernatant was collected on the 14th day to measure microbial 16S primer PCR, and a positive result indicated that antibiotics had failed. The stem cells were digested with 0.125% recombinant human trypsin, the prec...

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Abstract

The invention relates to the technical field of dental pulp stem cells, in particular to a protective solution containing combined antibiotics for dental pulp stem cell sampling, which is characterized by comprising compound sulfafurazole, vancomycin, metronidazole, rifampicin, streptomycin, gentamicin, levofloxacin, amphotericin and diflucan. The content of the antibiotics is respectively as follows: 1 g / ML of compound sulfafurazole, 2 g / ML of vancomycin, 1 g / ML of metronidazole, 0.5 mu g / ml of rifampicin, 0.1 g / ML of streptomycin, 0.1 g / ML of gentamicin, 0.1 g / ML of levofloxacin, 0.1 g / ML of amphotericinand 100 g / ML of diflucan. Compared with the prior art, the protective solution has the advantages that the protective solution is a multi-combination effective antibiotic combination product, is suitable for collection, transportation and maintenance of tooth specimens. The protective solution is a combined antibiotic product of dental multi-connected antibiotic which can cover a plurality of microorganisms.

Description

technical field [0001] The invention relates to the technical field of dental pulp stem cells, in particular to a protective solution containing combined antibiotics for sampling dental pulp stem cells. Background technique [0002] First, the source of dental pulp stem cells is abundant and has no ethical controversy. Second, it has low immunogenicity. Furthermore, it also has the function of immune regulation, and can intervene in diseases through immune regulation. The advantages of dental pulp stem cells determine that it has very considerable clinical application prospects and medical value. The results of the study show that dental pulp stem cells play a good role in clinical trials of periodontitis and other diseases. In the clinical application of oral diseases, the regeneration of pulp and dentin complex can be achieved through induction, and it has broad application prospects in the treatment of various pulp and periapical diseases, periodontal diseases, tooth r...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0664
Inventor 张良平施炜星唐声峰
Owner 上海赐道生命科学有限公司
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