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Preparation method and application of nano preparation hydrogel

A hydrogel and micelle technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of poor water solubility of gambogic acid, obvious side effects, and poor curative effect

Pending Publication Date: 2022-01-21
SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gambogic acid has poor water solubility (less than 0.5 μg / mL), high toxicity, obvious side effects of intravenous injection, and poor curative effect, which hinders its clinical transformation

Method used

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  • Preparation method and application of nano preparation hydrogel
  • Preparation method and application of nano preparation hydrogel
  • Preparation method and application of nano preparation hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of the gambogic acid micellar hydrogel provided by the present embodiment is as follows:

[0036] Gambogic acid micelles were mixed with 20 wt% PDLLA-PEG-PDLLA hydrogel at a volume ratio of 1:9 to obtain gambogic acid micellar hydrogels.

[0037]PBS was mixed with 20 wt% PDLLA-PEG-PDLLA hydrogel at a volume ratio of 1:9 to obtain a blank PLEL hydrogel.

[0038] Wherein, the preparation method of gambogic acid micelles used is as follows:

[0039] Preparation of entrapped gambogic acid and methoxypolyethylene glycol-polycaprolactone (mPEG) by film hydration method (solid dispersion method) 2000 - Gambogic acid micelles (GA-MIC micelles) of PCL) polymers. Weigh gambogic acid: mPEG 2000 -The mass ratio of PCL polymer is 5:95, 2.5mg gambogic acid and 47.5mg mPEG 2000 -PCL polymer was placed in a round bottom flask, and gambogic acid and mPEG were dissolved with 2 mL of acetone 2000 -PCL polymer, after completely dissolving, a yellow clear solutio...

Embodiment 2-7

[0044] The preparation method of the gambogic acid micelle hydrogel that embodiment 2-7 provides is basically the same as the preparation method of embodiment 1, and difference is that the volume ratio of gambogic acid micelle and 20wt% PDLLA-PEG-PDLLA is different, See Table 1 below for details.

[0045] Table 1

[0046] Example Volume ratio 2 1:1 3 1:2 4 1:3 5 1:4 6 1:5 7 1:6 8 1:7 9 1:8

experiment example 1

[0048] Investigate the phase transition state of the gambogic acid micellar hydrogel of embodiment 1-9

[0049] The test tube inversion method is used to determine the gelation of gambogic acid micellar hydrogel; the test tube is placed in a constant temperature water bath, and the sample is kept at a constant temperature for 10 minutes at 37 ° C; then it is inverted at 180 degrees to observe whether the sample has Flow phenomenon; if the sample does not flow, it is judged as a gel state, and if it flows, it is judged as a sol state.

[0050] The results show that gambogic acid micelles are mixed with 20wt% PDLLA-PEG-PDLLA in a ratio of 1:3–9 (v / v), and all of them can be gelled, and the gambogic acid of Example 1 and Examples 4-9 The micellar hydrogel is a solution state at room temperature, and is a gel state at 37° C., and the gambogic acid micellar hydrogel in the gel state can recover a solution state at room temperature (see figure 1 with figure 2 ), this process is r...

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Abstract

The invention discloses a preparation method and application of nano preparation hydrogel, and relates to the field of antitumor drugs. The gambogic acid micelle hydrogel disclosed by the invention is prepared by mixing gambogic acid micelles and a hydrogel solution, the gambogic acid micelle hydrogel is in a solution state at room temperature and is in a gel state at body temperature; the gambogic acid micelle hydrogel has good biocompatibility and good biological safety, can slowly release gambogic acid in vivo, is easy for in-situ injection and convenient for clinical application, and has a remarkable anti-tumor effect.

Description

technical field [0001] The present invention relates to the field of antitumor drugs, in particular, to a preparation method and application of a nano-preparation hydrogel, more specifically, to a gambogic acid micelle hydrogel and its preparation method and its application in tumor treatment field applications. Background technique [0002] Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer, accounting for 3.8% of all cancers and 3.6% of cancer deaths. OSCC has a high recurrence rate and is prone to metastasis. The 5-year survival rate of early-stage patients is about 55-60%, and that of advanced-stage patients is about 30-40%. Currently, the standard treatment for OSCC includes surgery, radiotherapy, chemotherapy, and monoclonal antibody therapy. Despite advances in early detection and diagnosis over the past four decades, chemotherapy and radiotherapy often have low response rates, very limited efficacy, and the ability to prolong advanced cancer...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K31/352A61K47/34A61K9/107A61P35/00B82Y5/00B82Y40/00
CPCA61K31/352A61K9/06A61K9/1075A61K47/34A61P35/00B82Y5/00B82Y40/00
Inventor 蔡璐璐童荣生陈新棉杨磊谭素敏
Owner SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
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