Application of syringin in preparation of composition for improving skin elasticity and/or improving wrinkles
A syringin, a technology for improving skin, applied in the application field of the composition, can solve the problems of undiscovered patent publications and the like, achieve a significant protective effect, improve skin elasticity, inhibit cell apoptosis and secretion of cell inflammatory factors Effect
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[0050] The present invention also provides a preparation method of the above-mentioned composition for improving skin elasticity and improving wrinkles, which includes the following steps: mixing the components uniformly in proportion.
[0051] In a specific embodiment of the present invention, after the auxiliary material and water are uniformly mixed, they are uniformly mixed with syringin at 35-40° C., and the temperature is lowered to discharge.
[0052] The remaining materials are mixed with syringin at a relatively low temperature to avoid the impact of high temperature on the stability of the active ingredient syringin and ensure the effectiveness of the composition.
[0053] In order to further improve the uniformity of dispersion and the stability of properties of each dosage form, the present invention provides the preparation method of different dosage forms, specifically as follows:
[0054] In a specific embodiment of the present invention, the preparation method ...
experiment Embodiment 1
[0059] Research on syringin promoting collagen biosynthesis:
[0060] By using the modified DMEM medium (Dulbecco's Modified Eagle's Medium; Sigma Company) added with 10% fetal bovine serum (FBS; Hyclone Company) and 1 (w / v)% penicillin / streptomycin (Sigma Company), At 37°C, 5% CO 2 Human epithelial cells (NormalHumanDermalFibroblast (NHDF), ATCC company) were cultured in an incubator.
[0061] For the collagen synthesis experiment, the culture medium was replaced with serum-free modified DMEM medium, and treated with syringin at a concentration of 10 μg / ml, and the positive control was FGF1 (fibroblast growth factor 1). Cells were harvested after 24 hours of culture and treated with Trizol TM Reagent (Thermo Company) was used to extract RNA, and the first-strand cDNA synthesis kit (ReverdAid1) was used to synthesize ReverdAidst StrandcDNASynthesisKit, Thermo Company) to synthesize cDNA. Then, by utilizing a gene amplification instrument (CFX96thermocycler, Bio-Rad company)...
experiment Embodiment 2
[0064] Study on inhibition of collagen degradation by syringin:
[0065] The effect of syringin on improving skin elasticity was evaluated by conducting the following experiment on the effect of inhibiting collagen degradation.
[0066] To maintain skin elasticity, collagen synthesis should be promoted and collagen degradation inhibited. Since collagen is degraded by MMP-1 (matrix metalloproteinase-1), it was checked whether syringin inhibits the expression of collagen-degrading enzymes. Following the same method as in Experimental Example 1, human epithelial cells (NHDF, ATCC) were pretreated for 2 hours with 10 μg / ml syringin.
[0067] Then, they were treated with an inflammatory response-inducing substance (TNFα; 10 ng / ml) that can promote the expression of MMP-1 for 24 hours.
[0068] According to the same method as Experimental Example 1, RNA and cDNA were prepared, and then the expression of MMP-1mRNA was confirmed by Q-PCR ( image 3 ), and by using human MMP-1ELISA ...
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