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Extraction method and application of sweet tea polyphenol

A technology of sweet tea polyphenols and extraction method, which is applied in the field of extraction of active ingredients of plants, can solve problems such as limited use of organic reagents, inapplicability to industrial production, and unknown recovery rate, so as to achieve easy collection and acquisition, increase recovery rate, and reduce losses Effect

Active Publication Date: 2022-01-04
HUNAN NUSTREETCARAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many related reports on the extraction and purification of rubusoside and sweet tea polyphenols in sweet tea; in the prior art, the extraction process of sweet tea polyphenols is mostly to wash the effluent of the macroporous resin column with low-concentration ethanol. The preliminary preparation of sweet tea polyphenols is carried out by dehydration and combination. In the subsequent further purification, repeated extraction and gel resin enrichment are used, and organic reagents such as acetone are used. The steps are cumbersome in the scale-up production, and the use of organic reagents is limited. , not suitable for industrial production
[0006] The Chinese invention patent application with the application number CN201510700707.8 discloses a process for the simultaneous preparation of rubusoside and sweet tea total polyphenols. The sweet tea powder is extracted with water, the extract is passed through a macroporous resin column, and the effluent and low-concentration ethanol are collected. The eluent is combined and the two are passed through the macroporous resin column again, and the 40% ethanol eluent is collected again, and the obtained polyphenol purity is greater than or equal to 60%, that is, the polyphenol purity obtained by this process is low, and the yield is lower Low (about 10%)
[0007] The Chinese invention patent application with the application number CN201810688181.X discloses a method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenols. The method specifically includes collecting the effluent from a macroporous resin column and using polyamide The resin is enriched and purified, collected and dried with high concentration ethanol, but the recovery rate is unknown

Method used

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Effect test

Embodiment 1

[0051] The embodiment of the present invention provides a method for extracting sweet tea polyphenols, which specifically includes the following steps:

[0052] S1, take by weighing 20g of powdered sweet tea that has been dried and crushed through a 100 mesh sieve, add water to reflux and extract 3 times, control its solid-liquid ratio to be 1:10, 1:8 and 1:8 respectively, and the extraction temperature is 90°C, After merging, the water extract was obtained, and after being centrifuged at 4000r / min to remove impurities, it passed through a 300-mesh plate and frame filter, and then passed through a D101 macroporous resin column with a diameter-to-height ratio of 1:5 at a flow rate of 2BV / h. After the adsorption is completed, use 2BV of water and 2BV of 70v% ethanol to elute at a flow rate of 1BV / h, collect the eluate from each section, combine the column effluent and water eluate to obtain the sweet tea polyphenol stock solution, 70v% alcohol The eluent is rubusoside stock solu...

Embodiment 2

[0058] The embodiment of the present invention provides a method for extracting sweet tea polyphenols, which specifically includes the following steps:

[0059] S1, take by weighing 5 kg of powdered sweet tea that has been dried and crushed through a 50 mesh sieve, add water and reflux for extraction 3 times, control its solid-liquid ratio to be 1:8, 1:6 and 1:6 respectively, and the extraction temperature is 95° C. After merging, the water extract was obtained, and after being centrifuged at 4000r / min to remove impurities, it passed through a 300-mesh plate and frame filter, and then passed through a D101 macroporous resin column with a diameter-to-height ratio of 1:8 at a flow rate of 3BV / h. After the adsorption is completed, use 3BV of water and 2BV of 70v% ethanol to elute at a flow rate of 2BV / h, collect the eluents of each section, combine the column effluent and water eluents to obtain the original solution of sweet tea polyphenols, 70v% alcohol The eluent is rubusoside...

Embodiment 3

[0065] The embodiment of the present invention provides a method for extracting sweet tea polyphenols, which specifically includes the following steps:

[0066] S1, take by weighing 500kg of powdered sweet tea that has been dried and crushed through a 24-mesh sieve, add water and reflux for extraction 3 times, control its solid-liquid ratio to be 1:8, 1:6 and 1:5 respectively, and the extraction temperature is 100° C. After merging, the water extract was obtained, and after being centrifuged at 4000r / min to remove impurities, it passed through a 300-mesh plate and frame filter, and then passed through a D101 macroporous resin column with a diameter-to-height ratio of 1:10 at a flow rate of 2BV / h. After the adsorption is completed, use 3BV of water and 2BV of 70v% ethanol to elute at a flow rate of 1BV / h, collect the eluents of each section, combine the column effluent and water eluents to obtain the sweet tea polyphenol stock solution, 70v% alcohol The eluent is rubusoside sto...

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Abstract

The invention discloses an extraction method and application of sweet tea polyphenol. The extraction method comprises the following steps of taking a sweet tea polyphenol stock solution obtained after rubusoside is extracted from sweet tea as a raw material, adjusting the pH value, purifying twice by using a macroporous resin column, purifying for the second time by using water eluate collected during the first time of purification, mixing ethanol eluents collected during two times of purification, removing alcohol, diluting, filtering, loading on a polyamide resin column, eluting with water and an ethanol solution in sequence, collecting the ethanol eluent, and carrying out post-treatment. The macroporous resin column is adopted for twice purification, so that the recovery rate can be greatly improved, the operation is simple, and the cost is low; a ethanol solution with the pH of 8-9 is used for elution and desorption, so that the adsorption of the sweet tea polyphenol on polyamide resin can be reduced, and the recovery rate and the product purity are improved; a microfiltration membrane and an ultrafiltration membrane are sequentially used for filtration before passing through the polyamide resin column, so that the subsequent separation and purification are simpler and more convenient; the method can effectively realize full utilization of resources and has a wide application prospect.

Description

technical field [0001] The present invention relates to the technical field of extracting plant effective components, and more specifically, relates to a method for extracting sweet tea polyphenols and its application. Background technique [0002] Guangxi Sweet Tea (Rubus Suavissimus S.Lee) belongs to the Rosaceae Rubus plant, and its main active ingredients include rubusoside, sweet tea polyphenols and sweet tea flavonoids. [0003] Rubusoside is the main component of sweet tea, specifically a terpene component. Because it contains no calories and is 300 times sweeter than sucrose, it is favored by sub-healthy people, especially obese people. Rubusoside is a natural sweetener It is widely used as a food additive, so rubusoside extract has also become a popular product in this industry. [0004] Most sweet tea polyphenols belong to condensed tannins, also known as tannins, and have good water solubility. The content of sweet tea polyphenols in dried sweet tea leaves is as ...

Claims

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Application Information

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IPC IPC(8): A61K36/73C07H15/256C07H1/08
CPCA61K36/73C07H15/256C07H1/08A61K2236/55
Inventor 张宝堂廖娜谭家忠
Owner HUNAN NUSTREETCARAX
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