Preparation method of biological 3D printing ink containing tissue protein compound

Abstract

The invention provides a preparation method of biological 3D printing ink containing a tissue protein compound. The preparation method comprises the following steps: S1, tissue pretreatment: taking tissues, removing non-target components, cleaning with a PBS (Phosphate Buffer Solution), cutting the tissues into small blocks, cracking with a red blood cell lysis buffer, centrifuging, and taking supernatant so as to obtain the pretreated tissues; S2, extraction of the tissue protein compound: grinding the pretreated tissues by using a grinder, taking grinding liquid, carrying out secondary centrifugation, after layering the grinding liquid, taking an aqueous solution layer, carrying out tertiary centrifugation, taking supernate, and filtering by using a 0.22 mu m filter to obtain the tissue protein compound; and S3, preparation of the biological 3D printing ink containing the tissue protein compound: uniformly mixing the biological ink with the tissue protein compound to obtain the biological 3D printing ink. The preparation method has the beneficial effect that the biological 3D printing ink prepared by the invention has relatively high biocompatibility and biological reproducibility, meets the requirements of physiological or pathological mechanism exploration and tissue engineering repair, and has a relatively good clinical application prospect.

Description

technical field [0001] The invention relates to the field of medical model manufacturing and application, in particular to a preparation method of bio-3D printing ink containing tissue protein complex. Background technique [0002] 3D bioprinting technology is an emerging biomanufacturing technology, which simulates the microenvironment of tissues or organs in the body to the greatest extent by using cells and specific bioinks, and then manufactures 3D functional living tissues. Therefore, 3D bioprinting technology can be used to manufacture in vitro tissue / disease models, drug development and screening applications, understand disease development and progression, and fabricate tissues and organs for regenerative medicine, etc. [0003] Bio-ink is a key part of 3D bio-printing technology. In recent years, many natural or synthetic materials with good mechanical properties, biocompatibility and tissue regeneration have emerged, such as: gelatin, sodium alginate, methacryloyl ...

AI Technical Summary

Problems solved by technology

[0004] However, due to the wide variety of biologically active factors in the body, the distribution is different, and the mechanism of action is complex, the microenvironment created by simply adding one or several factors is far from the real in vivo environment, and cannot reproduce the cells and their extracellular matrix in vivo. complexity of

Images