Method for extracting effective components for cosmetics from paris polyphylla

Abstract

The invention discloses a method for extracting effective components for cosmetics from paris polyphylla. The method comprises the following steps of: a, crushing rhizomes of the paris polyphylla to obtain powder of the paris polyphylla; B, adding the powder of the paris polyphylla into pure water, adding cellulase for enzymolysis, and separating out filter residues and filtrate; c, adding ethanol into the filter residues for extraction, combining an extracting solution and the filtrate, and adding activated carbon to obtain a decolorizing solution; d, concentrating the decolorizing solution under reduced pressure until no alcohol smell exists to obtain a crude extract aqueous solution of the paris polyphylla; e, loading the crude extract aqueous solution of the paris polyphylla on a macroporous adsorption resin column, then eluting, and collecting an eluent to obtain an intermediate product A; f, loading the intermediate product A on a silica gel column, eluting, and collecting an eluent to obtain an intermediate product B; and g, loading the intermediate product B on a reversed phase column, eluting, and collecting an eluent to obtain saponin extract powder of the paris polyphylla. The method has the characteristics of improving the extraction rate and the extraction purity of the effective components.

Description

technical field [0001] The invention relates to a method for extracting an extract, in particular to a method for extracting active ingredients for cosmetics from Aesculus aescini. Background technique [0002] Aescin is a plant of the genus Pachyphysis in the family Liliaceae, and it is included in the 2015 edition of "Chinese Pharmacopoeia" as the legal base plant of Pachyphysis yunnanensis. Also known as flea rest, Chongtai grass, etc., it is named because its leaves are mostly 7 whorls on the stem, and the flowers are solitary on the whorled leaves. In my country's Sichuan, Yunnan, Guangxi, Guizhou, Jiangxi, Hunan and other places are distributed. The rhizome of Aescin has a long history of medicinal use, because its rhizome has multiple functions of detoxification, pain relief, calming convulsions, and clearing away heat. In addition, Aescin is also the main raw material of some important traditional Chinese medicines such as Yunnan Baiyao, Gongxuening, and Duoming Dan....

AI Technical Summary

Problems solved by technology

[0004] Since Aescin is a tuber plant, its cell wall is mostly composed of cellulose, and the active ingredients are often wrapped in the cell wall. Therefore, for the extraction of the active ingredients of Aesculus for cosmetics, the effective ingredients are spread from the inside to the outside. The mass resistance is relatively large, the extraction of active ingredients is difficult, and the extraction rate of total saponins can only reach 30%. As a result, most of the extracts of Aescinus aesculus currently used in cosmetics on the market are mixtures, and the purity of anti-inflammatory active ingredients is the highest. 40%, difficult to scale up for production

[0005] Therefore, the existing method for extracting the active ingredients of Aescinus aesculus for cosmetics has the problems of low extraction rate and low purity of the extracted components.

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