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Cultivation method for artificially inducing ricefield eel to directly and directionally develop into male ricefield eel

A cultivation method and directional development technology, which is applied in the field of artificially inducing the direct directional development of eels into males, can solve the problems of inaccessibility and low inductive conversion efficiency, and achieve the effect of important application value and high survival rate.

Active Publication Date: 2022-04-08
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methyltestosterone treatment in rice field eel embryos and juvenile eels can induce early sex reversal in some rice field eels, but the induction conversion efficiency is very low, and they cannot obtain functional sperm (Zhao Yunfang, Ke Xuntao, Effects of exogenous hormones inducing sex transformation in rice field eels

Method used

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  • Cultivation method for artificially inducing ricefield eel to directly and directionally develop into male ricefield eel
  • Cultivation method for artificially inducing ricefield eel to directly and directionally develop into male ricefield eel
  • Cultivation method for artificially inducing ricefield eel to directly and directionally develop into male ricefield eel

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Experimental program
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Effect test

Embodiment 1

[0023] A method for artificially inducing rice field eels to directly develop into males without going through the female maturity stage, comprising the following steps:

[0024] 1. Artificial breeding

[0025] Parent selection: the parent rice field eel used in the present invention was purchased from the Baishazhou aquatic product market in Wuhan. Select the pregnant female eel with bulging abdomen, plump and granular feeling, and the male eel with larger body, yellowish body color and concave cloaca. The shallow water in the indoor water tank is placed in a quiet environment for a period of time and then used for artificial induced breeding.

[0026] Artificial oxytocin: The weight of a female eel is about 100 grams, and the oxytocin dose injected into each female eel is: 3 mg of carp pituitary gland, 300 units of chorionic gonadotropin HCG, and 0.8 micrograms of luteinizing hormone-releasing hormone A2. Most female eels matured eggs 36 hours after hormone injection. Mal...

Embodiment 2

[0036] The fecundity test of rice field eels that are directly induced to develop into males:

[0037] Utilize the method described in embodiment 1, the swamp eel that letrozole induces to directly develop into male grows to 1 year of sexual maturity after stopping the treatment and feeding common feed for 6 months, which is the breeding period of wild swamp eel (Hubei The breeding season of field eels in the area is from May to July every year). The fecundity detection and analysis of directional induction and direct development into male rice field eels were carried out during the breeding period of rice field eels. The gonads of rice field eels were induced to be male by anatomical orientation, and the morphological structure of the gonads was observed by histological section. The fertilization of eggs of wild female eels by collecting the mature sperms induced to be male eels can obtain normally developed eel embryos and juveniles.

[0038] (1) Anatomical characteristics...

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Abstract

The invention belongs to the technical field of genetic breeding of aquatic animals, and discloses a cultivation method for artificially inducing finless eels to directly develop into male finless eels, which comprises the following steps: A, domesticating juvenile finless eels to eat a compound feed; b, feeding a compound feed containing letrozole; c, when the ricefield eels fed with the letrozole grow to 6 months old, gonads are converted into testis, and the ricefield eels directly develop into male. The method is easy to implement and simple and convenient to operate, and 1-age ricefield eels are directly induced into male ricefield eels with normal reproductive capacity. According to the method, the finless eels which are directly developed into the male without being subjected to the female maturation stage can be directly obtained, and the method can be effectively applied to finless eel fry production and genetic breeding research and has important application value.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal genetics and breeding, and more specifically relates to a cultivation method for artificially inducing ricefield eels to directly develop into males. By adopting the method, ricefield eels of one age can be directly cultivated into males with reproductive ability. The method is suitable for obtaining male rice field eel broodstock for seed production in a short period of time and on a large scale in rice field eel breeding, and quickly obtaining new rice field eel germplasm with improved traits in the process of rice field eel genetic breeding. Background technique [0002] Fish sex differentiation is affected by genetic factors and environmental factors. Sex hormones in dioecious fish such as carp and mandarin fish play an important role in sex differentiation. Estrogen has the function of maintaining female sexual characteristics, and androgen has the function of maintaining male sexual c...

Claims

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Application Information

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IPC IPC(8): A01K61/10A23K50/80A23K20/132
CPCY02A40/81
Inventor 宋焱龙蒋银军陶彬彬陈戟罗红瑞朱作言胡炜
Owner INST OF AQUATIC LIFE ACAD SINICA