Mixed catalyst and application thereof in protein content determination
A technology of mixing catalysts and catalytic assistants, which is applied in the detection field, can solve the problems of long sample digestion time, environmental pollution, and detection efficiency reduction, and achieve the effects of short sample digestion processing time, improved catalytic efficiency, and reduced detection costs
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Embodiment 1
[0030] The preparation of embodiment one catalyst 1
[0031] Preparation of catalyst promoter
[0032] Pretreatment: Measure methacryloxysilane and add it dropwise to 1wt% acetic acid aqueous solution, while stirring until the solution is clear and transparent, to obtain methacryloxysilane with a mass fraction of methacryloxysilane of 2wt%. Hydrolyzate, spare; take carbon airgel powder and add it to a ball mill, spray and add methacryloxysilane hydrolyzate, add agate balls, carbon airgel powder and solid-liquid of methacryloxysilane hydrolyzate The ratio is 1:5, the mass ratio of carbon airgel powder and agate ball is 1:20, and the ball milling is carried out for 16h under the condition of 150r / min at a rotating speed. Dry.
[0033] Loading: Take 1.0g ferrous ammonium sulfate and dissolve it in 10ml hydrazine hydrate to obtain ferrous ammonium sulfate solution, add 0.25g pretreated carbon airgel powder to 15ml ethylene glycol, after ultrasonic dispersion, add ferrous sulfate...
Embodiment 2
[0036] The preparation of embodiment two catalyst 2
[0037] Preparation of catalyst promoter
[0038] Pretreatment: Measure methacryloxysilane and add it dropwise to 1wt% acetic acid aqueous solution, while stirring until the solution is clear and transparent, to obtain methacryloxysilane with a mass fraction of 1wt% methacryloxysilane Hydrolyzate, spare; take carbon airgel powder and add it to a ball mill, spray and add methacryloxysilane hydrolyzate, add agate balls, carbon airgel powder and solid-liquid of methacryloxysilane hydrolyzate Ratio is 1:20, the mass ratio of carbon airgel powder and agate ball is 1:50, under the condition that rotating speed is 180r / min, carry out ball milling 15h, after ball milling finishes, filter and obtain carbon airgel powder, dry after washing Dry.
[0039] Loading: Take 1.2g ferrous ammonium sulfate and dissolve it in 10ml hydrazine hydrate to obtain ferrous ammonium sulfate solution, add 0.2g pretreated carbon airgel powder to 15ml et...
Embodiment 3
[0042] The preparation of embodiment three catalysts 3
[0043] Preparation of catalyst promoter
[0044] Pretreatment: Measure methacryloyloxysilane and add it dropwise to 1wt% acetic acid aqueous solution, while stirring until the solution is clear and transparent, to obtain methacryloyloxysilane with a mass fraction of methacryloyloxysilane of 1.5wt%. Silane hydrolyzate, standby; take carbon airgel powder and add in ball mill, spray and add methacryloxysilane hydrolyzate, add agate balls, carbon airgel powder and methacryloxysilane hydrolyzate The liquid ratio is 1:12, the mass ratio of carbon airgel powder and agate ball is 1:35, and the ball milling is carried out for 15h under the condition of 200r / min at a rotating speed, and the carbon airgel powder is obtained by filtering after the ball milling is completed. After washing drying.
[0045] Loading: Take 2.0g ferrous ammonium sulfate and dissolve it in 15ml of hydrazine hydrate to obtain ferrous ammonium sulfate solu...
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