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Preparation method of culture medium taking agricultural wastes as raw materials, culture medium and application

A technology of agricultural waste and culture medium, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as difficult to deal with the environment, pollution, etc., achieve significant economic benefits, wide sources of raw materials, The effect of good strain culture effect

Pending Publication Date: 2022-04-19
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation method of the present invention not only solves the problem of environmental pollution caused by the difficulty in handling crop stalks and livestock and poultry excrement, realizes the recycling of resources, but also the prepared culture medium can provide microorganisms (especially fungi) with nutrients required for growth Substances and medium preparation methods are simple, providing a new path for the cultivation of medicinal and edible fungi

Method used

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  • Preparation method of culture medium taking agricultural wastes as raw materials, culture medium and application
  • Preparation method of culture medium taking agricultural wastes as raw materials, culture medium and application

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preparation example Construction

[0022] The invention provides a method for preparing a culture medium using agricultural waste as a raw material, comprising the following steps:

[0023] 1) Mix agricultural waste with water and liquefy under high temperature and high pressure to obtain a solid-liquid mixture;

[0024] 2) The solid-liquid mixture is subjected to solid-liquid separation, and the solid product, agar and water are mixed to obtain a culture medium;

[0025] The agricultural waste includes crop straw and / or excrement of livestock and poultry; the temperature of the high temperature and high pressure liquefaction is 180-250° C., the heating rate is 10° C. / min, and the pressure is 2-10 MPa.

[0026] In the invention, the agricultural waste is mixed with water and then liquefied under high temperature and high pressure to obtain a solid-liquid mixture. The agricultural waste includes crop stalks and / or livestock and poultry excrement. In the present invention, after mixing the solid product, agar a...

Embodiment 1

[0033]Add 200g of crop straw, 100g of livestock and poultry excrement, and 400g of water into the heating kettle, heat to 200°C, the heating rate is 10°C / min, the pressure is 2.7MPa, and the high temperature and high pressure liquefaction time is 24h;

[0034] After natural cooling, the material is discharged from the outlet of the heating kettle, and after being filtered by a vacuum pump, a high-temperature and high-pressure liquefied agricultural waste solid product is obtained;

[0035] Mix the obtained solid product with agar and water at a ratio of 1:6:400, 1:3:200 and 1:3:160 respectively, put it into a conical flask, sterilize it in a sterilizing pot, pour it into a culture In a dish, the culture medium was obtained after cooling.

Embodiment 2

[0037] A method for making a novel fungal culture medium, comprising the following steps:

[0038] Add 200g of crop straw and 400g of water into the heating kettle, heat to 200°C, the heating rate is 10°C / min, the pressure is 2.7MPa, and the high temperature and high pressure liquefaction time is 24h;

[0039] After natural cooling, the material is discharged from the outlet of the heating kettle, and after being filtered by a vacuum pump, a high-temperature and high-pressure liquefied agricultural waste solid product is obtained;

[0040] Mix the obtained solid product with agar and water at a ratio of 1:6:400, 1:3:200 and 1:3:160 respectively, put it into a conical flask, sterilize it in a sterilizing pot, pour it into a culture In the dish, after cooling, spray 1g / ml starch aqueous solution to obtain culture medium.

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Abstract

The invention relates to a preparation method of a culture medium taking agricultural wastes as raw materials, the culture medium and application, and belongs to the technical field of microbial culture. The agricultural waste and water are mixed and then subjected to high-temperature and high-pressure liquefaction, and a solid-liquid mixture is obtained; and carrying out solid-liquid separation on the solid-liquid mixture, and mixing a solid product, agar and water to obtain the culture medium. The preparation method provided by the invention not only solves the problem of environmental pollution caused by difficult treatment of crop straws and livestock and poultry excrements and realizes resource recycling, but also the prepared culture medium can provide nutrient substances required by growth of microorganisms (especially fungi), and the culture medium is simple in preparation method and low in cost. A new path is provided for the culture of medicinal and edible fungi.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a preparation method, culture medium and application of a culture medium using agricultural waste as a raw material. Background technique [0002] As a developing agricultural country, my country is the country with the largest grain output in the world. While the grain harvest is bumper, the disposal of straw has become an imminent problem. At present, restricted by various factors such as technology, capital, and concepts, the main treatment method for straw is still incineration. However, crop straw contains nitrogen, phosphorus, potassium, carbon and hydrogen elements and organic sulfur. In particular, the freshly harvested straw is not yet dry, and incomplete combustion will produce a large amount of nitrogen oxides, sulfur dioxide, hydrocarbons and smoke, and may also produce secondary pollutants such as ozone under the action of sunlight. These substanc...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/22C12R1/645
CPCC12N1/14C12N1/22
Inventor 高岩赵远崔学军施维陆海彦
Owner JILIN UNIV