Embedding adapters, anchor molecules, molecular membranes, devices, methods and applications

Abstract

The application discloses an embedding linker, an anchor molecule, a molecular membrane, a device, a method and an application. Among them, the embedded joint is used to be embedded in the amphiphilic molecular membrane, and the embedded joint includes a first hydrophilic segment, a hydrophobic segment and a second hydrophilic segment connected in sequence, the hydrophobic segment is used to span the hydrophobic region of the amphiphilic molecular membrane, and the hydrophobic The segment is rigid; the first hydrophilic segment and / or the second hydrophilic segment are used to attach to the analyte. The embedded linker provided by this application can be enriched in the amphiphilic molecular membrane, increasing the effective detection time of the analyte.

Description

technical field

[0001] The present application belongs to the field of gene sequencing technology, and in particular relates to a device for embedding adapters, anchor molecules, molecular membranes, and nanopores to characterize biomolecules, a method for assembling embedded adapters in amphiphilic molecular membranes, and enriching biomolecules into transmembrane nanopores. Methods for analytes, methods and applications for nanopore characterization of analytes. Background technique

[0002] In existing nanopore sequencing, in a cavity filled with electrolyte, a molecular membrane with nanoscale pores divides the cavity into two small chambers. When a voltage is applied to the electrolyte, ions or other small molecular substances can pass through the small chambers. The pores form a stable and detectable ionic current, and different types of biomolecules can be detected according to the current information.

[0003] Anchor molecules are provided in existing molecular memb...

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