Ice-inhibiting foamed asphalt based on biological antifreeze protein and preparation method of ice-inhibiting foamed asphalt
A technology of antifreeze protein and foamed asphalt, which is applied to building structures, building components, building insulation materials, etc., can solve the problems of untimely deicing and snow removal, labor and material resources consumption, and insufficient durability. The production process is easy and the effect of environmental friendliness
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Embodiment 1
[0020] Weigh the following raw materials in parts by weight:
[0021] 90 parts of No. 70 base asphalt, 10 parts of water, 1 part of cetyltrimethylammonium bromide, 5 parts of Tenebrio antifreeze protein, 3 parts of mPEG-SG, 1 part of polysorbate-40, sodium phosphate buffer 1 part of solution (pH 6, concentration 0.9mol / L).
[0022] The preparation steps are as follows:
[0023] (1) Modify Tenebrio antifreeze protein. Put the weighed mPEG-SG and Tenebrio antifreeze protein into test tubes respectively, then pour an equal amount of sodium phosphate buffer solution into two centrifuge tubes, shake and mix well. The above two solutions were then poured into the same centrifuge tube and placed in a centrifuge, and reacted at 5° C. at a speed of 3000 r / min for 24 hours.
[0024] (2) Preparation of foamed asphalt. Heat No. 70 base asphalt to 150°C and place it in foaming equipment and keep it at 150°C. Add cetyltrimethylammonium bromide and water at 70°C in sequence at a stirring...
Embodiment 2
[0027] Weigh the following raw materials in parts by weight:
[0028] 95 parts of No. 90 base asphalt, 15 parts of water, 2 parts of diamine cationic asphalt emulsifier, 10 parts of antifreeze protein Tenebrio molitor, 6 parts of mPEG-NHS, 1.5 parts of polysorbate-60, sodium phosphate buffer 2 parts (pH 7, concentration 0.8mol / L).
[0029] The preparation steps are as follows:
[0030] (1) Modify Tenebrio antifreeze protein. Put the weighed mPEG-NHS and Tenebrio antifreeze protein into test tubes respectively, then pour an equal amount of sodium phosphate buffer solution into two centrifuge tubes, shake and mix well. The above two solutions were then poured into the same centrifuge tube and placed in a centrifuge, and reacted at 4° C. at a rotational speed of 2800 r / min for 24 hours.
[0031] (2) Preparation of foamed asphalt. Heat No. 90 base asphalt to 155°C and place it in foaming equipment and keep it at 155°C. At a stirring speed of 350r / min, add diamine-type cationic...
Embodiment 3
[0034] Weigh the following raw materials in parts by weight:
[0035] 90 parts of No. 90 base asphalt, 10 parts of water, 1.5 parts of cetyltrimethylammonium bromide, 8 parts of Tenebrio antifreeze protein, 4 parts of mPEG-SS, 1 part of polysorbate-60, potassium phosphate buffer 1.5 parts of solution (pH is 6, concentration is 1.2mol / L).
[0036] The preparation steps are as follows:
[0037] (1) Modify Tenebrio antifreeze protein. Put the weighed mPEG-SS and Tenebrio antifreeze protein into test tubes respectively, then pour an equal amount of potassium phosphate buffer solution into two centrifuge tubes, shake and mix evenly. The above two solutions were then poured into the same centrifuge tube and placed in a centrifuge, and reacted at 6° C. at a rotational speed of 2900 r / min for 23 hours.
[0038] (2) Preparation of foamed asphalt. Heat No. 90 base asphalt to 150°C and place it in foaming equipment and keep it at 150°C. Add cetyltrimethylammonium bromide and water at...
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