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Medicine for treating acute radiation enteritis

A radiation enteritis, acute technology, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve problems such as no related reports, and achieve the effect of good application prospects

Pending Publication Date: 2021-11-30
AFFILIATED HOSPITAL OF JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Targeting the P2RX1 receptor may become a potential solution for the treatment of acute radiation enteritis, but there is no relevant report so far

Method used

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  • Medicine for treating acute radiation enteritis
  • Medicine for treating acute radiation enteritis
  • Medicine for treating acute radiation enteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Effect of radiotherapy on the expression of purinergic signal receptors in mouse intestines P2RXs and P2RYs

[0034] 1. Experimental method

[0035] (1) Animals and groups

[0036] Twelve SPF grade C57BL / C male mice, each weighing 24-26 g, were divided into two groups according to random number method, namely blank control group and radiotherapy group (n=6). The mice in the radiotherapy group were irradiated with X-rays at one time, with a total dose of 10Gy, to establish an acute radiation injury model. The blank control group did not receive X-ray irradiation, and the rest of the experimental conditions were the same as those of the radiotherapy group.

[0037] (2) RNA sample preparation and detection

[0038] After 2 days of radiotherapy, mice were treated with CO 2 Euthanasia was performed by asphyxiation, and the small intestine tissue was removed, and the contents of the intestine were carefully squeezed out and placed in Trizol. The small intestine...

Embodiment 2

[0052] Example 2 P2RX1 gene knockout improves the survival rate of mice after radiotherapy and inhibits acute radiation enteritis

[0053] 1. Experimental method

[0054] (1) Animals and groups

[0055] SPF-grade wild-type (WT) mice and P2RX1 knockout (P2rx1 - / - ) mice (Jiangsu Jicui Yaokang Co., Ltd.), 15 males, each weighing 24-26 g. Mice were irradiated with X-ray whole body at one time, with a total dose of 10Gy, to establish an acute radiation injury model.

[0056] (2) Survival rate analysis

[0057] The survival rate of the mice was continuously observed for 30 days after radiotherapy, and the survival conditions of the mice were recorded (n=10).

[0058] (3) HE staining

[0059] After 2 days of radiotherapy, mice were treated with CO 2 Euthanasia was performed by asphyxiation, small intestinal tissue was harvested, and intestinal contents were carefully squeezed out. Tissues were fixed with formalin fixative and embedded in paraffin. Sections were HE stained (n...

Embodiment 3

[0064] Example 3 P2RX1 Gene Knockout Inhibits Inflammatory Activation of Neutrophils in Mice After Radiotherapy

[0065] 1. Experimental method

[0066] (1) Animals and groups

[0067] SPF-grade wild-type (WT) mice and P2RX1 knockout (P2rx1 - / - ) 5 male mice, each weighing 24-26 g. Mice were irradiated with X-ray whole body at one time, with a total dose of 10Gy, to establish an acute radiation injury model.

[0068] (2) Detection of intestinal MPO (myeloperoxidase) activity

[0069] After 2 days of radiotherapy, mice were treated with CO 2 Euthanasia was performed by asphyxiation, small intestinal tissue was harvested, and intestinal contents were carefully squeezed out. The MPO activity of the small intestine tissue was detected according to the instructions of the MPO kit (Abcam, ab105136).

[0070] (3) Detection of neutrophil inflammatory factors

[0071] After 2 days of radiotherapy, mice were treated with CO 2 Euthanasia was performed by asphyxiation, peripheral ...

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PUM

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Abstract

The invention discloses a medicine for treating acute radiation enteritis. The medicine contains one or more of a P2RX1 receptor inhibitor, a P2RX1 protein inhibitor or a gene expression inhibitor of a coding gene of P2RX1. The invention provides an effective treatment method aiming at acute radiation enteritis, and P2RX1 is inhibited, so that expression increase of TNF-alpha and IL-6 inflammatory factors and neutrophil infiltration increase caused by acute radiation enteritis are reduced, and acute radiation enteritis is effectively treated. The method can be widely used for treating clinical acute radiation enteritis, has a good application prospect, and is worthy of protection and popularization.

Description

technical field [0001] The invention relates to the technical field of acute radiation enteritis, in particular to a medicine for acute radiation enteritis. Background technique [0002] Radiation therapy is one of the most important methods in modern malignant tumor synthesis. According to epidemiological statistics, about half of malignant tumor patients need to receive radiation therapy. In recent years, with the improvement of radiotherapy technology, the accuracy of tumor tissue irradiation during radiotherapy has been greatly improved, but radiation damage to normal tissues is still the main reason for dose limitation. Patients with abdominal cavity and pelvic cavity tumors are often accompanied by radiation enteritis when they receive radiation therapy. The clinical manifestations are abdominal distension, abdominal pain, diarrhea and bloody stool. In severe cases, intestinal perforation or intestinal obstruction may occur. [0003] According to the time of radiation...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K31/17A61P1/00
CPCA61K45/06A61K31/17A61P1/00Y02A50/30
Inventor 王旭陈飞胡静李荣坤苏玉婷丁延姬倩戴春华
Owner AFFILIATED HOSPITAL OF JIANGSU UNIV
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