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Comprehensive method for preventing and treating aging of tissue culture seedlings and application

A comprehensive method and technology for tissue culture seedlings are applied in the comprehensive method and application field for preventing and treating tissue culture seedling aging, which can solve the problems of plant aging, excessive accumulation of toxic substances, lack of nutrients in medium, and achieve easy operation and cost reduction. Effect

Pending Publication Date: 2022-04-01
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In tissue culture, too long subculture time will cause lack of nutrients in the medium, excessive accumulation of toxic substances, etc. will lead to premature senescence of plants

Method used

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  • Comprehensive method for preventing and treating aging of tissue culture seedlings and application
  • Comprehensive method for preventing and treating aging of tissue culture seedlings and application
  • Comprehensive method for preventing and treating aging of tissue culture seedlings and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 adopts anti-oxidant to control the aging of cauliflower tissue culture seedling

[0029] The test material is cauliflower tissue culture seedlings which have been subcultured for 3 years. The growth vigor of the tissue culture seedlings is obviously weakened, and the growing points appear to die. Tissue culture seedlings with similar growth vigor and consistent plant size were selected for the test.

[0030]VC (ascorbic acid), DTT (dithiothreitol), PVP (polyvinylpyrrolidone) and AgNO 3 (Silver nitrate) 4 kinds of antioxidants were respectively configured into 100mg / ml, 50mg / ml, 50mg / ml and 5mg / ml aqueous solutions, which were sterilized by suction filtration sterilization in the ultra-clean bench, and stored in centrifuge tubes for later use. The test uses MS medium, add 30g / L sucrose and 7g / L agar powder, adjust the pH to 5.8 before sterilization, after high temperature sterilization, when the medium is cooled to 60-70℃, add 4 kinds of antioxidants respec...

Embodiment 2

[0034] Example 2 Adjusting the osmotic pressure of the medium to prevent the aging of cauliflower tissue culture seedlings

[0035] The test material is the same as that used in Example 1. Tissue culture seedlings with similar growth vigor and consistent plant size were selected for the test.

[0036] In the experiment, MS was used as the basic medium, and the medium was prepared by using 1, 1 / 2, 1 / 4 multiples of MS inorganic salt concentration and 10g / L, 20g / L sucrose concentration respectively. MS+10 g / L sucrose, MS+20 g / L sucrose, 1 / 2MS+10 g / L sucrose, 1 / 2MS+20 g / L sucrose, 1 / 4MS+10 g / L sucrose and 1 / 4MS+10 g / L sucrose equal proportion medium. 7g / L agar powder was uniformly used as the medium, and the pH was adjusted to 5.8 before sterilization. After high-temperature sterilization, the medium was divided into 100ml wide-mouth Erlenmeyer flasks, and 30ml of medium was added to each bottle. Afterwards, the aged cauliflower tissue culture seedlings were inoculated into th...

Embodiment 3

[0040] Example 3 Adding Exogenous Phytohormones to Prevent Aging of Cauliflower Tissue Cultured Seedlings

[0041] The test material is the same as that used in Example 1. Tissue culture seedlings with similar growth vigor and consistent plant size were selected for the test.

[0042] IAA (indole acetic acid), GA 3 (Gibberellin) was pre-dissolved with a small amount of absolute ethanol, then added sterile water to prepare 1 mg / ml mother liquor, sterilized by suction filtration sterilization in the ultra-clean bench, and added to the centrifuge tube , and then stored in the dark at 4°C for later use. The test uses MS medium, add 30g / L sucrose and 7g / L agar powder, adjust the pH to 5.8 before sterilization, after high temperature sterilization, when the medium is cooled to 60-70°C, add plant exogenous hormones Medium, then divide the medium into 100ml wide-mouth Erlenmeyer flasks, and add 30ml of medium to each bottle. Hormone combinations added to the medium are 0.5 mg / L IA...

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Abstract

The invention discloses a comprehensive method for preventing and treating aging of tissue culture seedlings. The comprehensive method mainly comprises the following three methods: (1) adopting an antioxidant; (2) regulating the osmotic pressure of the culture medium; and (3) adding exogenous plant hormones. The method disclosed by the invention can effectively relieve aging of the tissue culture seedlings and improve rejuvenation of the tissue culture seedlings, and experimental results show that according to the three methods, the osmotic pressure of the culture medium is changed by respectively adding an antioxidant and plant exogenous hormone with certain concentration and adjusting the concentration of inorganic salt and cane sugar or the three methods are mixed for use; the broccoli aging plant can be promoted to continuously grow, the aging of the tissue culture seedling can be effectively relieved, and the broccoli aging seedling can be rejuvenated.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a comprehensive method and application for preventing and treating aging of tissue culture seedlings. Background technique [0002] Plant tissue culture technology is an important means of plant research and production. In the process of plant tissue culture, long-term subculture of tissue culture seedlings will cause aging phenomena such as weakened growth potential, growth stagnation, or growth point necrosis, which seriously affects the normal progress of plant tissue culture research and production, thereby causing a greater impact on the tissue culture industry. influences. [0003] The aging of tissue culture seedlings is mostly caused by physiological aging caused by long-term asexual reproduction. The main reasons for the aging of tissue culture seedlings are: firstly, genetic reasons. Plants have formed a certain aging mechanism during the evol...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘玉冬刘艳军许丁帆黄俊轩李建科武春霞梁兴
Owner TIANJIN AGRICULTURE COLLEGE
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