High-performance adipose-derived mesenchymal stem cell solution as well as preparation method and application thereof
A high-performance stem cell technology, which is applied in the field of high-performance adipose-derived mesenchymal stem cell solution and its preparation, can solve problems such as the limitation of sodium hyaluronate, the expansion of adverse indications, and the impact of cell efficacy, so as to shorten the recovery time , Reduce cell damage, strong uniformity and stability
Pending Publication Date: 2022-02-08
成都拜美森生物科技有限公司
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Problems solved by technology
[0004] At present, the commonly used diluted solute for intravenous infusion of cell resuscitation is compound electrolyte solution. The viability and proliferation ability of the resuscitated cells after dilution with this solute will decrease, and the efficacy of the cells will be affected after injection into the joint cavity, so it is generally not used in the joint cavity. Injection; in the prior art, sodium hyaluronate is commonly used as the dilute solute for cell resuscitation in intra-articular injection. Due to the limited use of sodium hyaluronate, it can only be used for local injection, which is not conducive to the expansion of indications under the drug management system
Method used
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Embodiment 1
[0027] Take 1 tube from the liquid nitrogen tank with a cell density of 1.0 x 10 7 / mL cell cryopreservation tube, put it into a 42°C water bath (the temperature of the water bath is set at 42°C, and keep the lid open), gently shake the tube body clockwise, so that the cap is always above the liquid level ; After the cells in the cryopreservation tube are thawed, carry out alcohol disinfection on the cryopreservation tube, then transfer to the ultra-clean bench, open the cover of the cryopreservation tube, and gently blow the cells in the cryopreservation tube 3 times to obtain a cell suspension. The cell suspension was mixed with 10% glucose solution (ie dilute solute) in equal volume to prepare a cell solution.
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Abstract
The invention discloses a high-performance adipose-derived mesenchymal stem cell solution as well as a preparation method and application thereof. A cell suspension is prepared by using an elution-free cell preparation recovery method, and the glucose with the mass fraction of 10% is combined to dilute the cell suspension so as to prepare the high-performance cell solution, so that the cell activity, the apoptosis level and the like in the prepared cell solution are greatly improved, then the cell solution is applied to an arthritis treatment drug, the repair degree of articular cartilage and the curative effect of arthritis are improved, and the cell solution can also be used for whole-body intravenous infusion, gives consideration to indications of whole-body application of the cell preparation, and provides a solution for improving the curative effect of clinical-grade mesenchymal stem cell products.
Description
technical field [0001] The invention relates to the field of medical and biological technology, in particular to a high-performance adipose-derived mesenchymal stem cell solution and its preparation method and application. Background technique [0002] Mesenchymal stem cells (MSCs) have multi-lineage differentiation potential and have been widely used in tissue engineering, cell replacement therapy, regenerative medicine, gene therapy and other fields. In the prior art, the long-term storage of mesenchymal stem cells is mainly in the form of cryopreservation, and the clinically used MSCs need to be injected or infused into the human body after resuscitation, so it is necessary to dilute the cell suspension with clinical-grade solutes. [0003] In the prior art, the recovery methods of mesenchymal stem cells mainly include two methods, (1) conventional recovery method: incubate the complete medium at 37°C, take out the cell cryopreservation tube from the liquid nitrogen tank,...
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Login to View More IPC IPC(8): C12N5/0775A61K35/28A61K9/08A61P19/02
CPCC12N5/0667A61K35/28A61K9/08A61K9/0019A61P19/02
Inventor 朱依谆
Owner 成都拜美森生物科技有限公司