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Molecules for indicating toxicity producing capacity of aflatoxin producing bacteria and application thereof

An aflatoxin and virulence technology, applied in analytical materials, biological material analysis, material testing products, etc., can solve problems such as difficulty in meeting advance warning and guidance for prevention and control, lack of molecular early warning research examples, and difficulty in meeting, and achieve practical The effect of strong performance, easy operation, and easy promotion and application

Pending Publication Date: 2021-12-24
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is still no example of molecular early warning research before microbial toxin contamination such as aflatoxin at home and abroad, which is difficult to meet the urgent needs of early warning
[0003] Existing aflatoxin early warning methods are mainly established based on aflatoxin detection technology, which are used to evaluate the level of toxin pollution or the degree of postpartum pollution and consumption risk assessment. Once the detection is found, the pollution has often occurred, and it is difficult to meet the requirements of early warning and guidance of prevention and control. Urgent request

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of Molecular AFT-YJFZP008 of Yellowlaminin Catinumi

[0052] In accordance with the following formula, the recipe medium: 3% (w / v) sucrose, 0.3% (w / v) naNO3, 0.1% (w / v) K2HPO4, 0.05% (w / v) MgSO4 · 7H2O, 0.05% ( W / V) KCl, 0.001% (W / V) FESO4, pH 6.5, and configured to obtain a Castes medium. Random selection of public literature "China's typical flower production area of ​​Huang Shujin distribution, China" Henzy-2, Hubha-24, JXZS-29-2, LNCT-6, GXFC-34, GDZJ-122-2, JSNT-1, HUNDX-7, HBHA-8-17, etc., each inoculated to the above In the culture medium, after 5 days of 200 rpm / min, it is conventional method to sufficiently homogenate, crushing the cells, and purified by a routine protein purification system, protein electrophoresis, immune affinity, etc. Macrostatic Molecular AFT-YJFZP008. The test results show that AFT-YJFZP008 can be prepared in the above-mentioned drug strain culture, and HBHA-8-17 is prepared in the same culture conditions, ...

Embodiment 2

[0071] Example 2 Nano-antibody prepared by indicating a molecular AFT-YJFZP008 of a virulence of a yellow belantrimeric

[0072] The AFT-YJFZP008 is used as an immune antigen, and it can be developed using a conventional manner to immunize alpacal or BALB / C rats.

[0073] The prepared AFT-YJFZP008 described above is dissolved in conventional PBS buffer or physiological saline to a concentration of not less than 0.1 mg / ml, and then a complete adjuvant of Frego complete adjuvant, and through the back subcutaneous or intraced multi-point injection method. Immune alpaca, then enhance immunization once every 2-4 weeks, enhance immunization, with Freud's incomplete adjuvant to replace Ford's complete adjuvant. Using conventional ELISA processes to monitor the immune effect, after the oil blood, the oil is no longer rising, then the intravenous blood of the immune alpaca, the total RNA, the synthesis of cDNA, the VHH gene, the recovery of the VHH gene fragment, VHH gene Connect to bi...

Embodiment 3

[0075]Preparation Example 3 indicates aflatoxin producing virulent toxigenic molecules AFT-YJFZP008 embodiment a monoclonal antibody

[0076] The AFT-YJFZP008 is used as an immune antigen, and it can be developed using a conventional manner to immunize alpacal or BALB / C rats.

[0077] The AFT-YJFZP008 prepared above was dissolved in a conventional saline or PBS buffer to a concentration of not less than 0.1mg / mL, then an equal volume of Freund's complete adjuvant and emulsified by mixing, or by subcutaneous injection at multiple sites intradermally manner BALB / c mice, 2-4 weeks after booster immunization every 1, was replaced with Freund's incomplete adjuvant boosted when Freund's complete adjuvant. A conventional ELISA process monitoring immune effect, to BALB / c mice titer does not rise, the subsequent separation of immunized mouse splenocytes, spleen cells with murine myeloma cells SP2 / 0 fusion, semi-solid medium in which hybridoma cells completion of the cultivation m...

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PUM

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Abstract

The invention relates to molecules for indicating the toxicity producing capacity of aflatoxin producing bacteria and application thereof. The amino acid sequence of the molecules AFT-YJFZP008 for indicating the toxicity producing capacity of the aflatoxin producing bacteria is as shown in SEQ ID NO. 1. The molecules are used for identifying the aflatoxin producing capacity of the aspergillus aflatoxin producing strain and identifying whether the aflatoxin producing strain with strong toxicity producing capacity exists in farmlands, agricultural products and feeds or not, and is easy to popularize and apply.

Description

Technical field [0001] The present invention relates to a molecule indicating a virulence of yellowlacin to virulence and its use indicating a molecule of virus viroline. Background technique [0002] Huangquer istrus velocity, harmful, is the most pollutant of pollution food, and in recent years, pollution is presented, and the food safety and people are seriously threatened. Huangquat is the strongest toxic toxin in nature, in which the Huangquisin B1 is the Cancer Cancer, INTERC, has caused too many livestock poisoning incidents. One of the main causes of high-risk cases of liver cancer. According to nearly 5 years, Web of Science retrieved data: Huangquisin pollution food and raw materials have exceeded 110 species, and the first place in high contaminants. However, so far, there is still no molecular warning research before microbial toxin contamination such as yellow guisoxin at home and abroad, and it is difficult to meet the urgent needs of the pre-warning. [0003] The e...

Claims

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Application Information

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IPC IPC(8): C07K14/38G01N33/569G01N33/577G01N33/58G01N33/543
CPCC07K14/38G01N33/56961G01N33/577G01N33/581G01N33/54393G01N2333/38G01N2469/10Y02A50/30
Inventor 张奇唐晓倩白艺珍姜俊李培武
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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