Immune cell activator as well as preparation method and application thereof
A technology of immune cells and activators, which is applied in the direction of cell culture active agents, blood/immune system cells, biochemical equipment and methods, etc. It can solve the problems of poor ability to induce differentiation, weak tumoricidal activity, and low amplification factor, etc. question
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Embodiment 1
[0044] The preparation of the immune cell activator and its application to the cultivation of NK cells comprise the following steps:
[0045] (1) The formula of the immune cell activator is as follows: Mycobacterium phlei F.U.36 injection 50 μg / ml, levamisole 50 μg / ml, ribonucleic acid 200 μg / ml, cefaclor 100 μg / ml, cefodizime 100 μg / ml, ceftazidime 100 μg / ml, CD50 monoclonal antibody 10μg / ml, CD52 monoclonal antibody 10μg / ml, vitamin A 1mg / ml, vitamin C 5mg / ml, vitamin E 1mg / ml, heparin sodium 500IU / ml, recombinant human albumin 10μg / ml, recombinant human immune Globulin 10 μg / ml, recombinant human transferrin 50 μg / ml. After the preparation was completed, it was added to the ALyS505N-AC medium at a ratio of 1:100.
[0046] (2) Use heparin sodium anticoagulant blood collection tubes to collect 40ml of peripheral blood from healthy people, slowly pour 20ml of fresh blood on 15ml of lymphatic separation fluid, centrifuge at 800g for 20min, and slowly rise and fall. First col...
Embodiment 2
[0073] (1) The activator is applied to the cultivation of CIK cells, including the following steps:
[0074] The concentration of the main components of the cellular immune activator is Mycobacterium phlei F.U.36 injection 50 μg / ml, levamisole 50 μg / ml, ribonucleic acid 200 μg / ml, cefaclor 100 μg / ml, cefodizime 100 μg / ml, ceftazidime 100 μg / ml ml, CD50 monoclonal antibody 10 μg / ml, CD52 monoclonal antibody 10 μg / ml, vitamin A 1 mg / ml, vitamin C 5 mg / ml, vitamin E 1 mg / ml, heparin sodium 500 IU / ml, recombinant human albumin 10 μg / ml, recombinant human immunoglobulin Protein 10μg / ml, recombinant human transferrin 50μg / ml. After the preparation was completed, it was added to the ALyS505N-0 medium at a ratio of 1:100.
[0075] (2) Collect 40ml of peripheral blood from healthy people using heparin sodium anticoagulant blood collection tubes, slowly pour 20ml of fresh blood on 15ml of lymphatic separation fluid, centrifuge at 800xg for 20min, slowly rise and fall. First collect th...
Embodiment 3
[0093] The activator is applied to the culture of γδT cells, including the following steps:
[0094] (1) Immune cell activators are as follows: Mycobacterium phlei F.U.36 injection 50 μg / ml, levamisole 50 μg / ml, ribonucleic acid 200 μg / ml, cefaclor 100 μg / ml, cefodizime 100 μg / ml, ceftazidime 100 μg / ml ml, CD50 monoclonal antibody 10 μg / ml, CD52 monoclonal antibody 10 μg / ml, vitamin A 1 mg / ml, vitamin C 5 mg / ml, vitamin E 1 mg / ml, heparin sodium 500 IU / ml, recombinant human albumin 10 μg / ml, recombinant human immunoglobulin Protein 10μg / ml, recombinant human transferrin 50μg / ml. After the configuration is completed, add it to the ALyS505N-0 medium at a ratio of 1:100.
[0095] (2) Collect 40ml of peripheral blood from healthy people using heparin sodium anticoagulant blood collection tubes, slowly pour 20ml of fresh blood on 15ml of lymphatic chaotropic fluid, centrifuge at 800xg for 20min, slowly rise and fall. First collect the upper layer of plasma with a sterile pipette ...
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