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Flexible glucose biosensor and preparation method thereof

A biosensor and glucose technology, applied in instruments, scientific instruments, measuring devices, etc., can solve problems such as poor stability, unsuitable for long-term use, and easy detachment of enzyme membranes, so as to achieve strong stability, avoid enzyme detachment, and enhance sensitivity sexual effect

Pending Publication Date: 2022-01-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme membrane modified by physical methods used in the past flexible biosensors is easy to fall off, has poor stability, and is easily affected by the environment, so it is not suitable for long-term use.

Method used

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  • Flexible glucose biosensor and preparation method thereof
  • Flexible glucose biosensor and preparation method thereof
  • Flexible glucose biosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The flexible glucose biosensor of the present invention includes a flexible substrate, a graphene interdigitated electrode prepared on the flexible substrate, a semiconductor carbon nanomaterial layer, a sensing enzyme film layer, and a blocking encapsulation layer sequentially covered on the graphene interdigitated electrode , and the solid-state electrode wires arranged on both sides of the interdigitated electrodes; wherein the sensing enzyme film layer is fixed on the semiconductor carbon nanomaterial layer through chemical cross-linking.

[0038] Further, an insulating hydrophobic layer is provided around the sensitive interface of the graphene interdigitated electrode. Optionally, the flexible substrate is polyimide (PI) or PDMS. The optional interdigitated electrodes have a single finger width ranging from 200 to 450 μm, and a finger spacing ranging from 50 to 150 μm.

Embodiment 2

[0040] Such as Figure 1-3 Shown, the flexible glucose biosensor prepared by the present invention, its specific process is as follows:

[0041] (1) Interdigital electrodes

[0042] The present invention utilizes laser direct writing technology to prepare interdigitated electrodes, wherein the flexible substrate 1 is PI with a thickness of 80 μm, the wavelength of the laser 2 used in this embodiment is 450nm, the power of the laser probe 3 is 5.5W, and the scanning speed of the laser is 4cm / s. Under this laser parameter, the laser induces the precursor to form a porous graphene electrode. The parameters of the generated interdigital electrodes 4 are that the interdigital spacing is 150 μm, and the single interdigital width is 450 μm.

[0043] In the above steps, the flexible precursor is PET, PC or PVC to prepare interdigital electrodes, which can also be applied to flexible glucose biosensors.

[0044] (2) Solid electrode wire

[0045] as right figure 1 As shown in (a)...

Embodiment 3

[0061] The difference from Example 2 is that in step (1), the flexible substrate 1 is PDMS, and interdigital electrodes are prepared by PDMS transfer printing.

[0062] In the present embodiment, the thickness of the PDMS film 12 is 450 μm, such as Figure 4 As shown, the preparation process includes the following steps:

[0063] (1) Use PI substrate 1 as the precursor of laser-induced technology, with a thickness of 80 μm, and use laser 2 with a wavelength of 450 nm, laser probe 3 with a power of 4 W, and a laser scanning speed of 1 cm / s to induce porous graphene electrode. The parameters of the generated interdigital electrodes 4 are that the interdigital spacing is 50 μm, and the single interdigital width is 200 μm. Place the prepared interdigital electrode 4 facing up in the Petri dish.

[0064] (2) Coating PDMS liquid on the interdigital electrodes 4, and then curing at high temperature to form a PDMS film 12 with a thickness of 450 μm, the curing temperature is 75° C....

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Abstract

The invention discloses a flexible glucose biosensor and a preparation method thereof. The sensor comprises a flexible substrate, a graphene interdigital electrode prepared on the flexible substrate through a laser engraving method, solid electrode wires arranged on the two sides of the interdigital electrode, a semiconductor carbon nanomaterial layer covering the surface of the interdigital electrode, a sensing enzyme membrane layer fixed on the semiconductor carbon nanomaterial layer through chemical crosslinking, and a blocking packaging layer covering the sensing enzyme membrane layer. The chemical cross-linking immobilization comprises the steps of carrying out surface carboxylation treatment on the semiconductor carbon nanomaterial layer, activating surface carboxyl, and immersing the semiconductor carbon nanomaterial layer into a solution containing glucose oxidase for chemical cross-linking immobilization. The flexible biosensor provided by the invention is high in specificity, high in sensitivity, large in detection range, low in detection limit and simple to prepare, has the flexible wearable characteristic and has a wide application prospect.

Description

technical field [0001] The invention relates to a sensor and a preparation method thereof, in particular to a flexible glucose biosensor and a preparation method thereof. Background technique [0002] With the improvement of people's living standards in our country, the incidence of diabetes in the population is gradually increasing. According to the data, there are about 3,000 new diabetic patients in my country every day, and about 1.2 million new patients every year. Diabetes has become an established It is the third chronic disease that seriously threatens human health after tumors and cardiovascular diseases. With such a large population of patients, real-time monitoring of blood sugar levels in diabetic patients is very popular. Most of the previous methods of measuring blood sugar concentration were carried out by drawing blood from the patient. This method can not be used for tracking and will cause physical harm to the patient. According to research, the glucose co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3272
Inventor 赵国孙红迅汪小旵张利涛谢裕名
Owner NANJING AGRICULTURAL UNIVERSITY
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