Application of protein in degradation of trypsin inhibitor

Abstract

The invention relates to the technical field of genetic engineering, in particular to application of protein in degradation of a trypsin inhibitor. The protein comprises an amino acid sequence as shown in SEQ ID NO. 1 and / or SEQ ID NO. 3. Functional proteins peptidase S8 and peptidase M84 derived from bacillus pumilus have higher trypsin inhibitor degradation capability, and a pichia pastoris expression system is further utilized to realize large-scale expression of the two peptidases with a trypsin inhibitor degradation effect; and the recombinase can efficiently and stably degrade the trypsin inhibitor, and can be applied to degradation of the trypsin inhibitor in soybean meal.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the application of a protein in the degradation of trypsin inhibitors. Background technique [0002] Trypsin inhibitor (KTI) belongs to the family of serine protease inhibitors and is one of the main antinutritional factors in soybean, accounting for about 2% of the total soybean meal. Studies have shown that excessive consumption of foods rich in trypsin inhibitors will reduce the digestion and absorption of nutrients by organisms, and even cause pancreatic hypertrophy or hyperplasia. At present, in addition to physical and chemical methods to inactivate trypsin inhibitors, using microorganisms to ferment bean raw materials to degrade trypsin inhibitors has also become a relatively common method. [0003] Bacillus, probably the oldest and most diverse genus in the Bacteria class, is widely used in different industries due to its fast growth rate, short fermentation ...

AI Technical Summary

Problems solved by technology

Studies have shown that excessive consumption of foods rich in trypsin inhibitors will reduce the digestion and absorption of nutrients by the organism, and even cause pancreatic hypertrophy or hyperplasia

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