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Rare-earth/nucleotide supermolecular assembly body-based mercury ion detection method

A supramolecular and mercury ion technology, applied in the field of analytical chemistry, can solve the problems of unreported and low cost, and achieve the effect of good selectivity, low cost and rapid detection

Active Publication Date: 2020-02-21
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of supramolecular fluorescent materials with lower cost single rare earth ions and nucleotides for the detection of heavy metal mercury ions has not been reported yet.

Method used

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  • Rare-earth/nucleotide supermolecular assembly body-based mercury ion detection method
  • Rare-earth/nucleotide supermolecular assembly body-based mercury ion detection method
  • Rare-earth/nucleotide supermolecular assembly body-based mercury ion detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In this example, the rare earth terbium ion / guanine mononucleotide supramolecular material was prepared and characterized.

[0041] (1) Combine guanine mononucleotide and Tb(NO 3 ) 3 Reaction stock solutions (10 mM) were prepared by dissolving in HEPES buffer (0.1M, pH 7.4) and PBS buffer (0.1M, pH 7.4). at Tb(NO 3 ) 3 When dissolved in PBS buffer, a light white turbid solution was obtained, presumably Tb 3+ The reaction was carried out with phosphate, so HEPES buffer was selected for subsequent reactions.

[0042] (2) With stirring, add 1 mL of TbCl to 1 mL of guanine mononucleotide stock solution 3 solution (10mM), react at room temperature for 30min. The precipitate was collected by centrifugation (10000 rpm, 5 min). The precipitate was redispersed in deionized water and centrifuged (10000rpm, 5min). This process was repeated twice, and the resulting white precipitate was dried under vacuum to obtain a purified supramolecular complex of rare earth terbium ion...

Embodiment 2

[0049] In this example, Hg 2+ The selectivity of the detection was determined.

[0050] The supramolecular complex of rare earth terbium ion / guanine mononucleotide obtained in step (2) of Example 1 was dispersed in HEPES buffer (0.1M, pH 7.4, 2mL) to prepare rare earth terbium ion / guanine mononucleotide Nucleotide supramolecular complexes with a concentration of 10mg / mL solution, take 14 parallel samples (100μL), take 1 group as the control group, add Hg to the remaining groups respectively 2 + , Ag + ,Al 3+ , Ca 2+ ,Cd 2+ ,Co 2+ ,Cu 2+ ,Fe 2+ ,Mg 2+ ,Mn 2+ , Ni 2+ ,Pb 2+ ,Zn 2+ . Keep the metal ion concentration at 1 μM, the supramolecular complex concentration at 5 mg / mL, and the total solution volume at 200 μL by adding HEPES buffer. After adding the metal ions at an interval of 10 min, the change of the fluorescence intensity (Ex: 285nm) was measured by a JASCO FP6500 spectrophotometer. Take the ratio of fluorescence intensity (fluorescence intensity after ...

Embodiment 3

[0052] In this example, Hg 2+ The detection response time is analyzed.

[0053] The supramolecular complex of rare earth terbium ion / guanine mononucleotide obtained in step (2) of Example 1 was dispersed in HEPES buffer (0.1M, pH 7.4, 2mL) to prepare rare earth terbium ion / guanine mononucleotide A solution of supramolecular complexes of nucleotides with a concentration of 10 mg / mL; take 100 μL of the solution and add Hg 2+ and HEPES buffer to ensure that the final supramolecular complex concentration is 5mg / mL, Hg 2+ The total volume of 1 μM solution is 200 μL, and the fluorescence intensity changes with time are measured by JASCO FP6500 spectrophotometer; 2+ The supramolecular complex solution of rare earth terbium ion / guanine mononucleotide was used as blank group (NPs). The ratio of fluorescence intensity (fluorescence intensity / fluorescence intensity of blank group) was plotted against time. See the results Figure 5 , the fluorescence of supramolecular complexes of r...

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Abstract

The invention provides a rare-earth / nucleotide supermolecular assembly body-based mercury ion detection method, and belongs to the field of chemical analysis. The detection method is rapid, sensitiveand accurate, detection can be completed within 10 minutes, the detection method is rapid in response to mercury ion and good in selectivity, the detection limit can reach 2.6nM, a favorable linear relation is achieved between 0 micrometer and 0.6 micrometer, and Hg<2+> with naked-eye visual detection being 0.2 micrometer can be achieved; a supermolecular complex is stable in fluorescent signal and does not produce light-bleach and can be obtained by a one-step solution reaction method, the preparation process is simple and easy to operate and is low in cost and good in repeatability; and by the detection method, application of rear-earth terbium ion / guanine nucleotide supermolecular complex in mercury ion detection is achieved, rapid detection of site Hg<2+> can be achieved by combining site detection fluorescent small-size equipment so as to handle an abrupt condition, data is provided for an abrupt pollution condition, and the method has favorable application prospect.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for detecting mercury ions based on a rare earth / nucleotide supramolecular assembly, in particular to a method for detecting mercury ions based on a supramolecular complex of rare earth terbium ion / guanine mononucleotide method. Background technique [0002] With the rapid development of industry and the sharp increase of population, human beings are facing the great challenge of environmental pollution. Heavy metal mercury ions pose a major threat to human health due to their high toxicity, bioaccumulation, and difficulty in degradation. [0003] The country currently strictly stipulates that the mercury content in surface water and drinking water shall not exceed 1ppb and 50ppt respectively. Traditional mercury detection methods include inductively coupled plasma mass spectrometry (ICP-MS), atomic emission spectrometry (AES), atomic absorption spectrometry (AAS) and...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 周丽马戈梁振达闫兵
Owner GUANGZHOU UNIVERSITY
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