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Umbilical cord mesenchymal stem cell exosome as well as extraction method and application thereof

A technology of mesenchymal stem cells and extraction methods, applied in the field of umbilical cord mesenchymal stem cell exosomes and their extraction, can solve the problems of difficult to obtain long-term bone repair, limited ability of directional differentiation, low cell survival ratio, etc., to improve aging Bone marrow mesenchymal stem cells, enhance osteogenic ability and proliferation ability, and improve the effect of aging state

Pending Publication Date: 2021-12-21
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the treatment of senile osteoporosis-related bone defects (SORBD) by transplanting "healthy" stem cells in bone defects, the survival rate of cells after transplantation is low, the ability of directional differentiation is limited, the characteristics of stem cells are gradually lost, and it is difficult to obtain long-term effects in the end. For bone repair and other problems, the present invention provides a method for extracting umbilical cord mesenchymal stem cell exosomes. The umbilical cord mesenchymal stem cell exosomes extracted by the method of the present invention can be used as active ingredients to prepare and treat senile Drugs for osteoporosis-related bone defects

Method used

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  • Umbilical cord mesenchymal stem cell exosome as well as extraction method and application thereof
  • Umbilical cord mesenchymal stem cell exosome as well as extraction method and application thereof
  • Umbilical cord mesenchymal stem cell exosome as well as extraction method and application thereof

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Effect test

Embodiment 1

[0047] A method for extracting exosomes from umbilical cord mesenchymal stem cells, comprising the steps of:

[0048] 1. Primary culture of umbilical cord mesenchymal stem cells:

[0049] (1) Wash the umbilical cord once in phosphate buffered saline (PBS) containing double antibody, cut the umbilical cord into 2 cm long pieces after washing, and then wash once in phosphate buffered saline containing double antibody, Then the umbilical cord was dissected longitudinally, one umbilical vein and two umbilical arteries were removed, and the umbilical cord Huatong jelly tissue was obtained; cut into 1mm 3 The tissue block (the umbilical cord is derived from the umbilical cord of a normal full-term healthy newborn by cesarean section, and is obtained under the premise of the consent of the mother and her family);

[0050] (2) Place the obtained tissue block in a petri dish, place the petri dish in 5% CO 2 , Place it upside down in an incubator at 37°C for 2 hours, so that the tiss...

Embodiment 2

[0063] A method for extracting exosomes from umbilical cord mesenchymal stem cells, comprising the steps of:

[0064] 1. Primary culture of umbilical cord mesenchymal stem cells:

[0065] (1) Wash the umbilical cord three times in phosphate buffered saline (PBS) containing double antibodies, cut the umbilical cord into 3 cm long pieces, and wash once in phosphate buffered saline containing double antibodies. Then the umbilical cord was dissected longitudinally, and one umbilical vein and two umbilical arteries were removed to obtain the umbilical cord Huatong jelly tissue; the umbilical cord Huatong jelly tissue was washed 4 times in phosphate buffer saline containing double antibodies, and then the cut into 1mm 3 The tissue block (the umbilical cord is derived from the umbilical cord of a normal full-term healthy newborn by cesarean section, and is obtained under the premise of the consent of the mother and her family);

[0066] (2) Place the obtained tissue block in a petr...

Embodiment 3

[0077] A method for extracting exosomes from umbilical cord mesenchymal stem cells, comprising the steps of:

[0078] 1. Primary culture of umbilical cord mesenchymal stem cells:

[0079] (1) Wash the umbilical cord three times in phosphate buffered saline (PBS) containing double antibodies, cut the umbilical cord into 2 cm long pieces, and wash once in phosphate buffered saline containing double antibodies. Then the umbilical cord was dissected longitudinally, and one umbilical vein and two umbilical arteries were removed to obtain the umbilical cord Huatong jelly tissue; the umbilical cord Huatong jelly tissue was washed 5 times in phosphate buffer saline containing double antibodies, and then the cut into 1mm 3 The tissue block (the umbilical cord is derived from the umbilical cord of a normal full-term healthy newborn by cesarean section, and is obtained under the premise of the consent of the mother and her family);

[0080] (2) Place the obtained tissue block in a petr...

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Abstract

The invention discloses an umbilical cord mesenchymal stem cell exosome as well as an extraction method and application thereof. According to the extraction method disclosed by the invention, the umbilical cord mesenchymal stem cell exosome is extracted by using the umbilical cord mesenchymal stem cells pretreated by rapamycin, and the exosome is used for preparing the medicine for treating the bone defect related to the senile osteoporosis; the mechanism of the medicine for treating senile osteoporosis-related bone defect (SORBD) is that senile bone marrow mesenchymal stem cells are improved through the extracted umbilical cord mesenchymal stem cell exosome, and the osteogenic capacity and proliferation capacity of the bone marrow mesenchymal stem cells can be remarkably improved, so that bone defect repair is promoted.

Description

technical field [0001] The invention relates to the technical field of histocytology, in particular to an umbilical cord mesenchymal stem cell exosome and an extraction method and application thereof. Background technique [0002] With the development of biological science, material science, chemistry and other disciplines, especially the development of tissue engineering technology, the repair and replacement of human tissues and organs has entered a new stage. Critical bone defect has always been a thorny problem faced by clinical orthopedic surgeons, and clinical treatment is difficult. The treatment of Senile Osteoporosis Related Bone Defect (SORBD) is even more difficult. [0003] The decrease in the number of bone marrow mesenchymal stem cells (BMSCs) and the dysfunction caused by aging are the decisive factors for the development of senile osteoporosis (Senile Osteoporosis, SO), and it is also the bone marrow associated with senile osteoporosis. The main pathologica...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/51A61K35/28A61P19/10A61P19/08
CPCC12N5/0665A61K35/51A61K35/28A61P19/10A61P19/08C12N2509/00C12N2509/10C12N2501/999
Inventor 胡勇李羡张宁宁通
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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