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A kind of tissue culture rapid propagation method of Pinus huangshan

A technology of tissue culture rapid propagation and Huangshan pine, which is applied in the field of plant tissue culture, achieves the effects of low cultivation cost, good market and industrial application prospects, and reduced poor afforestation effect

Active Publication Date: 2022-05-13
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the conservation of Pinus huangshan germplasm resources and the rapid propagation system of tissue culture

Method used

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  • A kind of tissue culture rapid propagation method of Pinus huangshan
  • A kind of tissue culture rapid propagation method of Pinus huangshan
  • A kind of tissue culture rapid propagation method of Pinus huangshan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of tissue culture rapid propagation method of Huangshan pine of the present embodiment, comprises the following steps:

[0046] (1) Pretreatment of explants:

[0047] Huangshan pine cones collected from Huangshan Scenic Area in Anhui Province were dried under natural light to obtain Huangshan pine seeds; then, the outer testa of the Huangshan pine seeds were removed and placed in a 4°C refrigerator for later use.

[0048] (2) Disinfection of explants:

[0049] Take out the Huangshan pine seeds with the seed coat removed from the refrigerator; then, in the ultra-clean workbench, first wash the seeds with sterile water for 3 times, then use 75% alcohol to rinse for 60 seconds, use 1% sodium hypochlorite solution to soak for 10 minutes, and finally use Rinse with sterile water for 3 times; then, dry the water on sterile filter paper, and pick out the seed embryos with a scalpel to obtain the sterile explants required for inoculation.

[0050] (3) Callus induction:...

Embodiment 2

[0062] A kind of tissue culture rapid propagation method of Huangshan pine of the present embodiment, comprises the following steps:

[0063] (1) Pretreatment of explants:

[0064] Huangshan pine cones collected from Huangshan Scenic Area in Anhui Province were dried under natural light to obtain Huangshan pine seeds; then, the outer testa of the Huangshan pine seeds were removed and placed in a 4°C refrigerator for later use.

[0065] (2) Disinfection of explants:

[0066] Take out the Huangshan pine seeds with the seed coat removed from the refrigerator; then, in the ultra-clean workbench, wash the seeds with sterile water for 5 times, then rinse with 75% alcohol for 60 seconds, soak in 1% sodium hypochlorite solution for 10 minutes, and finally use Rinse with sterile water for 5 times; then, dry the water on sterile filter paper, and use a scalpel to pick out the seed embryos to obtain the sterile explants required for inoculation.

[0067] (3) Callus induction:

[0068]...

Embodiment 3

[0079] A kind of tissue culture rapid propagation method of Huangshan pine of the present embodiment, comprises the following steps:

[0080] (1) Pretreatment of explants:

[0081] The Huangshan pine cones collected from the Huangshan Scenic Area in Anhui were placed in the air under natural light to obtain the Huangshan pine seeds; then, the outer testa of the Huangshan pine seeds (such as figure 1 shown), and placed in a 4°C refrigerator for later use.

[0082] (2) Disinfection of explants:

[0083] Take out the Huangshan pine seeds with the seed coat removed from the refrigerator; then, in the ultra-clean workbench, first wash the seeds with sterile water 4 times, then use 75% alcohol to rinse for 60 seconds, use 1% sodium hypochlorite solution to soak for 10 minutes, and finally use Rinse with sterile water for 4 times; then blot the water on sterile filter paper, and pick out the seed embryos with a scalpel to obtain the sterile explants required for inoculation.

[00...

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Abstract

The invention provides a method for tissue culture and rapid propagation of Pinus Huangshan, the method is as follows: obtain the seeds of Pinus Huangshan; remove the outer testa; use 75% alcohol and 1% sodium hypochlorite solution to carry out combined disinfection; out of seed embryos; inoculate explants on callus induction medium for callus induction; select callus and transfer to adventitious bud induction medium for adventitious bud induction; transfer adventitious buds to elongation medium for Elongation culture of adventitious buds; select the adventitious buds of Pine huangshanensis with a height of 1-2 cm, and after removing the needles at the base of the buds, transfer them to the rooting medium for direct rooting culture. The present invention has the advantages of: using the seed embryo of Pinus huangshan seeds as experimental material for the first time, the tissue culture and rapid propagation technology of Pinus huangshanensis is established; and the operation is simple, the cultivation cost is low, the reproduction efficiency is high, and it is less affected by the natural environment, and has a good effect Market and industrial application prospects.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Pinus huangshan. Background technique [0002] Huangshan pine is a unique coniferous tree species in my country, and it is an important tree species for mountain greening, afforestation and ecological restoration in subtropical high-altitude areas in eastern China. Huangshan pine has strong ecological succession function, strong adaptability, high ecological environmental protection function and unique natural landscape effect. Pine trees are the harmful hosts of pine wood nematodes. Since the introduction of pine wood nematode into my country, as of December 31, 2020, it has spread to 726 county-level administrative districts in 18 provinces (autonomous regions and municipalities) across the country, seriously threatening the ecological security of our country. , biosecurity and economic development. Huangshan pi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 项艳兰延钢汤明霞许成芳王瑞佳严涵薇吴敏
Owner ANHUI AGRICULTURAL UNIVERSITY
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