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Method for extracting and identifying flavonoid metabolites in plum blossom petals

The technology of an extraction method and an identification method is applied in the field of extraction and identification of flavonoid metabolites in plum blossom petals, which can solve the problems of sample waste, high impurities and low purity of the extract, and achieve the effect of less impurities and high purity

Inactive Publication Date: 2022-01-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods for extracting and measuring flavonoids from plum blossom petals have disadvantages such as cumbersome operation steps and a lot of sample waste
[0004] In the prior art, after extraction of flavonoids from plum blossoms, due to the low purity and high impurities of the extracts, accurate identification and analysis of the characteristics of the extracts cannot be carried out, and there is no unified identification standard for the characteristics of flavonoids from plum blossoms

Method used

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  • Method for extracting and identifying flavonoid metabolites in plum blossom petals
  • Method for extracting and identifying flavonoid metabolites in plum blossom petals
  • Method for extracting and identifying flavonoid metabolites in plum blossom petals

Examples

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preparation example Construction

[0030] The preparation method of rutin standard curve in the embodiment of the present invention is:

[0031] (1) Accurately weigh 0.010 g of rutin standard substance, add 60% ethanol extractant to dissolve to 5 ml, and prepare a standard solution with a mass concentration of 2 mg / ml.

[0032] (2) Pipette 25 μl, 50 μl, 100 μl, 150 μl, and 200 μl of the standard solution into 5 test tubes, add 5% NaNO 2 Solution 100μl, shake well and let stand for 6min, add mass fraction 10% Al(NO 3 ) 3 100 μl of the solution, shake well and stand for 6 minutes; add 1000 μl of 4% NaOH solution, add water to 2.5ml, shake well and let stand for 15 minutes.

[0033] (3) the rutin standard solution of different mass concentrations is at 510nm with UV-4802 double-beam scanning type ultraviolet-visible spectrophotometer, with distilled water as blank reference, measure its absorbance, by absorbance value and rutin standard solution concentration Draw a standard curve.

[0034] (4) Find the linear...

Embodiment 1

[0035] Example 1 Extraction and identification analysis of plum blossom petal flavonoids

[0036] This embodiment provides a method for extracting and quantitatively analyzing flavonoids in plum blossom petals, and the specific steps are as follows:

[0037] (1) Take the petals of 'Baixu Zhusha' in full bloom stage as the test material, freeze the fresh plum blossom samples in liquid nitrogen, weigh 0.015-0.025g freeze-dried samples of plum blossom petals with an electronic balance, put them in 2ml of liquid nitrogen quick-frozen samples and grind them In the tube, two steel balls are added to each tube, and the grinding equipment is used for automatic grinding.

[0038] (2) Add the extracting agent with a material-to-liquid ratio of 1:8000 (g / μl), shake it evenly with a vortex instrument, and use an ultrasonic cleaner to sonicate at 25°C; the extracting agent in this example is 60% ethanol, The ultrasonic power of ultrasonic-assisted extraction was 200W, the ultrasonic time ...

Embodiment 2

[0052] Example 2 Extraction and Quantitative Analysis of Flavonoids from Plum Blossom Petals

[0053] The method adopted in this embodiment is the same as that of Example 1, the only difference being that in step (2) of this embodiment, the extraction agent is 2% formic acid methanol, the ultrasonic power of ultrasonic-assisted extraction is 200W, and the ultrasonic time is 30min. Ice cubes were added during this period so that the temperature did not exceed 25°C. The obtained flavonoid extraction rate was 12.9553%. Use Q-TOF-LC-MS liquid chromatography-mass spectrometry instrument to obtain mass spectrometry information, positive mode ion chromatogram see image 3 , negative mode ion chromatogram see Figure 4 . image 3 and Figure 4 The middle peak type is clear and the number of peaks is large, which proves that the flavonoid content in the extract extracted in this embodiment is high and rich in types.

[0054] Metabolites were identified by comparing the m / z values ...

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Abstract

The invention relates to the technical field of extraction and identification of natural compounds, in particular to a method for extracting and identifying flavonoid metabolites in plum blossom petals. The method used by the invention is ultrasonic extraction, the ultrasonic temperature does not exceed 25 DEG C, and the ultrasonic time is 15-45 minutes; and the ultrasonic power is 160 to 200W. The extract obtained by adopting the extraction method disclosed by the invention can be directly used for flavonoid identification, and 31-50 flavonoid compounds can be effectively identified. The extraction and identification method provided by the invention is of great significance to effective evaluation and utilization of plum blossom germplasm resources.

Description

technical field [0001] The invention relates to the technical field of extraction and identification of natural compounds, in particular to a method for extraction and identification of flavonoid metabolites in plum blossom petals. Background technique [0002] Flavonoids are widely found in various plants, and have various physiological activities and medicinal values ​​such as anti-oxidation, anti-tumor, improvement of immune function, and regulation of blood lipids. The human body mainly absorbs flavonoids through eating vegetables and fruits. [0003] Plum blossom is a plant of the genus Prunus in the family Rosaceae. It is native to southern my country and has a cultivation history of more than 3,000 years. Plum blossom is rich in flavonoids. For the extraction process of flavonoids in plum blossoms, there are few relevant studies at present, and there is no formal national standard for the mixture of bioflavonoids in plum blossoms, and there is no systematic and perfe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02G01N30/02
CPCG01N30/02B01D11/0265B01D11/0288
Inventor 张杰吴思惠马召旭高蓉蓉熊涛朱欢欢张俊卫包满珠
Owner HUAZHONG AGRI UNIV