Preparation method of deoxycholic acid
A technology of deoxycholic acid and cholic acid, which is applied in the fields of steroids, fermentation, organic chemistry, etc., can solve the problems of increasing the risk of high-temperature reactions, high requirements for the purity of cholic acid, and unsuitability for industrial production, so as to increase the total income efficiency and solvent utilization, good specificity and high specificity
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Embodiment 1
[0026] A preparation method of deoxycholic acid, specifically comprising the following steps:
[0027] S11. Dissolving 250 g of the cholic acid (40% content, containing 100.0 g of pure cholic acid, 244.75 mmol) with 200.0 g of 5% aqueous sodium hydroxide solution (250 mmol) to obtain a solution;
[0028] S12. Add dropwise the lysate to 2500ml of the 7-position oxidase culture solution (the oxidase concentration is 30U / ml) to carry out reaction conversion. During conversion, the temperature is controlled at 25°C, the pH is controlled at 8.3, and the solution is controlled at 8.3. The oxygen content is not less than 30%;
[0029] S13. Terminate the reaction after the reaction reaches a conversion rate of >99%, filter the converted mixture, raise the temperature of the filtrate to 40° C., then adjust the pH to 3 with 15% sulfuric acid at a temperature of 40° C., and stir and crystallize at the same time. Thereafter, the pH-adjusted mixture was stirred and grown at 50°C for 3 hou...
Embodiment 2
[0035] A preparation method of deoxycholic acid, specifically comprising the following steps:
[0036] S11. Dissolving 250 g of the cholic acid (40% content, containing 100.0 g of pure cholic acid, 244.75 mmol) with 200.0 g of 5% aqueous sodium hydroxide solution (250 mmol) to obtain a solution;
[0037] S12. Add the solution dropwise to 2500ml of 7-position oxidase culture solution to perform reaction conversion (the concentration of the oxidase is 40U / ml). During the conversion, the temperature is controlled at 27°C, the pH is controlled at 8.5, and the dissolved The oxygen content is not less than 30%;
[0038] S13. Terminate the reaction after the reaction reaches a conversion rate >99%, filter the converted mixture, raise the temperature of the filtrate to 45° C., then adjust the pH to 4 with 15% sulfuric acid at a temperature of 45° C., and stir and crystallize at the same time. Thereafter, the pH-adjusted mixture was stirred and grown at 57°C for 4 hours, and then filt...
Embodiment 3
[0044] A preparation method of deoxycholic acid, specifically comprising the following steps:
[0045] S11. Dissolving 250 g of the cholic acid (40% content, containing 100.0 g of pure cholic acid, 244.75 mmol) with 200.0 g of 5% aqueous sodium hydroxide solution (250 mmol) to obtain a solution;
[0046] S12. Add the solution dropwise to 2500ml of 7-position oxidase culture solution to perform reaction transformation (the concentration of the oxidase is 50U / ml). During the transformation, the temperature is controlled at 30°C, the pH is controlled at 8.6, and the solution The oxygen content is not less than 30%;
[0047] S13. Terminate the reaction after the reaction reaches a conversion rate of >99%, filter the converted mixture, raise the temperature of the filtrate to 50° C., then adjust the pH to 4 with 15% sulfuric acid at a temperature of 50° C., and stir and crystallize at the same time. Thereafter, the pH-adjusted mixture was stirred and grown at 55°C for 4 hours, the...
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