Construction method and application of iris plant eleutherine eleutherine VIGS silencing system

Abstract

The invention discloses a construction method and application of a VIGS (Vinyl Insulin Growth Sulfate) silencing system of an iridaceae plant eleutherine eleutherine. The VIGS silence system suitable for the eleutherine eleutherine is successfully created by taking the TRV as a carrier of a VIGS system and taking an eleutherine eleutherine phytoene dehydrogenase gene as a target gene and utilizing a vacuum infiltration treatment method; the system is applied to development of gene function research of Euphorbia elegans pigment synthesis related genes EbMYB5 and EbMYB52, the expression quantity of the EbMYB5 and EbMYB52 genes in infected silence treatment group sample leaves is remarkably reduced, and the expression quantity of key genes related to pigment synthesis routes is remarkably changed to different degrees; the eleutherine americana VIGS silencing system can be successfully applied to eleutherine americana gene function verification. The eleutherine americana VIGS silence system constructed by the invention has important significance on eleutherine americana gene function research.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a method for constructing a VIGS silencing system of the Iridaceae plant shallot and its application. Background technique [0002] Eleutherine bulbosa (Mill.) Urb., a plant of the Iridaceae family, is native to the West Indies, the Americas, etc., and was later introduced and cultivated by folks in Yunnan, and developed into a traditional folk medicinal plant in Southwest China such as Yunnan and Guangxi. Red onion has multiple functions such as antibacterial and anti-inflammatory (Padhi and Panda, 2015), and can also be eaten as wild vegetables (Pan Jun et al., 2011; Wang Xiaoyi et al., 2015; Xu Qiaolin et al., 2014). Iridaceae red onion is similar to the well-known vegetable Allium ascalonicum L. (Allium ascalonicum L.) in its bulb appearance and name, so it is easy to be confused. In the 1980s, studies on the chemical constituents and pharmacolo...

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Problems solved by technology

[0003] Although research on the chemical constituents and pharmacological activities of red onion has been carried out (Jiang et al., 2018; Xu Xiaomei et al., 2018), there are very few studies on its molecular biology, especially the lack of gene sequence information and its own genetic transformation. The system has not yet been constructed, resulting in a relatively lagging research on its gene expression analysis, functional research and synthesis mechanism of effective active ingredients

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