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Multi-functional photo-thermal nano bactericidal material and preparation and application thereof

A nano-sterilization and photothermal technology, which is applied in the field of antibacterial materials, can solve the problems of inability to kill bacteria and side effects at the same time, and achieve the effects of fast adsorption rate, shortened adsorption time, and large adsorption capacity

Active Publication Date: 2022-02-18
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention solves the disadvantages that the preparations for adsorbing endotoxin in the prior art have toxic and side effects, and cannot kill bacteria at the same time

Method used

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  • Multi-functional photo-thermal nano bactericidal material and preparation and application thereof
  • Multi-functional photo-thermal nano bactericidal material and preparation and application thereof
  • Multi-functional photo-thermal nano bactericidal material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Preparation of a multifunctional nano-photothermal sterilizing material M-PDA that can specifically adsorb endotoxin

[0051] figure 1 Preparation of M-PDA nanoparticles flow chart for the present invention. The preparation steps are as follows:

[0052] Step 1: Add endotoxin (ET), surfactant and ammonia water to cyclohexane, stir evenly with magnetic force, and disperse by ultrasonic for 10 minutes;

[0053] Step 2: Add dopamine solution, continue to ultrasonically disperse for 10 minutes to form a microemulsion; magnetically stir the above microemulsion at room temperature for 24 hours, break the emulsion, and collect the precipitate by centrifugation.

[0054] Step 3: wash the precipitate with absolute ethanol and 3% acetic acid aqueous solution respectively, and finally wash 3 times with endotoxin-free depyrogenated pure water to obtain polydopamine nanoparticles (M- PDA)

[0055] As a control, the preparation method of non-imprinted polydopamine nano...

Embodiment 2

[0056] Embodiment 2: Optimization of reaction conditions

[0057] First, the dosage of endotoxin was fixed, the concentration of dopamine DA was adjusted, a series of M-PDA and N-PDA were prepared, and the adsorption effect of each M-PDA and N-PDA on 250 ng / mL endotoxin was compared to determine the optimal DA concentration. Then, maintain the optimal concentration of DA, adjust the amount of endotoxin, prepare a series of M-PDA and N-PDA, and evaluate their adsorption effect on endotoxin.

[0058] Depend on figure 2 A in A shows that when the amount of DA added is between 2.5-10 mg, as the amount of DA increases, the amount of endotoxin adsorbed by M-PDA and the imprinting factor also increase. When the amount of DA was 10 mg, the adsorption amount of endotoxin and the imprinting factor of M-PDA reached the maximum value, and the adsorption amount and the corresponding imprinting factor showed a downward trend when the amount of DA continued to increase. The reason may be t...

Embodiment 3

[0059] Embodiment 3: M-PDA and N-PDA characterization

[0060] Depend on image 3 A in and image 3 In B, it can be seen that the M-PDA and N-PDA nanoparticle morphology prepared in Example 1 are all spherical structures, and the particle size is relatively uniform, which is about 75nm, indicating that DA is in the inverse microemulsion system. Polymerization occurred, and polydopamine nanoparticles were successfully prepared.

[0061] Depend on Figure 4 Visible, FT-IR spectral analysis shows that the M-PDA prepared in embodiment 1 and the N-PDA nanoparticle are all in 3410cm -1 、2929cm -1 、1605cm -1 、1510cm -1 and 1295cm -1 There are obvious characteristic absorption peaks at , corresponding to phenolic hydroxyl, -CH 2 -, benzene ring, amino group and phenolic C-O and other groups. It indicated that DA was successfully polymerized in microemulsion droplets, and polydopamine nanoparticles were generated.

[0062] Table 1 shows the results of dynamic light scattering...

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Abstract

The invention relates to a multi-functional photo-thermal nano bactericidal material as well as preparation and application thereof, and belongs to the field of antibacterial materials. The invention aims to prepare a multi-functional photo-thermal sterilization material capable of specifically adsorbing endotoxin. Polydopamine nanoparticles M-PDA capable of selectively adsorbing endotoxin are prepared by taking dopamine as a functional monomer and endotoxin as a template molecule and adopting a reversed-phase microemulsion method, so that bacteria can be efficiently killed by utilizing photothermal conversion, the endotoxin released by G-bacteria can be effectively adsorbed, and secondary damage caused by the endotoxin in photothermal sterilization is reduced. According to the invention, an inverse emulsion polymerization method is utilized for the first time to prepare a multi-functional photo-thermal sterilization material, and polydopamine nanoparticles capable of rapidly killing bacteria under the irradiation of near-infrared light and selectively adsorbing endotoxin are prepared.

Description

technical field [0001] The invention belongs to the field of antibacterial materials, and more specifically relates to a multifunctional photothermal nano-bacteriac material and its preparation and application, especially to a multifunctional nano-photothermal material capable of selectively adsorbing endotoxins by using an inverse emulsion polymerization method. A method for the sterilization material M-PDA, and its application in photothermal sterilization. Background technique [0002] With the widespread use of antibiotics, the resistance of bacteria to antibiotics is increasing rapidly, and some multi-drug resistant bacteria (Multi-drug resistant, MDR) even show a certain degree of resistance to all current antibiotics, so that they are infected by these bacteria Afterwards, there is no drug available in the treatment (Vet Microbiol, 2014, 171(3-4): 273-278), in order to effectively control bacterial infection in order to deal with the infection of drug-resistant bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/14A61P31/04B82Y5/00B82Y40/00
CPCA61K41/0052A61K9/14A61P31/04B82Y5/00B82Y40/00
Inventor 吕斌谭多戚俊峰
Owner HUAZHONG UNIV OF SCI & TECH
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