Method and system for centrifugally separating polyhydroxyalkanoate by using horizontal screw
A technology of polyhydroxyalkanoate and decanter centrifugation, which is applied in the field of extraction and separation of polyhydroxyalkanoate, can solve the problems of difficult large-scale industrial application, frequent cleaning, low efficiency, etc., so that it is not easy to block equipment and reduce energy consumption , the effect of improving the separation efficiency
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[0020] According to a preferred embodiment of the present invention, the method for described decanter centrifugal separation comprises:
[0021] (2-1) performing the first decanter centrifugal separation on the slurry to obtain the first thick phase rich in polyhydroxyalkanoate and the first supernatant;
[0022] (2-2) The first dense phase rich in polyhydroxyalkanoate is subjected to a second decanter centrifugal separation to obtain a second thick phase rich in polyhydroxyalkanoate and a second supernatant.
[0023] According to the present invention, preferably, the temperature of the first decanter centrifugal separation is 20-70° C., preferably 20-50° C., and the differential speed is 2-60 rpm.
[0024] According to the present invention, preferably, the temperature of the second decanter centrifugal separation is 20-70°C, preferably 20-50°C, and the differential speed is 2-60rmp.
[0025] According to the present invention, preferably, step (2-2) also includes mixing t...
preparation example 1
[0073] This preparation example is used to illustrate the preparation of polyhydroxyalkanoate fermentation broth
[0074] Halomonas was inoculated in the seed medium for primary activation culture at 37°C and 200rpm, and cultivated to OD 600 Reach about 4, obtain first-grade seed liquid;
[0075] Inoculate the primary seed liquid into the seed medium with an inoculation amount of 10% by volume, carry out secondary activation culture at 37°C and 200rpm, and cultivate to OD 600 Reach about 4, obtain secondary seed liquid, obtain fermented seed liquid.
[0076] Then, the secondary seed liquid with an inoculum amount of 10 volume % is inserted into the initial fermentation medium, and the fermentation system is directly fermented without sterilization. Control the temperature at 37°C, control the speed at 600-1000rpm, control the ventilation rate at 0.5-2.0vvm, control the initial dissolved oxygen above 30%; control the sugar concentration between 5-20g / L by feeding materials du...
Embodiment 1
[0078] This embodiment is used to illustrate the separation method of the polyhydroxyalkanoate provided by the invention
[0079] (1) Transfer the polyhydroxyalkanoate fermentation broth prepared in Preparation Example 1 to a continuous slagging disc centrifuge and perform centrifugation at 37°C and a rotation speed of 9000rpm to obtain the first bacteria containing polyhydroxyalkanoate Somatic cells and first fermentation supernatant.
[0080] (2) Transfer the first fermentation supernatant to an intermittent slagging disc centrifuge for centrifugation at 37°C and 10,000rpm to obtain the second bacterial cells containing polyhydroxyalkanoate and the second fermentation supernatant Serum.
[0081] (3) Mix the first bacterial cell and the second bacterial cell with water, the volume of the water is 3 times the volume of the first bacterial cell and the second bacterial cell, after stirring evenly, transfer to the continuous dish Centrifuge separation under the conditions of 3...
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