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Method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants

An antioxidant, fat-soluble technology, applied in the field of separation and purification of natural compounds, can solve the problems of affecting the yield of extracts and antioxidant effects, subsequent purification, complex refining steps, easy oxidative degradation of carnosic acid, etc. The effect of low, non-toxic odor and low production cost

Pending Publication Date: 2022-04-12
绿谷植源(清远)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of fat-soluble antioxidants by ethanol extraction usually requires the use of steam distillation to separate rosemary essential oil. During the production process, carnosic acid is easily oxidized and degraded, which affects the yield and antioxidant effect of the extract; supercritical CO2 extraction is used. Preparation of fat-soluble antioxidant, high production cost, single product and darker color, difficult to realize industrial production
Rosemary water-soluble antioxidants are usually by-products of rosemary essential oil and fat-soluble antioxidants. The content of rosmarinic acid in the extract is low, and the subsequent purification and refining steps are complicated and the production cost is high.

Method used

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  • Method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants
  • Method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants
  • Method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants

Examples

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preparation example Construction

[0032] In the preparation method for the present application, the dried rosemary raw material needs to be pulverized, passed through a 20-40 mesh sieve, and pulverized is conducive to increasing the contact surface area in the subsequent contact with the extraction solvent, so as to facilitate the target extraction However, the pulverized particles should not be too small, otherwise it is difficult for the extraction solvent to pass through the particle gap and sufficient elution cannot be achieved.

[0033] S02: using ethanol water as an extraction solvent to soak the raw material to form a solid-liquid mixture;

[0034] The water-soluble antioxidants in rosemary are mainly rosmarinic acid, which is an acid containing polyphenolic hydroxyl groups. It was first isolated and extracted from rosemary plants in 1958 and got its name. Rosmarinic acid is widely distributed, from lower mosses to higher dicotyledonous plants, mainly in Labiatae, Boraginaceae, Cucurbitaceae, Tiliaceae,...

Embodiment 1

[0052] A method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants, specifically comprising the following steps: (1) taking 10 g of dried rosemary stems and leaves, crushing and passing through a 20-mesh sieve; (2) using 70% (V / V) Soak the pulverized rosemary material in ethanol water, fully swell for 2 hours, and put it into a glass chromatography column, use 70% (V / V) ethanol aqueous solution as eluent, diameter-to-height ratio 1:5, elution flow rate 1BV / h for column chromatography extraction; (3) collect 10BV eluate and rotary evaporate to the water phase, add 10% (m / V) activated clay, decolorize at 40°C for 1h, concentrate and dry to obtain rosemary water-soluble antibiotic Oxidant; (4) Collect the precipitate in (3), add ethanol to dissolve it, add 10% (m / V) activated clay, decolorize at 40° C. for 1 hour, concentrate and dry to obtain rosemary fat-soluble antioxidant.

Embodiment 2

[0054] A method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants, specifically comprising the following steps: (1) taking 5 g of dried rosemary stems and leaves, crushing and passing through a 20-mesh sieve; (2) using 50% (V / V) Soak the pulverized rosemary material in ethanol water, fully swell for 2 hours, and put it into a glass chromatography column, use 50% (V / V) ethanol aqueous solution as eluent, diameter-to-height ratio 1:10, elution flow rate 2BV / h for column chromatography extraction; (3) collect 8BV eluate and rotary evaporate to the water phase, add 10% (m / V) activated carbon, decolorize at 40°C for 1h, concentrate and dry to obtain rosemary water-soluble antioxidant (4) Collect the precipitate in (3), add ethanol to dissolve, add 10% (m / V) activated carbon, decolorize at 40° C. for 1 hour, concentrate and dry to obtain rosemary fat-soluble antioxidant.

[0055] In summary, the present application simultaneously prepares rosemary wat...

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Abstract

The invention discloses a method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants. The method comprises the following steps: obtaining dry rosemary stems and leaves as raw materials; soaking the raw materials into a solid-liquid mixture by using ethanol water as an extraction solvent; eluting the solid-liquid mixture by adopting a chromatographic column to obtain a mixed extracting solution; separating water-soluble components and fat-soluble components in the mixed extracting solution by adopting a rotary evaporation method; the water-soluble component contains a rosemary water-soluble antioxidant; the fat-soluble component contains a rosemary fat-soluble antioxidant. The preparation method provided by the invention has the advantages of simple process, high efficiency, high extraction rate, large yield, easy realization of industrial and batch production, good product safety, recyclable solvent used in the preparation process, low production cost, less generation of hazardous wastes, and small environmental pollution.

Description

technical field [0001] The present application relates to the technical field of separation and purification of natural compounds, in particular to a method for simultaneously preparing rosemary water-soluble and fat-soluble antioxidants. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the use safety of artificial preservatives in food, cosmetics and daily necessities has attracted increasing attention. The development and application of pure natural antioxidants has become a research hotspot in the fields of food and chemical industry. Rosemary is a plant of the genus Rosmarinus in the family Lamiaceae. It is currently recognized as a safe and non-toxic plant with a high antioxidant effect. It is known as the third-generation green food antioxidant and has broad application prospects and huge market potential. . At present, commercially available rosemary antioxidants include fat-soluble antioxidants and water-so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C62/32C07C51/42C07C51/47C07C51/43C07C69/732C07C67/48C07C67/56C07C67/52A61P39/06
Inventor 阳成伟倪贺莫长佳
Owner 绿谷植源(清远)生物科技有限公司
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