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Preparation method of nano-enzyme and glycosaminoglycan composite nanofiber dressing

A technology of composite nanofibers and glycosaminoglycans, applied in the field of medical materials, can solve the problems of full contact with unfavorable inflammatory microenvironment, strict melting point requirements, and inappropriate nanoenzyme materials, and achieve the effect of improving the anti-inflammatory effect.

Active Publication Date: 2022-03-15
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]At present, the methods of stably loading nanozymes on the fiber surface mainly include high-temperature in-situ growth, blend spinning, etc., but the former has strict requirements on the melting point of the material and is not suitable for the preparation of Nanozyme material whose temperature is higher than the melting point of the fiber; the composite fiber prepared by the latter embeds the nanozyme inside the fiber, which is not conducive to its full contact with the inflammatory microenvironment and exerts the function of eliminating ROS

Method used

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  • Preparation method of nano-enzyme and glycosaminoglycan composite nanofiber dressing
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  • Preparation method of nano-enzyme and glycosaminoglycan composite nanofiber dressing

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Experimental program
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Effect test

Embodiment 1

[0033] 1) Prepare polycaprolactone with a mass ratio of 15%, dissolve it in a trifluoroethanol solution with stirring at 60° C. for 4 hours, and prepare a nanofiber membrane by electrospinning. Electrospinning parameters are: spinning speed 0.42mm / s, needle 22G, distance 15cm, voltage 15kv, temperature 28°C, humidity 48%.

[0034] 2) Prepare Prussian blue particles with a concentration of 4 mg / mL and disperse them in absolute ethanol solution by ultrasonic, and stably load the Prussian blue particles on the surface of the obtained nanofiber membrane by electrostatic spraying. Electrostatic spraying parameters are: electrospray rate 0.0015mm / s, needle 25G, distance 6.5cm, voltage 21kv. The temperature is 32°C and the humidity is 40%.

[0035] 3) Under nitrogen protection, heparin sodium and carboxyl activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS ) (the molar ratio of EDC and NHS is 2: 1) was reacted in the acidic dimo...

Embodiment 2

[0037]1) Prepare polycaprolactone with a mass ratio of 12%, dissolve it in a trifluoroethanol solution with stirring at 40° C. for 6 h, and prepare a nanofiber membrane by electrospinning. Electrospinning parameters are: spinning speed 0.38mm / s, needle 22G, distance 16cm, voltage 12.5kv, temperature 30°C, humidity 38%.

[0038] 2) Prussian blue particles with a concentration of 6 mg / mL were prepared and dispersed in a dimethyl sulfoxide solution by ultrasonic waves, and the Prussian blue particles were stably loaded on the surface of the obtained nanofibrous membrane by electrostatic spraying. Electrostatic spraying parameters are: EFI speed 0.0010mm / s, needle 27G, distance 5cm, voltage 22kv. The temperature is 30°C and the humidity is 45%.

[0039] 3) Heparin sodium and carboxyl activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) (the molar ratio of EDC and NHS 2: 1) was reacted in acidic dimorpholine ethanesulfonic acid buff...

Embodiment 3

[0041] 1) Prepare polycaprolactone with a mass ratio of 14%, dissolve it in a trifluoroethanol solution with stirring at 50° C. for 5 h, and prepare a nanofiber membrane by electrospinning. Electrospinning parameters are: spinning speed 0.40mm / s, needle 21G, distance 18cm, voltage 13.8kv, temperature 32°C, humidity 40%.

[0042] 2) Prepare Prussian blue particles with a concentration of 5 mg / mL and disperse them in a mixed solution of absolute ethanol and dimethyl sulfoxide with a volume ratio of 1:1 by ultrasonic, and stably load the Prussian blue particles on the obtained nanofibrous membrane by electrostatic spraying surface. Electrostatic spraying parameters are: EFI speed 0.0006mm / s, needle 27G, distance 7cm, voltage 20kv. The temperature is 28°C and the humidity is 42%.

[0043] 3) Heparin sodium and carboxyl activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) (the molar ratio of EDC and NHS 2: 1) was reacted in acidic ...

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Abstract

The invention relates to the field of medical materials, and discloses a nano enzyme and glycosaminoglycan composite nanofiber dressing preparation method, which comprises: (1) dissolving a polymer in a solvent A, and carrying out electrostatic spinning to prepare a nanofiber membrane; (2) dispersing nano-enzyme in a solvent B, and loading the nano-enzyme on the surface of the nanofiber membrane through electrostatic spraying; (3) modifying the glycosaminoglycan by using dopamine hydrochloride to obtain a dopamine-containing glycosaminoglycan active matter; and (4) depositing the active matter in the composite nanofiber membrane through oscillation treatment to obtain the composite nanofiber dressing. According to the preparation method, electrostatic spinning and electrostatic spraying technologies are combined, nano-enzyme is uniformly sprayed and loaded on the surface of a dressing substrate to obtain a nanofiber membrane, then active matter is loaded on the nanofiber membrane, and the composite nanofiber dressing is obtained. Nano enzyme and glycosaminoglycan are loaded on the surface of the material at the same time, and the anti-inflammatory effect can be stably and synergistically achieved in a long-acting mode.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a preparation method of a nanozyme and glycosaminoglycan composite nanofiber dressing. Background technique [0002] At present, the aging of the global population is becoming more and more prominent, which has brought about a series of thorny problems of chronic wounds such as ulcers and bedsores. At present, the main problem of chronic wounds is that the healing process is slow and often stays in the inflammatory stage. Due to the complexity of the microenvironment inside and outside cells, the mechanism of persistent inflammatory wounds is still unclear. At present, drugs with anti-inflammatory effects mainly enter the body through direct oral or injection methods, but these methods prevent the drugs from targeting the inflammatory site, and the circulation in the body is likely to cause side effects on the body. Stable loading of materials with anti-inflammatory function on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/38A61L15/44A61L15/28A61L15/26A61L15/42D04H1/728B05D1/04
CPCA61L15/38A61L15/44A61L15/28A61L15/26A61L15/42D04H1/728B05D1/04A61L2400/12A61L2300/236A61L2300/254A61L2300/41A61L2300/45C08L67/04C08L79/04C08L5/10C08L5/08Y02A50/30
Inventor 吴金丹李萌萌杨扬毛峥伟
Owner ZHEJIANG SCI-TECH UNIV