Sample density separation medium and cell separation method

A density separation and sample technology, applied in the field of cytology preparation, can solve the problems of affecting the observation of samples, inability to separate, and affecting the preparation effect, and achieve the effect of clear observation, simple operation, and clear and clean detection background.

Pending Publication Date: 2022-03-08
山东高创医疗器械国家研究院有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The main component of the existing sample density separation liquid is a synthetic sucrose polymer, referred to as polysucrose, but after adding the density separation liquid to the preservation liquid, the unresolved particles at the layered part of the preservation liquid and the sample density separation liquid The red blood cells that are completely lysed in the preservation solution will be in contact with polysucrose to condense into a string of money and deposit at the bottom of the tube and mix with the sinking cells of diagnostic significance (epithelial cells, tumor cells), which will affect the observation of the sample after subsequent film production
In addition, the traditional sample density separation fluid does not have the function of removing mucus, and because the mucus wraps the cells, it cannot be separated by density centrifugation, which also affects the final production effect

Method used

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  • Sample density separation medium and cell separation method
  • Sample density separation medium and cell separation method
  • Sample density separation medium and cell separation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Prepare separation liquid according to the following ratio:

[0040] Table 1 Experimental group sample separation solution formula

[0041]

[0042] Table 2 Control group sample separation solution formula

[0043]

[0044]

[0045] The preparation method of sample density separation liquid comprises:

[0046] ①Add weighed sodium chloride, potassium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, and disodium edetate into all purified water, and stir for 20 minutes to obtain solution A.

[0047] ②Add weighed dithiothreitol, tea saponin and sodium sulfite to liquid A and stir for 5 minutes to obtain solution B.

[0048] ③Add weighed phenoxyethanol to liquid B, mix and stir for 5 minutes to obtain solution C.

[0049] ④ Add sodium hydroxide to make the pH between 6.8-7.2.

[0050] ⑤ The preparation environment is carried out under normal temperature and pressure.

[0051] Effect verification

[0052] According to the formula recorded ...

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Abstract

The invention relates to the technical field of cytological flaking, in particular to a sample density separation medium and a cell separation method. The sample density separation liquid provided by the invention is prepared from a solvent, disodium ethylene diamine tetraacetate, disodium hydrogen phosphate, monopotassium phosphate, potassium chloride, sodium chloride, dithiothreitol, tea saponin and sodium sulfite. The mucus in the sample can be effectively softened and decomposed, the detection background is clear and clean, and the cell morphology is clearly observed.

Description

technical field [0001] The invention relates to the technical field of cytology, in particular to a sample density separation liquid and a cell separation method. Background technique [0002] Cervical cancer is currently the only cancer with a clear cause that can be detected and prevented early. Therefore, early screening of cervical cancer is of great significance to the prevention of cervical cancer. Liquid-based cytometry, TCT for short, is a new cytology film production technology introduced in recent years, and it is a major innovation in the field of film production. Compared with traditional smears, liquid-based cytology preserves specimens through preservation solution, and then undergoes a series of pre-processing techniques to remove impurities in the specimens and make a thin layer that can be observed clearly under the microscope, making it easier for readers to observe. Its diagnostic accuracy and sensitivity are significantly improved compared with tradition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/09
CPCC12N5/0633C12N5/0693C12N2509/00
Inventor 张明伟刘美静孙欣欣李婧盛春海蓝皓慧
Owner 山东高创医疗器械国家研究院有限公司
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