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SDF-1alpha/CGRP-loaded HAp-SF artificial periosteum and preparation method thereof

A technology of SDF-1- and SDF-1, which is applied in the field of biomedical materials, can solve the problems of artificial periosteum, etc., and achieve good effects of promoting angiogenesis, migration, and cell adhesion

Pending Publication Date: 2022-03-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no mature "artificial periosteum" product in clinical application. The artificial periosteum in the existing research includes: ① degradable polymer porous membrane loaded with BMP, VEGF and other bioactive factors prepared by electrospray technology (Wang Shen Guo, Wang Honghe, Liu Hao, Ren Hongwen. Bioabsorbable artificial periosteum and its preparation method. 2008-02-27.); ② Mineralized collagen artificial periosteum prepared by pressing, freeze-drying and other processes using nano-calcium phosphate and collagen fibers (Tao Chunsheng, Qiu Zhiye, Song Tianxi, Zhang Ziqiang, Cui Fuzhai. A mineralized collagen artificial periosteum and its preparation method. 2014-10-15.), etc., have not yet seen the research and development of artificial periosteum with the function of slow-release SDF-1α and CGRP. Related Products

Method used

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  • SDF-1alpha/CGRP-loaded HAp-SF artificial periosteum and preparation method thereof
  • SDF-1alpha/CGRP-loaded HAp-SF artificial periosteum and preparation method thereof
  • SDF-1alpha/CGRP-loaded HAp-SF artificial periosteum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Preparation of CGRP-HAp-SF artificial periosteum

[0065] Step 1: remove 100 g of chrysalis cocoons and immerse in 0.02mol / L Na 2 CO 3 solution, heated to 95°C, stirred continuously for 30 minutes, fished out the silk fibroin, washed 3-5 times with deionized water, and dried in an oven at 60°C to obtain silk fibroin;

[0066] Step 2: Configure CaCl with a molar ratio of 1:2:8 2 , CH 3 CH 2 OH, H 2 O ternary solvent

[0067] Step 3: Dissolve the dried silk fibroin after degumming in step 1 in step 2, heat to 70°C, and keep stirring until the silk fibroin is completely dissolved;

[0068] Step 4: filter the silk fibroin solution dissolved in step 3 with degreasing gauze to remove insoluble impurities to obtain a silk protein solution;

[0069] Step 5: Put the silk fibroin solution obtained in step 4 into a dialysis bag with a molecular weight of 3500, place the dialysis bag in deionized water, and perform low-temperature dialysis in a refrigerator at 4°C,...

Embodiment 2

[0083] Step 1: remove 110 g of chrysalis cocoons and immerse in 0.03mol / L Na 2 CO 3 solution, heated to 95°C, stirred continuously for 45 minutes, fished out silk fibroin, rinsed with deionized water for 3-5 times, and dried in an oven at 70°C to obtain silk fibroin;

[0084] Step 2: Configure CaCl2, CH3CH2OH, H2O ternary solvent with a molar ratio of 1:2:8

[0085]Step 3: Dissolve the dried silk fibroin after degumming in step 1 in step 2, heat to 75°C, and keep stirring until the silk fibroin is completely dissolved;

[0086] Step 4: filter the silk fibroin solution dissolved in step 3 with degreasing gauze to remove insoluble impurities to obtain a silk protein solution;

[0087] Step 5: Put the silk fibroin solution obtained in step 4 into a dialysis bag with a molecular weight of 3500, place the dialysis bag in deionized water, and perform low-temperature dialysis in a refrigerator at 4°C, and replace the dialysate every 4-6 hours until the solvent is completely removed...

Embodiment 3

[0101] Step 1: remove 120 g of chrysalis cocoons and immerse in 0.04mol / L Na 2 CO 3 solution, heated to 95°C, stirred continuously for 60 minutes, fished out silk fibroin, washed 3-5 times with deionized water, and dried in an oven at 80°C to obtain silk fibroin;

[0102] Step 2: Configure CaCl2, CH3CH2OH, H2O ternary solvent with a molar ratio of 1:2:8

[0103] Step 3: Dissolve the dried silk fibroin after degumming in step 1 in step 2, heat to 80°C, and keep stirring until the silk fibroin is completely dissolved;

[0104] Step 4: filter the silk fibroin solution dissolved in step 3 with degreasing gauze to remove insoluble impurities to obtain a silk protein solution;

[0105] Step 5: Put the silk fibroin solution obtained in step 4 into a dialysis bag with a molecular weight of 3500, place the dialysis bag in deionized water, and perform low-temperature dialysis in a refrigerator at 4°C, and replace the dialysate every 4-6 hours until the solvent is completely removed I...

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Abstract

The invention discloses an SDF-1 alpha / CGRP-loaded HAp-SF artificial periosteum and a preparation method thereof, and belongs to the field of orthopedic implant materials, the SDF-1 alpha and CGRP factors are added to degradable macromolecules as main raw materials, and the nHA / SF absorbable artificial periosteum loaded with SDF-1 alpha and CGPR factors is prepared by an electrostatic spinning method. The prepared artificial periosteum has the performance of loading and slowly releasing SDF-1-alpha and CGPR factors, so that the artificial periosteum has good osteogenesis and angiogenesis activity and biocompatibility. Besides, the artificial periosteum can play a barrier role in the local defect part, can effectively prevent surrounding soft tissue from growing in and heterotopic ossification from occurring, further has better mechanical strength and biodegradability, and can be used as a repairing material for bone tissue defect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to HAp-SF artificial periosteum loaded with SDF-1α / CGRP and a preparation method thereof. Background technique [0002] Bone defect is a common clinical disease in orthopedics, which can be caused by various factors such as trauma, infection, and tumor. Large bone defects are one of the challenges in clinical orthopedics treatment. Currently, bone grafting is the most commonly used clinical treatment method, that is, to induce bone regeneration by implanting scaffolds to fill bone defects. However, after the bone scaffold is implanted, the defect area undergoes a passive connection and filling process, and lacks the active recognition of the microenvironment of the bone defect by the material. Although some scaffolds are integrated with exogenous active factors, which can promote angiogenesis and bone regeneration, it is still difficult for the natural bone remodeling ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/46A61L27/48A61L27/54A61L27/58A61F2/28D04H1/728
CPCA61F2/28A61F2/30942A61F2/30965A61L27/54A61L27/227A61L27/58A61L27/46A61L27/48D04H1/728A61F2002/2817A61F2002/2835A61F2002/3006A61F2002/30062A61F2002/30064A61F2002/30772A61L2400/12C08L89/00
Inventor 杜昶姚孟宇吴晶晶
Owner SOUTH CHINA UNIV OF TECH
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