Nucleic acid amplification product quantitative detection instrument and nucleic acid amplification product quantitative detection method

A technology for amplification products and quantitative detection, applied in biochemical equipment and methods, microbial determination/inspection, biochemical instruments, etc. , to reduce the formation of aerosols, reduce the diffusion of amplification products, and facilitate the operation of opening and sealing

Pending Publication Date: 2022-03-15
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The first object of the present invention is to provide a nucleic acid amplification product quantitative detection instrument, which directly uses the nucleic acid amplification reaction container (PCR amplification tube) as a cuvette, and performs the quantitative detection of the nucleic acid amplification product in the PCR amplification tube Detection, to solve the quantitative detection results that cannot be obtained by visually observing the color development results of nucleic acid dyes, the spectrophotometer and ultra-micro cuvettes lead to time-consuming and laborious detection processes, the long-term exposure of the sample solution to the environment leads to contamination of the experimental environment and instruments, and false positive test results defects, making the quantitative detection of nucleic acid amplification products more convenient, and the detection results more reliable

Method used

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  • Nucleic acid amplification product quantitative detection instrument and nucleic acid amplification product quantitative detection method
  • Nucleic acid amplification product quantitative detection instrument and nucleic acid amplification product quantitative detection method
  • Nucleic acid amplification product quantitative detection instrument and nucleic acid amplification product quantitative detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The housing structure of the optical detection module is as follows: figure 2 As shown, the aperture of the sample hole 2 is constant, the shape of the sample hole 2 is cylindrical, the air guide hole 4 is located on the side wall of the sample hole 2, the apertures of the light holes 3 on both sides of the sample hole 2 are 3 mm, and the light source is a red color with a diameter of 3 mm. The light-emitting diode and the photoelectric sensor are photosensitive diodes with a diameter of 3mm.

[0054] The signal acquisition module uses ADS1110 analog-to-digital conversion module, the main control module uses STC5A60S2 single-chip microcomputer, the display module uses LCD1602 liquid crystal display, and the input module uses tact switches.

[0055] The power module uses a 18650 lithium battery and a 5V charging and discharging integrated module from Shenzhen Xintaiwei Technology Co., Ltd.

[0056] The usage method of this embodiment is as follows:

[0057] Nucleic ac...

Embodiment 2

[0074] The housing structure of the optical detection module is as follows: image 3 As shown, the shape of the sample hole 2 is cylindrical, the air guide hole 4 is located at the bottom of the sample hole 2, the diameters of the light holes 3 on both sides of the sample hole 2 are 3mm and 5mm respectively, the light source is a red light-emitting diode with a diameter of 3mm, and the photoelectric sensor is a diameter of 5mm photodiodes.

[0075] The signal acquisition module uses ADS1110 analog-to-digital conversion module, the main control module uses STC5A60S2 single-chip microcomputer, and uses a 2.4-inch touch-sensitive TFT screen as the input and output modules.

[0076] The power module uses the LP803450x5V battery produced by Zhongshun Xinneng Battery Co., Ltd., which integrates the boost protection board and lithium battery.

[0077] The preparation and detection steps of the liquid to be tested are the same as in Example 1.

Embodiment 3

[0079] The housing structure of the optical detection module is as follows: Figure 4 As shown, the shape of the sample hole 2 is cylindrical on the upper part and V-shaped on the lower part. The air guide hole 4 is located at the bottom of the sample hole 2. The apertures of the light holes 3 on both sides of the sample hole 2 are 5 mm in diameter, and the light source is a red light-emitting diode with a diameter of 5 mm. The photoelectric sensor is a photoresistor with a diameter of 5mm.

[0080] The preparation and detection steps of the liquid to be tested are the same as in Example 1.

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Abstract

The invention provides a nucleic acid amplification product quantitative detection instrument and a nucleic acid amplification product quantitative detection method.The nucleic acid amplification product quantitative detection instrument comprises an optical detection module and a detection control module and is characterized in that the detection instrument comprises an optical detection module shell, and the optical detection module shell is provided with a sample hole for containing a PCR amplification tube; light through holes vertically intersected with the sample hole are formed in the two sides of the sample hole in the optical detection module shell, and a light source and a photoelectric sensor are arranged in the light through holes in the two side faces of the optical detection module shell respectively; a nucleic acid amplification reaction container (PCR amplification tube) is directly used as a cuvette for quantitative detection of nucleic acid amplification products, so that the defects that quantitative detection results cannot be obtained when nucleic acid dye developing results are observed by naked eyes, and a spectrophotometer needs to transfer sample liquid, so that the detection process is time-consuming and labor-consuming, and pollution and false positive results are easily caused are overcome; the rapid quantitative detection of a nucleic acid amplification product is realized.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid amplification product quantitative detection instrument and a nucleic acid amplification product quantitative detection method. Background technique [0002] Nucleic acid amplification technology is widely used in the fields of medical diagnosis, animal and plant quarantine, food safety, and genetically modified detection. It has the advantages of high sensitivity and specificity. The amplified product realizes the detection of the target substance. Therefore, the simple and rapid quantitative determination of nucleic acid amplification products is of great significance for the development of rapid detection methods based on nucleic acid amplification technology. [0003] The quantitative analysis methods of nucleic acid amplification products mainly include real-time fluorescence quantitative method and gel imaging analysis. The fluorescence real-...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/6851G01N21/78G01N21/31
CPCC12Q1/6851G01N21/78G01N21/31C12Q2531/113C12Q2565/607
Inventor 唐文志颜廷婷李忠宏
Owner NORTHWEST A & F UNIV
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