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Sugarcane pollen preservation method

A preservation method and pollen technology, applied in the direction of plant preservation, botanical equipment and methods, applications, etc., can solve the problems of reducing pollen survival rate and seed setting rate, difficulty in preserving sugarcane pollen, and decreased pollen vigor, so as to improve the survival rate and seed setting rate, eliminate adverse effects, and reduce the effect of pollen discomfort

Pending Publication Date: 2022-01-28
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The cell wall of sugarcane pollen is relatively thin, and it is more obvious to the external climate change. The sugarcane pollen will lose its vitality after one hour of loose pollen, and the preservation of sugarcane pollen is also more difficult.
At present, sugarcane pollen is mostly preserved by low-temperature preservation, but direct low-temperature preservation has problems such as low pollen survival rate and decreased pollen viability; and when sugarcane pollen is released from dormancy, it will cause pollen discomfort, thereby reducing pollen survival rate and seed setting rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preserving sugarcane pollen, comprising the following steps:

[0035] (1) Transplanting: Select the sugarcane plants that have basically completed earing and are about to bloom, cut them down along the middle and lower part of the sugarcane stem, bring them back to the room, insert the sugarcane stem into the nutrient solution with a depth of about 35 cm and place it obliquely. Placed at an angle of 30 degrees to obtain sugarcane plants to be harvested; the nutrient solution consists of 0.01 parts of 0.01% brassinolide water-soluble powder, 40 parts of magnesium sulfate, 15 parts of ammonium sulfate, and 8 parts of sodium nitrate by weight. , 12 parts of superphosphate, 12 parts of superphosphate, and 100 parts of sterile water;

[0036] (2) Collection: Observe the cracking of the anthers of the sugarcane plants to be harvested, and set a 100-watt incandescent lamp at a position 0.5 meters above the top of the flower spike; when the anthers crack, turn on t...

Embodiment 2

[0046] A method for preserving sugarcane pollen, comprising the following steps:

[0047] (1) Transplanting: Select the sugarcane plants that have basically completed earing and are about to bloom, cut them down along the middle and lower part of the sugarcane stem, bring them back to the room, insert the sugarcane stem into the nutrient solution with a depth of about 35 cm and place it obliquely. Placed at an angle of 60 degrees to obtain sugarcane plants to be harvested; the nutrient solution consists of 0.02 parts of 0.01% brassinolide water-soluble powder, 45 parts of magnesium sulfate, 20 parts of ammonium sulfate, and 10 parts of sodium nitrate by weight. , 15 parts of superphosphate, 15 parts of superphosphate, and 130 parts of sterile water;

[0048] (2) Collection: Observe the cracking of the anthers of the sugarcane plants to be harvested, and set a 100-watt incandescent lamp at a position 0.7 meters above the top of the flower spike; when the anthers crack, turn on ...

Embodiment 3

[0058] A method for preserving sugarcane pollen, comprising the following steps:

[0059] (1) Transplanting: Select the sugarcane plants that have basically completed earing and are about to bloom, cut them down along the middle and lower part of the sugarcane stem, bring them back to the room, insert the sugarcane stem into the nutrient solution with a depth of about 35 cm and place it obliquely. Placed at an angle of 45 degrees to obtain sugarcane plants to be harvested; the nutrient solution consists of 0.015 parts of 0.01% brassinolide water-soluble powder, 42 parts of magnesium sulfate, 17 parts of ammonium sulfate, and 9 parts of sodium nitrate by weight. , 13 parts of superphosphate, 14 parts of superphosphate, and 120 parts of sterile water;

[0060] (2) Collection: Observe the cracking of the anthers of the sugarcane plants to be harvested, and set a 100-watt incandescent lamp at a position 0.6 meters above the top of the flower spike; when the anthers crack, turn on ...

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PUM

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Abstract

The invention discloses a sugarcane pollen preservation method. The method comprises the following steps: (1) transplanting; (2) collecting; (3) drying; (4) pretreatment; (5) freezing; and (6) refrigerating. After sugarcane pollen is collected, the moisture content of the sugarcane pollen is immediately reduced to 10% or below, and the survival rate of the sugarcane pollen is increased; the sugarcane pollen is sequentially refrigerated at the temperature of 8 to 10 DEG C, frozen at the temperature of -25 to -30 DEG C and finally stored at the ultralow temperature, the tolerance of the sugarcane pollen can be improved, the frostbite rate can be reduced, meanwhile, performance change caused by too large temperature difference can be avoided, the respiration of the sugarcane pollen can be greatly weakened, the hereditary performance of the sugarcane pollen can be kept stable while the storage time is prolonged, and the storage effect is good; and when the sugarcane pollen is put into a freezer at the temperature of -65 to -70 DEG C for cryopreservation, a drying agent is added into a cryopreservation tube, white paper and oiled paper are wrapped outside the pollen, and when the sugarcane pollen is released from dormancy or power is cut off temporarily in the cryopreservation process, the discomfort of the pollen can be reduced, so that the survival rate and the maturing rate of the sugarcane pollen are improved.

Description

technical field [0001] The invention relates to the technical field of pollen preservation, in particular to a sugarcane pollen preservation method. Background technique [0002] Sugarcane, Saccharum genus, perennial tall solid herb. The rhizome is thick and well developed. Stems 3-5(-6) meters high. China Taiwan, Fujian, Guangdong, Hainan, Guangxi, Sichuan, Yunnan and other southern tropical regions are widely planted. Sugarcane is suitable for planting in places with fertile soil, sufficient sunlight, and large temperature differences between winter and summer. Sugar cane is a temperate and tropical crop that is used as a raw material for the manufacture of sugar and for ethanol as an energy substitute. Sugarcane is rich in sugar and water, and also contains various vitamins, fats, proteins, organic acids, calcium, iron and other substances that are very beneficial to human metabolism. It is mainly used for sugar production. The skin is generally purple and green. , a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 张保青黄玉新段维兴周珊周忠凤杨翠芳张革民高轶静宋修鹏李杨瑞李冬梅
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI