Edible mushroom protein powder with high organic selenium content, and preparation method and application thereof
An edible fungus powder, high organic technology, applied in the field of food processing, can solve problems such as adverse effects of protein biological activity, achieve good adsorption and precipitation effect, good absorption rate, and avoid damage.
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Embodiment 1
[0056] The preparation method of the edible mushroom protein powder of high organic selenium content comprises the following steps:
[0057] S1. Washing and drying the selenium-enriched chrysanthemum to a moisture content of 6%, crushing and passing through an 80-mesh sieve to obtain selenium-enriched chrysanthemum powder;
[0058] S2. Mix selenium-enriched mushroom powder and alkaline water according to the ratio of material to liquid: 1g:15mL, soak for 40min, stir at 200rpm, apply pulse voltage to assist extraction for 40min, filter, centrifuge, and take the supernatant to obtain alkaline extraction liquid;
[0059] S3. Adjust the pH of the alkaline extract step by step to the isoelectric point pH=5, 3.5, 2.5, precipitate and centrifuge at each isoelectric point, collect the precipitates obtained at each isoelectric point respectively, mix them, and obtain the protein crude extract, and the remaining The supernatant is a sugary liquid;
[0060] S4. Adjust the pH of the sug...
Embodiment 2
[0065] The preparation method of the edible mushroom protein powder of high organic selenium content comprises the following steps:
[0066] S1. Washing and drying the selenium-enriched chrysanthemum to a moisture content of 6%, crushing and passing through an 80-mesh sieve to obtain selenium-enriched chrysanthemum powder;
[0067] S2. Mix selenium-enriched mushroom powder and alkaline water according to the ratio of material to liquid: 1g:15mL, soak for 40min, stir at 200rpm, apply pulse voltage to assist extraction for 40min, filter, centrifuge, and take the supernatant to obtain alkaline extraction liquid;
[0068] S3. According to the solid-liquid ratio of 1g:80mL, add a enrichment agent to the alkaline extraction solution, stir at 200rpm at 6°C, and feed carbon dioxide at a speed of 12kg / h at the same time, react until the pH of the solution is 8, filter, respectively Collect the filter cake and filtrate to obtain extract A and extract I;
[0069] S4. Adjust the pH of t...
Embodiment 3
[0077] The preparation method of the edible mushroom protein powder of high organic selenium content comprises the following steps:
[0078] S1. Washing and drying the selenium-enriched chrysanthemum to a moisture content of 6%, crushing and passing through an 80-mesh sieve to obtain selenium-enriched chrysanthemum powder;
[0079] S2. Mix selenium-enriched mushroom powder and alkaline water according to the ratio of material to liquid: 1g:15mL, soak for 40min, stir at 200rpm, apply pulse voltage to assist extraction for 40min, filter, centrifuge, and take the supernatant to obtain alkaline extraction liquid;
[0080] S3. According to the solid-liquid ratio of 1g:80mL, add a enrichment agent to the alkaline extraction solution, stir at 200rpm at 6°C, and feed carbon dioxide at a speed of 12kg / h at the same time, react until the pH of the solution is 8, filter, respectively Collect the filter cake and filtrate to obtain extract A and extract I;
[0081] S4. Adjust the pH of t...
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