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Bacillus velezensis and application thereof

A technology of Bacillus Velez and Bacillus, applied in the fields of application, bacteria, horticulture, etc., can solve problems such as the decline in the quality and yield of agricultural products, the pollution of field soil chemicals, and the safety of agricultural products, so as to reduce the rate of diseased plants, Good prevention and control effect, reduce the effect of morbidity

Active Publication Date: 2021-12-07
北京世纪阿姆斯生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned prevention and control methods are all palliatives but not the root cause, and the long-term use of chemical agents will also cause the field soil to be polluted by chemical agents, resulting in a decline in the quality and yield of agricultural products, and even the safety of agricultural products.

Method used

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  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment provides a screening method of Bacillus Velezens DPT-03.

[0052] First, sampling location

[0053] The sampling location in this embodiment is the disease soil in the peanut test field of Beijing Century Arms Biotechnology Co., Ltd.

[0054] Second, the separation, purification of strains

[0055] (1) Collect the disease soil block 20g, take back to the laboratory, and take back to the laboratory;

[0056] (2) Weigh the soil sample obtained by step (1), add a well-known soil sample to a triangular bottle containing 90 ml of sterile water, then placed the triangular bottle in a constant temperature clock of 28 ° C Culture for 30 min and obtained sample suspension;

[0057] (3) Take the sample suspension obtained by 1 ml of step (2), after dilution of 10 times, after dilution, get 10 -1 10 -2 10 -3 10 -4 10 -5 And 10 -6 Sample dilution;

[0058] (4) Take the 10 to take steps (3), respectively. -4 10 -5 And 10 -6 The sample dilution is applied to the LB solid ...

Embodiment 2

[0069] This embodiment provides a method of identifying the Bacillus Velezens DPT-03. The identification of the object is a strain DPT-03 that screens the Example 1.

[0070] First, morphological characteristics of strain DPT-03

[0071] The strain DPT-03 was seeded in the LB solid medium plate, which was cultured in a constant temperature incubator at 37 ° C for 48 h, and the colony surface was dried, opaque, milky white, an irregular or near circular, a micro-protrusion, strain DPT-03 Line picture figure 1 . Microscope observes the morphology of the bacteria, a single cell, Gram dye is positive, and there is a spore.

[0072] Second, identification of strain DPT-03

[0073] The strain DPT-03 obtained by screening Example 1 was inoculated in the LB solid medium, and after culturing overnight, the plate was taken from the plate in a 1.5 mL of the centrifuge, and 10 ul of S2 lysate was added, and it shaked Mix, standing for 20 min, then diluted 20 times, oscillating mixed, 12000 r ...

Embodiment 3

[0087] This embodiment provides a method of preparing the Bacillus Velezensis DPT-03 bacteria suspension.

[0088] Take the Bacillus Velezens DPT-03 single colonies on the LB solid medium plate 4 ° C, inoculated in LB liquid medium, cultured in a constant temperature clock of 37 ° C, 180R / min, and obtained Bacillus VelezensisdPT-03 bacteria Suspension, spare.

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Abstract

The invention relates to the technical field of biocontrol strains, and particularly discloses bacillus velezensis and application thereof. The preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) NO.20317; the invention further comprises the Bacillus velezensis or a processed product thereof; the invention further comprises a carrier and bacillus velezensis and / or a culture or a processed product thereof; the invention further comprises a microbial fertilizer including Bacillus velezensis or a microbial agent; and the invention further discloses application of the bacillus velezensis, a culture or a processed product thereof, a microbial agent or a microbial fertilizer to preparation of a composition for improving and / or preventing crop diseases. As a biocontrol strain, the bacillus velezensis provided by the invention can effectively improve and prevent crop diseases.

Description

Technical field [0001] The present application relates to the field of biocontrol strain, and more particularly, it relates to a bacillus Velez Its Applications. Background technique [0002] As one of the main crops of industries in my country, which occupies an important position in social production. But in recent years, crop diseases to affect crop yields trend of increasing year by year. [0003] Currently, the disease prevention and control measures are mainly crop rotation, deep plowing, cultivation of resistant varieties and the use of chemicals and so on. However, the above ways of combating all palliatives, and prolonged use of chemicals, but also lead to soil contamination field chemicals, resulting in decreased yield and quality of agricultural products, or even appear safety of agricultural products. [0004] Prevention and control of biological agriculture is an important direction of green development, the use of biocontrol is the prevention and control of diseases...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G13/00C12R1/07
CPCC12N1/20A01G13/00
Inventor 张志鹏彭启超黄德龙邓祖科魏浩吴妍李俊
Owner 北京世纪阿姆斯生物工程有限公司
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